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Instruction Manual

Leica RM 2155 V1.1 English – 11/2000
Always keep this manual near the instrument!

Read carefully prior to operating the instrument!

Leica RM 2155

Rotary  Microtome

Содержание RM2155

Страница 1: ...Instruction Manual Leica RM 2155 V1 1 English 11 2000 Always keep this manual near the instrument Read carefully prior to operating the instrument Leica RM 2155 Rotary Microtome...

Страница 2: ......

Страница 3: ...r ticular no liability whatsoever is accepted for any fi nancial loss or consequential damage caused by or related to compliance with statements or other infor mation in this manual Statements drawing...

Страница 4: ...imen 26 10 Universal knife holder base 27 10 1 Repositioning the knife holder base 27 10 2 Mounting the knife holder 28 10 3 Adjusting the clearance angle 28 11 Daily operation 29 12 Trouble shooting...

Страница 5: ...check Push the emergency stop switch The sectioning motor will stop immediately E STOP red on the control panel illuminates indicat ing that the emergency stop function is activated To deactivate turn...

Страница 6: ...ally blocked LOCK yellow on the control panel Fig 19 illuminates To release the brake push the locking lever 1 to the rear LOCK extinguishes The sectioning motor can then be activated again Centering...

Страница 7: ...ot be removed or modified Operating the instrument Take care when handling microtome knives and disposable blades The cutting edge is extremely sharp and can cause severe injury Never leave knives and...

Страница 8: ...turn the instrument on before it is completely dry Maintenance Only authorized and qualified service per sonnel may access the internal compo nents of the instrument for service and re pair Turn the i...

Страница 9: ...rotome Section thickness setting 0 5 60 m from 0 5 to 1 m in 0 5 m increment from 1 to 20 m in 1 m increments from 20 to 60 m in 5 m increments Horizontal specimen feed 27 mm via step motor Vertical s...

Страница 10: ...ht Basic instrument Width including handwheel 400 mm Width excluding handwheel 300 mm Depth 550 mm Overall height 290 mm Working height knife edge 100 mm Net weight 44 2 kg External control panel opti...

Страница 11: ...or section thickness setting Handwheel with centering handle Orienting specimen clamping system Universal cassette clamp UCC Control panel Clamping lever for disposable blade TRIM STEP button Clamping...

Страница 12: ...a motorized rotary microtome The specimen feed system with zero backlash and maintenance free cross roller guides and the step motor operated coarse feed system are located in a dustproof plastic hous...

Страница 13: ...able Lightly lift the transport platform at the front Loosen the screw 6 on the underside of the trans port platform with the Allen key size 8 7 provided and remove the washer Cut off the plastic cove...

Страница 14: ...ooth operation the instrument must be set up with at least 10 cm distance from walls and furniture The installation site must meet the following require ments stable vibration free laboratory table vi...

Страница 15: ...hen lock automatically when released 7 1 2 Mounting the knife holder base To release turn the lever 1 counterclockwise Place the knife holder base 2 with the two clamp ing levers 3 and 4 mounted in pl...

Страница 16: ...ch nor the external con trol panel is connected the instrument will be inoperational Connecting the mains cable Before connecting the mains cable check if the mains switch 3 at the rear of the instrum...

Страница 17: ...elector is set to the voltage by the factory as indicated on the order form The name plate reads the power rating of the volt age selector setting A change in the setting is generally not re quired Sh...

Страница 18: ...switch at the rear This is followed by a beep The instrument initializes The software version is displayed for approx two sec onds The display reads the section thickness previously se lected e g 0 5...

Страница 19: ...ton is held down to move the specimen away from the knife HOME illuminates on reaching the rear limit HOME position STOP green flashes while the coarse feed button is held down to move the specimen to...

Страница 20: ...y activation of CLEAR even if the display indicates the section or trimming thickness Selecting the sectioning mode Two motorized sectioning modes are available single stroke and continuous stroke Onc...

Страница 21: ...left side of the microtome The control knob has a graduated collar Speed range 0 0 5 to 420 mm s Scale range 0 10 8 2 3 Emergency stop switch The red emergency stop switch is located on the front Push...

Страница 22: ...ed section thickness Selecting the TRIM STEP To select the next TRIM STEP increment turn the knob clockwise to the next lock in position The selected value is indicated in the display Activating the T...

Страница 23: ...6 Indication of remaining horizontal feed STOP green illuminates approx 1 mm before the specimen reaches the front limit A beep sounds for approx 2 seconds Sectioning is interrupted and the specimen...

Страница 24: ...T yellow no longer illuminates When retraction is activated the display reads on To deactivate push the coarse feed button at the rear Toactivate pushthecoarsefeedbuttonatthefront Push the CLEAR butto...

Страница 25: ...l stop automatically after each section in the upper limit Sectioning mode 2 Continuous stroke Press the foot switch once to start sectioning If the foot switch is held down for more than half a secon...

Страница 26: ...guide 2 of the specimen clamp into the mount 3 from the left To clamp turn the clamping lever 1 clockwise to the limit stop 9 2 Orienting the specimen Rotate the handwheel to position the specimen cla...

Страница 27: ...r base North South North Southdisplacementenablestheapproachofthe knife holder to the sample as close as possible To release turn the clamping lever 1 on the left sideofthemicrotomebaseplatecounterclo...

