LaMotte SMART3, Руководство оператора

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18 SMART3 Colorimeter 09.16
PREPARING DILUTE STANDARD SOLUTIONS
Standard solutions should be prepared to create a calibration curve. Standard solutions
can be prepared by diluting a known concentrated standard by specified amounts. A
chart or computer spreadsheet can be created to determine the proper dilutions. Use
volumetric flasks and volumetric pipets for all dilutions.
6. In Column A – Record the maximum concentration of test as determined by the
range and path length.
7. In Column B – Record the percent of the maximum concentration the standard
solution will be.
8. In Column C – Calculate the final concentration of the diluted standard solutions
by multiplying the maximum concentration (In Column A) by the % of maximum
concentration divided by 100. (C = A x B/100).
9. In Column D – Record the final volume of the diluted sample (i.e. volume of
volumetric flask).
10. In Column E – Record the concentration of the original standard.
11. In Column F – Calculate the milliliters of original standard required
(F = (C x
D
/
E
)).
A sample chart appears below:
A B C =
A x B /
100
D E F =
C x D /
E
Maximum
concentration
of test
% of Maximum
concentration
Final
concentration
of Diluted
Standard
Volume of
Standard
Concentration
of Original
Standard
mL of
Original
Standard
Required
10.0 ppm 90 9.0 ppm 100 mL 1000 ppm 0.90 mL
10.0 ppm 70 7.0 ppm 100 mL 1000 ppm 0.70 mL
10.0 ppm 50 5.0 ppm 100 mL 1000 ppm 0.50 mL
10.0 ppm 30 3.0 ppm 100 mL 1000 ppm 0.30 mL
10.0 ppm 10 1.0 ppm 100 mL 1000 ppm 0.10 mL
10.0 ppm 0 0 ppm 100 mL 1000 ppm 0 mL