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Leica DM IRM, Инструкция по эксплуатации

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Issued in 1998 by:

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents:

MQM Marketing, Product Management

Tel.

+49 (0) 64 41-29 22 39

Fax

+49 (0) 64 41-29 24 64 or 22 55

e-mail [email protected]

Содержание DM IRM

Страница 1: ...Leica DM IRM Inverted Research Microscope for Material Testing Instructions...

Страница 2: ...ca Microsystems Wetzlar GmbH Ernst Leitz Stra e D 35578 Wetzlar Germany Responsible for contents MQM Marketing Product Management Tel 49 0 64 41 29 2239 Fax 49 0 64 41 29 24 64 or 22 55 e mail Klaus L...

Страница 3: ...ent of Leica Microsystems Wetzlar GmbH The information contained in the following docu mentation represents the latest stage of techno logy and knowledge We have composed the texts and illustrations w...

Страница 4: ...t up and operation 4 1 Coaxial coarse and fine focusing 36 37 4 2 Observation tubes 38 39 4 3 Tube module 1x magnification changer 1x 1 5x B 40 4 4 Lateral photo TV exit 41 4 5 Optical outfits 42 46 4...

Страница 5: ...separate manuals This manual is an integral part of the product and must be read carefully before you start using the microscope Text symbols and their meaning Special safety information is indi cate...

Страница 6: ...on short description and technical data of the microscope 7 Fig 2 Leica DM IRM inverted microscope 1 Lamphousing 107 for left handed operation 2 Collector adjustment for lamp centration 3 Lamp centrat...

Страница 7: ...xit 100 vis 20 vis 80 TV Order no 571006 Leica DMIRM with tube optics oo 1x 1 5x HC magnification changer with lateral photo TV exit 100 vis 100 TV Order no 571007 Leica DMIRM with tube optics oo 1x 1...

Страница 8: ...ake sure not to damage the sensitive damping feet on the underneath of the microscope n b Do not connect microscope and peripherals to mains yet see section 3 2 Assembly Installation site Make sure th...

Страница 9: ...on any accesso ries connected to the microscope which have their own and or a different power supply can be given the same ground conductor poten tial Please consult our servicing personnel if you int...

Страница 10: ...anywhere else where water may get inside them n b Before changing fuses or lamps always turn the mains switch off and disconnect the mains cable n b Protect the microscope from major tempera ture flu...

Страница 11: ...06Z and magnifies the image of the focal point of the lamp by the fac tor 2x in the entrance pupil of the objective This results in substantially higher illumination intensity for fluorescence 3 Plug...

Страница 12: ...adjustment 4 Dovetail ring mount 5 Reflector adjust ment not visible Fig 8 Mirror housing and illumination telescope 1 Mirror switching lever 2 Lateral lamphousing mount with fixing screw 3 Back lamph...

Страница 13: ...the base towards the front 10b 1 Caution hot surface Without removing its protective cover put a new lamp into the base without tilting as far as it will go n b Leave the protective cover on the lamp...

Страница 14: ...plug slightly out of the socket 11 11 and flip up lid 11 1 Move the collector to the front and lift the defect lamp out of the base 11 2 11 3 14 1 For convenience the lamp holder can be re moved from...

Страница 15: ...mask when assembling Xe burners risk of explosion Type Average life span Hg ultra high pressure lamp 50W A C 100 h Xe high pressure lamp 75W D C 400 h Hg ultra high pressure lamp 100W D C 200 h Hg ul...

Страница 16: ...on the lower lamp base is visible It must be readable i e not upside down 2 the upper base is labelled with the letters UP 17 Fig 13 Lamp holders for gas discharge lamps 1 Upper clamp 2 Seal point of...

Страница 17: ...o the front position with the focusing knob 14 1 n b Remove the protective covering from the burner 13 7 Put the lamp holder with burner inserted into the lamphousing and secure with the screws 11 10...

Страница 18: ...down the stage with Allen screws To assemble the square insert plate 16 1 1 Insert the corner of the insert plate that is marked red 16 2 at an angle from above into the corner of the stage that is a...