Страница 28: ...ife holder A reference point is provided on the hand rest Moreover a reference line is provided on the right side of the knife holder base to adjust the angle when the hand rest is removed Torelease t...

Страница 29: ...all section waste to the section waste tray Empty the section waste tray Clean the instrument see chapter 13 Cleaning Turn the instrument on with the mains switch Always lock the handwheel prior to m...

Страница 30: ...l the other section thickness settings except the 0 5 m setting This is caused by an operating er ror All functions of the instrument are inoperational Do not press any button Turn the instrument off...

Страница 31: ...by the technical service 12 1 5 Sectioning motor cannot be restarted The sectioning motor is blocked and cannot be re started The sectioning motor was subject to a short term over load and was cut of...

Страница 32: ...ower sectioning speed Select lower sectioning speed Clearance angle adjustment systematically decrease the clearance angle until the optimum adjustment is ob tained Check if all levers are locked and...

Страница 33: ...the specimen towards the rear limit 2 Push the coarse feed button at the rear to move the specimen towards the rear limit 1 Mains cable not properly connected 2 Mains fuses defective 1 Loss of the ret...

Страница 34: ...panel 1 Press the foot switch shortly 2 Remove foot switch Connect the foot switch adapter Recon nect the foot switch 3 Release both buttons simulta neously if possible 1 Please call the technical se...

Страница 35: ...Remove the hand rest for cleaning Remove the knife holder and knife holder base for cleaning Only use mild commercial detergents or soap solution for cleaning Do not use solvents that contain acetone...

Страница 36: ...act your local Leica service center Clean the instrument every day Lubricate the following parts with the oil No 405 once a month 1 2 drops will be sufficient T piece 1 on the microtome base plate Gui...

Страница 37: ...ent off with the mains switch and pull the mains plug before changing the voltage setting Turn the instrument off with the mains switch Disconnect the mains plug from the mains power outlet Place the...

Страница 38: ...mains fuses are located in the voltage selector housing 3 above the mains switch at the rear of the instrument Remove the voltage selector housing 3 as de scribed under 14 2 Remove the fuses 4 Mount...

Страница 39: ...and pull it out together with the spring 5a and the pin 5b To remove turn the eccentric bolt 6 counterclock wise Loosen the 2 screws 7 and 2 screws 8 which are accessible through the holes 9 and remov...

Страница 40: ...blocks In addition it accommodates the foil clamps Turntheknurledknob 1 counterclockwisetomove the movable jaw 2 downward Mount the sample as required Turn the knurled knob 1 clockwise to move the mov...

Страница 41: ...clockwise To orient the sample introduce the pin 9 in one of the holes 10 and turn counterclockwise To fix the orientation introduce the pin 9 again in one of the holes 10 and turn clockwise 15 2 4 Ve...

Страница 42: ...hefoilclampinthestandardspecimenclamp as shown Turn the knurled knob 1 clockwise to securely clamp the foil clamp in the standard specimen clamp Clamping of flat samples To clamp flat samples replace...

Страница 43: ...nthestandardspecimenclamp so that the bevelled surface 7 on the back of the foil clamp points to the right or left Turn the knurled knob 1 clockwise to securely clamp the foil clamp in the standard sp...

Страница 44: ...tlateraldisplacementfunc tion described above Loosen the 2 screws 1 with the provided screw driver and remove the cover plate 2 Insert the backlighting 3 in the recess at the rear of the knife holder...

Страница 45: ...knife and dur ing breaks always lock the handwheel and cover the cutting edge with the knife guard Microtome knives and disposable blades Take care when handling microtome knives and disposable blades...

Страница 46: ...knife support bar via the height adjust ment screws to the lowest position to prevent dam age to the cutting edge while inserting the knife Unscrew the knife clamping screws 2 counter clockwise as far...

Страница 47: ...t bar Inserting the knife Turn the knurled wheels 6 on the right and left of the knife holder in opposite directions to the front to move the knife support bar via the height adjust ment screws to the...

Страница 48: ...of the knife holder by using an Allen key size 4 Remove the pressure plate 5 Attach the new pressure plate by tighten the 4 screws 4 Cleaning The clamping plate 7 can be removed for cleaning To remove...

Страница 49: ...g lever 2 up Cleaning The clamping plate 7 can be removed for cleaning To remove the blade relocate the clamping lever 2 down Carefully remove the blade Pull out the clamping lever 2 Remove the clampi...

Страница 50: ...f the control panel The control panel enables to select the two sectioning modes single continuous stroke and to start and stop motorized sectioning Start motorized sectioning After selecting one of t...

Страница 51: ...0402 08320 Holder for EM specimen holder 0502 29968 Segment arc with adapter for EM specimen holder 0502 29969 Specimen clamping system non orienting 0502 29963 Specimen clamping system orienting 0502...

Страница 52: ...t and or faulty operation invalidate the warranty and any claims based thereon and likewise Leica will not assume liability for any con sequential damage Disposal The instrument or parts of the instru...

Страница 53: ...53 Leica RM 2155 Rotary Microtome Notes...

Страница 54: ...54 Instruction Manual V1 1 01 2001 Notes...

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