Leica TCS SP5 X, Руководство пользователя

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10.2.5 The Pinhole and Its Effects............................................................................. 93
10.2.6 Image Detail and Raster Settings................................................................... 95
10.2.7 Signal and Noise ............................................................................................ 99
10.2.8 Profile Cuts................................................................................................... 101
10.3 Multiparameter Fluorescence......................................................................... 102
10.3.1 Illumination ................................................................................................... 102
10.3.2 Beam Splitting .............................................................................................. 104
10.3.3 Emission Bands............................................................................................ 104
10.3.4 Crosstalk ...................................................................................................... 104
10.3.5 Sequential Scanning .................................................................................... 105
10.3.6 Unmixing ...................................................................................................... 105
10.4 3D Series........................................................................................................ 106
10.4.1 Z-stack.......................................................................................................... 106
10.4.2 Section Thicknesses .................................................................................... 106
10.4.3 Distances...................................................................................................... 107
10.4.4 Data Volumes............................................................................................... 107
10.4.5 Depictions..................................................................................................... 108
10.4.5.1 Gallery ..................................................................................................... 108
10.4.5.2 Movie ....................................................................................................... 108
10.4.5.3 Orthogonal Projections ............................................................................ 109
10.4.5.4 Rotated Projections ................................................................................. 110
10.5 Time Series .................................................................................................... 110
10.5.1 Scan Speed.................................................................................................. 110
10.5.2 Points ........................................................................................................... 111
10.5.3 Lines............................................................................................................. 111
10.5.4 Planes .......................................................................................................... 111
10.5.5 Spaces (Time-Space)................................................................................... 111
10.5.6 FRAP Measurements ................................................................................... 112
10.6 Spectral Series ............................................................................................... 112
10.6.1 Data Acquisition and Utilization.................................................................... 112
10.6.2 About Spectral Resolution............................................................................ 112
10.7 Combinatorial Analysis................................................................................... 112
11. Care and Maintenance .................................................................................... 115
11.1 General........................................................................................................... 115
11.2 Cleaning the Optical System .......................................................................... 115