CIAN Leica SP8 – short instructions, version 3.1, September 2015
page 10 of 10
Appendix: Technical data for Leica SP8
Microscope:
Leica SP8 point-scanning confocal system, on a Leica DMI6000B inverted microscope, fully
motorized, with Leica “SuperZ” Galvo stage, four spectral fluorescent light detectors (three PMT,
one HyD high sensitivity detector), one transmitted light detector (PMT)
Objectives:
Position
Objective
DIC
1
10x
/0.4
dry
HC PL APO 1.x/0.40 CS
(prism not
installed)
2
20x
/0.7
dry
HC PL APO 20x/0.70 CS
DIC
3
40x
/0.85
dry
HCX PL APO 40X/0.85 CORR CS,0.11-
DIC
4
40x
/1.1
water
HC PL APO 40x/1.10 W CORR CS2
DIC
5
40x
/1.3
oil
HC PL APO 40x/1.30 Oil CS2
DIC
6
63x
/1.4
oil
HC PL APO 63x/1.40 OIL CS2
DIC
Confocal mode: Lasers, no fluorescence emission filters (all spectral detectors):
Lasers (all diode)
Typical fluorophore
405nm, 50mW, DMOD
DAPI
448nm, 40mW, AOTF
CFP
488nm, 20mW, AOTF
AF488, GFP, YFP
552nm, 20mW, AOTF
TRITC, Cy3 Texas Red, RFP
638nm, 30mW, AOTF
AF633, Cy5, FarRedFP
Laser modulation by AOTF (acusto-optic tunable filters), or DMDO (direct modulation of laser
intensity)
Wide-field fluorescence:
Light source: Leica EL6000 metal-halide lamp
Fluorescence filter cubes, Leica:
Filter set
Excitation filter
Dichroic
Emission filter
Typical
fluorophore
A
BP340-380
400
LP425
DAPI
I3
BP450-490
510
LP515
AF488, GFP
N2.1
BP515-560
580
LP590
TRITC, RFP
