5
Overview
KNAUER
Degasser 20DG User Manual V7632A, Version 1.1
Overview
HPLC
High pressure liquid chromatography (HPLC) is a method for
separating substance mixtures, determining substances, and
measuring their concentration.
BlueShadow
Degasser 20DG
Dissolved gases in the solvent can cause bubbles in pumps and
the detector. Good chromatographic separation therefore
requires degassing of the solvent. The analytical 2-channel
Degasser 20DG is equipped with two degassing chambers and
can thus degas two solvents simultaneously.
Degassing Principle
As the solvent flows through the short Teflon AF
®
tubes, the dis-
solved gases are removed from the solvent by means of a vac-
uum on the other side of the membrane. An integrated vacuum
pump maintains a constant vacuum.
The special pump design makes it possible to let a small blast of
air flow around the pump head, to remove solvent fumes which
could escape from the vacuum chamber.
Degassing Principle of the Teflon AF
®
Mem-
brane
Structural properties of the Teflon AF membrane accelerate
the degassing process.
Due to the molecular structure of the Teflon AF membrane,
only a very small surface is required for degassing the solvent.
Thus the risk of carryover during a solvent change is reduced.
The solvophobic and hydrophobic properties of Teflon AF
reduces the risk of contamination between the individual sol-
vent chambers.
Room ventilation,
A/C system,
sunlight
Always use the device in rooms that are well-ventilated, and are
preferably equipped with an air-conditioning system. When set-
ting up the device at the installation location, make sure that it is
protected against direct sunlight.
Fig. 1
Degassing principle
