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General information 

The temp files during scanning are stored in C:\Temp\ which is generally fastest 
on the C: drive.  If the system crashes you may be able to recover images from 
there. 

On the Belkin USB monitor selection box under the monitors, the LED shows 
yellow when the Leica SP8 Master PC monitor is active and green when the 
PicaQuant Slave PC monitor is active. There’s a small button on the front left of 
the USB box to switch between PC monitors, but this shouldn’t be necessary as 
the mouse curser can move across both PC desktops to select that PC for 
keyboard input and mouse control

 

Shut down the PicaTech Slave PC last first and switch on the Slave PC last (the 
Leica SP8 Master PC goes on first). Log in with the same WTCHG account on both 
PCs. 

In the Leica LSM software, if you set the WWL to Constant Percent, at say 10% 
the actual laser power will vary depending on the laser-line selected. If you set 
the WWL to Constant Power, the power output of the WWL will be adjusted 
automatically to give the same power level at every wavelength, so say 10% will 
be approximately the same laser power at every laser line. 

Pulse Picker gives the best image quality at the highest repetition rate. 

Make sure the XC-Port is in ‘mirror’ position to block off any of the SMD 
detectors. 

When setting up a Tile Scan with the motorised stage, be careful not to accidently 
click on the XY stage grid pad as the stage will move rapidly to that XY position. 

There are hardbound manuals for the DMI6000 microscope and the Leica SP8 
software/hardware. These are also available as pdf files in the both Master and 
Slave PC hard drives.  

 

 

Содержание SP8-X

Страница 1: ...Page 1 Wellcome Trust Centre for Human Genetics Cellular Imaging Microscopy Core The Leica SP8 X SMD Confocal Abridged INSTRUCTIONS...

Страница 2: ...ed scanning XY Stage including Mark Find and Autostitching Mosaic Modules Passive anti vibration microscope air table AOBS filter free scanhead WLL2 tuneable pulsed single diode laser with 200 lines f...

Страница 3: ...rst Log in with the same WTCHG account on both PCs In the Leica LSM software if you set the WWL to Constant Percent at say 10 the actual laser power will vary depending on the laser line selected If y...

Страница 4: ...ectives never change from an oil to an air objective when focussed on the specimen under oil you can start with the low power air objective and then move up to the hi power oil objective The large HC...

Страница 5: ...Page 5...

Страница 6: ...when looking down the microscope press the diochroic filter selection button e g DAPI and then shutter Also use the fluorescence transmitted light toggle button on the bottom left side of the microsc...

Страница 7: ...Page 7...

Страница 8: ...Page 8 SmartMove XYZ Stage Controller...

Страница 9: ...Page 9...

Страница 10: ...ight Red LED rocker switch Note this controls power to the PicaQuant PC and both PC monitors 8 The Leica SP8 Master PC monitor will switch on and this will start up the PicaQuant Slave PC automaticall...

Страница 11: ...the SP8 Master PC first 6 Shut Down the SP8 Master PC Transfer any data files to the network drives first 7 Turn the Laser Emission key to off There s no argon laser to cool down and the White Light L...

Страница 12: ...e any microscope imaging problems or queries just ask a member of the Microscopy Core staff for help There is additional help and advice on our Core web pages http www well ox ac uk microscopy WTCHG L...

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