Страница 19: ...mple Please handle the round stage inserts 18 with care The thin inner steel rings 18 1 should not be subjected to any pressure or dropped Rough treatment can cause deformation Simple stage plate The...

Страница 20: ...e setting 55 75 mm Using a hexagonal screwdriver slacken the clamp screw 20 1 21 1 22 1 on the side of the tube change mount on the stand and remove the black cap Mount the tube so that the guide pin...

Страница 21: ...the DM IRM The tube adapter IR HC is mounted on the tube change mount of the stand and stabilised by tightening the clamp screw 23 4 n b Hold on to the tube adapter until the clamp screw is tightened...

Страница 22: ...hoto TV port Fig 24 Leica DMR HC tubes 1 HC BSA 25 2 HC FSA 25 P PR 3 Beamsplitter switch rod 4 Mount for photo adapter tube 5 Clamp for photo adapter tube 6 Clickstop position for Pol eyepieces 7 Soc...

Страница 23: ...rresponding standard magni fication engraved in the front of the nosepiece e g objective 5x with standard magnification 50 26 2 Unoccupied nosepiece positions must be closed with the supplied screw ca...

Страница 24: ...light intensity when changing between bright and darkfield It is not to be recommended for observing dark objects or for polarisation or interference con trast Nor is it necessary for purely brightfie...

Страница 25: ...26 Fig 29 BF reflector 1 Neutral plane glass beamsplitter 2 N16 neutral density filter Fig 30 DF reflector Fig 31 Smith reflector Fig 32 ICR reflector 1...

Страница 26: ...ism disc with label plates 34 5 Prism A for objectives N PLAN 5x 10x Prisms D and D1 both for objectives N PLAN 20x 50x 100x and HC PL FLUOTAR 5x 100x Differences between prism D and D1 Prism D is the...

Страница 27: ...ation direction is intended for use on the DM IRM Preselect the vibration direction 0 east west on the dial 37 2 Together with analyser IRM ICR P 90 north south the polariser and analyser are in the c...

Страница 28: ...hich ensure the cor rect position in the light path in or out n b If using high intensity gas discharge lamps e g Xe 75W always insert a Pol protection filter 504079 in the lamphousing 39 1 to prevent...

Страница 29: ...fix in position with the 4 screws 43 1 Do not hold on to the TL illumination column when screwing it down so that optimal alignment to the optical axis is guaranteed Fig 41 Back view of microscope 1...

Страница 30: ...g 44 1 Carefully remove the defect lamp from the pin base Insert a new 12V 100W lamp still in its protective cover into the pin base 45 1 as far as the stop n b Leave the lamp s protective cover on un...

Страница 31: ...49 5 and Condenser range See Leica DMIRB manual Assembling the 0 30 S70 condenser Tilt the TL illumination column to the back 46 1 Insert the 0 30 S70 condenser 46 4 with the top pointing towards the...

Страница 32: ...cides with condenser height marking S23 2 Dovetail guide 3 Condenser height markings S1 S23 and S70 4 Condenser holder 5 Clamp screw for securing the condenser holder 6 Clamp screw for field diaphragm...

Страница 33: ...ted into the holder at the factory If you are retrofitting filters yourself assemble as follows Slacken the clamp screws Fig 51 1 and re move the filter holder Put the filters into the holder Fig 52 M...

Страница 34: ...Field diaphragm module 2 Field diaphragm adjustment 3 Clamp screw for securing the field diaphragm module Fig 51 Assembly of filters 1 Clamp screws for securing the filter holder Fig 52 Assembly of t...

Страница 35: ...gh magnifications and short working distances from 50x upwards are used the specimen and the stage insert may easily be raised and tilted When the specimen is scanned the front lens of the objective m...

Страница 36: ...he microscope 1 Coarse drive 2 Fine drive with scale division 3 Objective nosepiece with nosepiece focusing 4 Specimen stages with 3 point support Fig 54 Specimen stage with inserts 1 Inner hole of th...

Страница 37: ...croscope superim pose Only one single circular clear image is seen Compensation of defective eyesight For viewers with normal eyesight and those wear ing eyeglasses the eyelenses of the eyepieces are...

