Labnet BioPette PLUS BPP2, Инструкция пользователя, Страница: 8 / 11

9
• When pipetting acids or corrosive solutions which emit
vapors, it is recommended to disassemble the shaft
and to rinse the piston and seal with distilled water after
finishing the pipetting operation.
8 - RECALIBRATION
Labnet
pipettes are calibrated by gravimetric method,
using
Labnet
tips and distilled water, at the temperature
20±1°C, according to EN ISO 8655 standard.
If during pipette operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in section 1, the pipette recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been fulfilled
during error determination:
• the ambient temperature, and the temperature of the
pipette, tips and water was identical
• the density of the liquid used is close to that of distilled
water
• a balance with appropriate sensitivity has been used.
• mg/μl conversion factor has been taken into account
• the requirements given in sections 3 and 7 have been
fulfilled
If the above conditions are satisfied and the accuracy
error for selected volume given in section 1 exceeds the
permissible value, the pipette recalibration procedure
should be carried out.
The recalibration can be performed within one full
turn of the key to the right or to the left only.
Recalibration conditions:
• Ambient temperature and the temperature of the pipet-
te, tips and liquid should be within the range 20-25°C
and stabilized during weighing within ±0.5°C
• Measurements should be conducted using distilled water
• Balance sensitivity should be suitable for the volume to
be controlled
10
Recalibration procedure:
• Set the dose volume depending on the pipette volume
according to the following table:
• Perform 5 aspirations, weigh each one and calculate
the average value of the aspirations
• Calculate average aspirated volume in μl multiplying
the average aspiration amount [mg] by the distilled
water density coefficient [μl/mg], which depends on
temperature and pressure according to the following
table:
If the average aspirated volume exceeds the permissible
value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A),
Warning: The pipetting pushbutton consists of 2 parts:
the knob (Fig. 1A2) and the pushbutton (Fig. 1A1). After
removal of the pushbutton, both parts are separated.
• Holding the volume adjustment knob to protect it
against rotation, insert the calibration key into the cuts
of the calibration screw, (Fig. 4B),
BPP2
BPP10
BPP20
BPP100
BPP200
BPP1000
BPP5000
BPP10000
0.1 - 2
0.5 - 10
2 - 20
10 - 100
20 - 200
100 - 1000
1000 - 5000
1000 - 10000
0.2
0.5
2
10
20
100
1000
1000
0.176 - 0.224
0.48 - 0.52
1.94 - 2.06
9.84 - 10.16
19.76 - 20.24
98.4-101.6
994 - 1006
975 - 1025
0.06
0.33
0.63
2.50
6.30
25.00
125.00
250.00
Model
Range
of the pipette
volumes
[μl]
Preset
volume
[μl]
Permissible
volumes
[μl]
Volume change
Δ
V for full turn
of the calibration
key [μl]
(24 increments)
Volume checked [μl]
0.1 - 10
10 - 100
> 100
Balance sensitivity [mg]
≤
0.001
≤
0.01
≤
0.1
Temperature [°C]
Pressure [kPa]
95.0 101.3 105.0
20 1.0028 1.0029 1.0029
21 1.0030 1.0031 1.0031
22 1.0032 1.0033 1.0033
23 1.0034 1.0035 1.0036
24 1.0037 1.0038 1.0038
25 1.0039 1.0040 1.0040