65
Leica RM2245
7.
Troubleshooting
1. Thick/thin sections
The sections alternate between
being thick and thin. In extreme
cases, there are no sections
whatsoever.
• Check the clamping positions
and repeat the clamping proc-
ess
• Laterally displace the knife hold-
er or insert a new blade.
• Insert a new pressure plate or
use a new knife holder.
• Methodically experiment with
larger clearance angle settings
until you have found the opti-
mum angle.
Problem
Corrective action
Possible cause
• Insufficient clamping of blade,
knife holder, specimen or orien-
tation.
• The blade is dull.
• The pressure plate is damaged
or incorrectly adjusted.
• Clearance angle of the knife/
blade too small.
• The blade is dull.
• The specimen is too warm.
• The sectioning speed is too fast.
• There is an accumulation of
paraffin on the rear pressure
plate of the knife holder.
3. “Stripes” in sections
For knife holder E
2. Section compression
The sections are very com-
pressed, show folds or are
squeezed together.
• Adjust the sectioning speed
and/or section thickness when
sectioning. Select a thinner
section.
• Remove paraffin from this area
on a regular basis.
• Use another area of the blade or
a new blade.
• Cool the specimen before
sectioning.
• Reduce the sectioning speed.
• Too great of a sectioning force
was applied.
4. Noises during sectioning
The knife “sings” when section-
ing hard specimens. The sec-
tions show scratches or chatter
marks.
• The sectioning speed is too fast.
• The clearance angle is too wide.
• Insufficient clamping of speci-
men clamp and/or knife holder.
• Select a slower sectioning
speed.
• Methodically reduce the clear-
ance angle setting until you
have found the optimum angle.
• Check all screw and clamp
connections on the specimen
holder system and the knife
holder. If necessary, tighten the
levers and screws.
7.2 Possible faults
5. High blade consumption
Содержание RM2245
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