Страница 38: ...39 Fig 55 Binocular tube HCI B 22 1 Eyepiece tubes 2 Eyepieces 3 Anti glare protection push back eyecups Fig 56 Trinocular tube HCI 3T 22 1 Photo TV exit 2 Switch rod for light path 3 2 1 1 2...

Страница 39: ...module oo 1x 1 5x Bertrand lens By turning the wheel 58 1 it is either possible to engage the Bertrand lens or to focus with the focusing lever 58 2 With the Bertrand lens the back focal plane of the...

Страница 40: ...th light path 100 visual or 20 visual 80 side exit or configuration with light path 100 visual or 100 side exit Position of switching rod 59 1 pulled out side exit switched on 80 or 100 light Position...

Страница 41: ...0 D Correction Reproduction Numerical Bright and dark Infinity Coverglass ICR class ratio aperture field objective corrected corrected prism type Eyepiece lettering 10x magnification 20 field of view...

Страница 42: ...if they are brightfield objectives and com bined with the spacer ring 32 RMS Depending on the year the objective was made the engraved magnifications may deviate by the factor 0 8x as the new DMIRM i...

Страница 43: ...x 0 15 D FWD 12 00 mm Order no 506504 HC PL FLUOTAR 10x 0 30 D FWD 11 00 mm Order no 506505 HC PL FLUOTAR 20x 0 50 0 D FWD 1 27 mm Order no 566500 HC PL FLUOTAR 50x 0 80 0 D FWD 0 50 mm Order no 56650...

Страница 44: ...D FWD 0 30 mm Order no 566031 PL FLUOTAR objectives HC PL FLUOTAR 5x 0 15 BD D FWD 12 20 mm Order no 566506 HC PL FLUOTAR 10x 0 30 BD D FWD 11 00 mm Order no 506503 HC PL FLUOTAR 20x 0 50 BD 0 D FWD 1...

Страница 45: ...er no 506 808 62 2 n b When wearing eyeglasses remove the anti glare protection of the eyepieces or push back the eyecups or you will not be able to see the whole field of view The eyepiece field of v...

Страница 46: ...51 Graticule with 10x10x 0 1mm grid divisions Order no 506954 Graticule with 10x10x1mm grid divisions Order no 506955 Graticule with crosshair Order no 506953 Graticule with 10mm 200 divisions Order n...

Страница 47: ...ables exact comparison of grain sizes The centre circle can also be used for grain size determinations with the circle segment or area count methods The graticule also has a reference length for the S...

Страница 48: ...with a blunt blade Insert the graticule with the coated side pointing downwards towards the objective Lettering must be shown the right way round Screw the retainer ring and the lower part of the eyep...

Страница 49: ...a life expectancy of approx 50 hours when the lamp current is turned on full We therefore recommend reducing the load cur rent especially when not actually using the microscope This considerably incr...

Страница 50: ...ce of the objective can be clearly seen 4 Adjust the lamp collector 68 4 until you see the structure of the lamp filament The fila ment image is divided into two with a pale stripe in the middle 69 Pl...

Страница 51: ...e and the reflection of the reflector are focused sepa rately and aligned to each other Either of the above methods can be used for im aging the lamp filament or arc Centration of 12V 100W halogen lam...

Страница 52: ...70 1 5 move the direct image of the arc to the middle of the cen tration area with the bright tip of the arc the focal spot of the cathode just off centre Then focus the reflection 70 3 and using the...

Страница 53: ...ing the image intensity Centring the field diaphragm Turn a low to medium objective magnification 10x 20x into the light path and focus a strongly reflecting specimen e g surface mirror via the BF ref...

Страница 54: ...in the mirror housing 8 2 other positions for filters of 50mm diameter in holder in a special intermediate piece 75 2 which can be installed between the lamphousing and the lamp mount or mirror housi...

Страница 55: ...56 Fig 75 Intermediate filter holder 1 Light filters 50 mm diameter 2 Intermediate piece with filter spaces Fig 74 Side view of microscope 1 3 levers for operating filters 1 2 1...

