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8. Imaging Procedure for Leica DM4000 B/DM5000 B
Notes:
• The microscope automatically selects the
correct light ring in the condenser.
• When selecting the phase contrast method,
the aperture diaphragm is opened completely
and may not be adjusted. To avoid errors in
operation, the function keys for setting the ap-
erture diaphragm (AP) are locked.
8.1.3 Dark Field (TL)
• Switch to the transmitted light axis (TL) by
pushing the
TL/IL
button.
• Select the
DF
(dark field) contrast method.
Do so by pressing the
DF
variable key.
Alternatively: Press the
CHANGE TL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display indicates
DF
.
The dark field ring (dark field stop) is set au-
tomatically.
• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
• Bring the image into focus using the focus dial
and set the brightness using the
INT
function
key.
Notes:
• The maximum objective aperture which may
be used for dark field is
0.75
. All objectives
with greater aperture are automatically
blocked for this procedure ("DF" flashes in the
display).
• The microscope automatically selects the
correct light ring in the condenser.
• When selecting the dark field method, the
aperture diaphragm is opened completely and
may not be adjusted. To avoid errors in opera-
tion, the function keys for setting the aperture
diaphragm (AP) are locked.
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