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Algorithm Settings:
Adaptive Threshold
•
Window Size.
This is the area (in pixels) around a pixel used to calculate the threshold baseline.
Setting this to your average particle size is a good starting point.
•
Preblur Image.
When selected, an algorithm is used to filter out image noise. Keep this option
enabled for best results, unless the images have little noise.
•
Threshold Start and Stop Values.
This sets the contrast differential threshold between the
object and background. A low setting would include objects with lower contrast differential,
where a higher level would filter out objects with low differential. This allows absolute
threshold values to vary in relation to lighting un-evenness or out-of-focus background in an
image. For best results, set
the “
start
”
threshold equal to or greater than
the “
stop
”
threshold.
Adjust the thresholds up or down until only real particles are counted. NOTE: For fastest
processing, set the starting threshold equal to the stop threshold.
Particle filters
•
Minimum Distance.
This setting is used to filter particles that are closely spaced. Two
particles spaces closer than the set minimum distance will be counted as one particle.
•
Image areas.
This setting is used to filter particles by size. Only particles sized (in pixels)
between the set min and max values will be counted.
•
Circularity.
This setting is used to filter particles by shape. A circular particle will have a
circularity value close to 100%, if the particle is rod-like or otherwise not circular this
ratio is lower.
•
I ratio
and
Convexity
are currently not enabled.