Страница 56: ...ing of the field diaphragm remains the same for all objective magnifications The aperture diaphragm influences the resolution contrast and field depth of the microscope image It should be opened just...

Страница 57: ...58 Fig 77 Setting the aperture diaphragm entrance pupil of the objective 1 Aperture diaphragm open 2 Aperture diaphragm closed by 1 3 3 Aperture diaphragm closed 1 2 3...

Страница 58: ...ight path and adjust the illumination to a medium intensity set ting n b Make sure the front lens of the objective is clean as impurities on the front lens greatly impair the quality of the darkfield...

Страница 59: ...the compensator is about 14 This polariser is particularly useful for fine variati ons of the pol optic colour contrast of anisotropic metal surfaces e g aluminium zircon titanium Incident light polar...

Страница 60: ...y possible by considerably altering the position of the ICR prism turret 76 2 Swing in an ICR prism by rotating the prism turret 76 2 and set ICR contrast on the prism turret with the knurled knob 34...

Страница 61: ...e diaphragm 76 5 and the field diaphragm 76 4 to improve intensity If using Hg or Xe gas discharge lamps an addi tional increase in intensity can be achieved by assembling the illumination telescope b...

Страница 62: ...ifications from 2 5x to 40x Turn a 10x objective into the light path and focus with the coarse and fine drive Close the aperture diaphragm until you achieve the desired image contrast Brightfield illu...

Страница 63: ...s changed The field diaphragm protects the specimen from unnecessary warming and enhances the image contrast It is therefore only opened wide enough to illuminate the observed or photographed field Su...

Страница 64: ...with 5x objective 0 01 mm 10 m with 10x objective 0 005 mm 5 m with 20x objective 0 002 mm 2 m with 50x objective 0 001 mm 1 m with 100x objective For the exact measurement of scale intervals for dif...

Страница 65: ...tube The lamp mount can be adjusted by a vertical distance of about 2mm by applying keys from underneath 87 9 Looking through the eyepiece adjust the height of the lamp until you obtain the brightest...

Страница 66: ...ay screw the macro device to the reflection optics with coupling ring 89 4 An image cannot be obtained without the reflec tion optics Like the slide overlay device the macro overlay only works in the...

Страница 67: ...a ted with the scale divisions on the macrodual zoom The following factors have to be multiplied for this Magnification factor of the working distance scale 89 9 e g 0 11x Zoom factor scale 89 8 e g 1...

Страница 68: ...8 6 541 510 c mount adapter 0 63 x HC 17 5 12 7 9 5 541 537 c mount adapter 0 5 x HC 16 12 541 511 c mount adapter 0 35 x HC 17 1 541 512 c mount adapter 4 x HC 4 2 8 2 1 5 without zoom magnification...

Страница 69: ...30 Leica MPS 60 Leica DMLD To adapt the microscope camera systems the following photoeyepieces and eyepiece adapter tubes are required Eyepiece HC 8x 20 PHOTO Eyepiece HC 10x 16 PHOTO Eyepiece HC 12 5...

Страница 70: ...ics absolutely clean Dust optics Remove any dust from glass surfaces with a fine dry grease free artists hair brush or by blowing with a bellows ball or by vacuum suction Any remaining dirt can be rem...

Страница 71: ...er M 25 Objective nosepiece 512 027 Screw cover M 32 BD objective nosepiece Spare eyecups glare protection for HC PLAN eyepiece 021 500 017 005 Eyecup HC PLAN 10x 25 eyepiece 021 264 520 018 Eyecup HC...

Страница 72: ...ean Union This declaration will cease to be valid if the instrument is modified without our consent Product name DM IRM Instrument type Light microscope Instrument no 090 133 701 to 706 EU directives...

Страница 73: ...64 41 29 25 99 http www leica com Leica Microsystems Wetzlar GmbH Ernst Leitz Strasse 35578 Wetzlar Tel 0 64 41 29 0 Fax 0 64 41 29 25 99 Printed on chlorine free bleached paper Order nos of the edit...

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