Leica BIOSYSTEMS 1404723512, Инструкция по эксплуатации, Страница: 27 / 42

27 VT1000 S
Operation
5
Warning
The blade must fit tightly against the entire length of the inner limit stop of the knife holder.
The blade must be clamped parallel to the front edge of both knife holder clamping jaws.
Fig. 25
1
2
• Fix the knife holder ( → Fig. 25- 1 ) with the knife holder clamping
screw (
→
Fig. 25- 2 ) .
• Use the REV/FORW rocker button to place the blade edge right
behind the rear edge (from user's view) of the specimen.
• Pull the
UP/DOWN
rocker button into the UP direction and keep it
in the UP position until the specimen surface is shortly below the
level of the blade edge (see arrow
( → Fig. 26- 1 ) ).
Fig. 26
1
• Select sectioning speed and sectioning frequency with the rotary
knobs SPEED and FREQ .
• Use the +/- button to select a sectioning thickness for trimming.
• Select a sectioning range appropriate to the size of the specimen
with the
SECTIONING WINDOW
button.
• Switch the
SINGLE/CONT
button to
CONT
.
Push the
START/STOP
button.
The instrument will now trim the specimen at the selected
trimming thickness until you push the
START/STOP button once
more.
• Once you have reached the desired specimen plane for
sectioning, use the
+/-
button to select the desired thickness for
sectioning.
Fig. 27
1
2
3
• For sectioning proceed as follows:
• Select the desired section thickness via the
+/- button.
• Switch the SINGLE/CONT button to SINGLE .
• Push the START/STOP button.
The instrument will now produce a section ( → Fig. 27- 1 ) . When
the section is finished, the knife will automatically stop at the rear
end position behind the specimen (from the user's view).
• Pick up the section as shown on the left using a brush
( → Fig. 27- 2 ) to mount it on a glass slide ( → Fig. 27- 3 ) .