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General information
The temp files during scanning are stored in C:\Temp\ which is generally fastest
on the C: drive. If the system crashes you may be able to recover images from
there.
On the Belkin USB monitor selection box under the monitors, the LED shows
yellow when the Leica SP8 Master PC monitor is active and green when the
PicaQuant Slave PC monitor is active. There’s a small button on the front left of
the USB box to switch between PC monitors, but this shouldn’t be necessary as
the mouse curser can move across both PC desktops to select that PC for
keyboard input and mouse control
Shut down the PicaTech Slave PC last first and switch on the Slave PC last (the
Leica SP8 Master PC goes on first). Log in with the same WTCHG account on both
PCs.
In the Leica LSM software, if you set the WWL to Constant Percent, at say 10%
the actual laser power will vary depending on the laser-line selected. If you set
the WWL to Constant Power, the power output of the WWL will be adjusted
automatically to give the same power level at every wavelength, so say 10% will
be approximately the same laser power at every laser line.
Pulse Picker gives the best image quality at the highest repetition rate.
Make sure the XC-Port is in ‘mirror’ position to block off any of the SMD
detectors.
When setting up a Tile Scan with the motorised stage, be careful not to accidently
click on the XY stage grid pad as the stage will move rapidly to that XY position.
There are hardbound manuals for the DMI6000 microscope and the Leica SP8
software/hardware. These are also available as pdf files in the both Master and
Slave PC hard drives.
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