29
Operation
BlueShadow Pump 80P Instructions V7685
Flush the pump in the following cases:
At initial startup to eliminate air bubbles in hoses and capillaries.
When changing solvents.
After using buffer solutions to eliminate salt residues.
Before turning off, if you do not plan to restart the device within
shortly.
The installation has been completed.
The capillaries and tubings have been connected.
The pump has been switched on and is in „flow mode“.
Syringe with Luer lock.
Use the purging solvent to be used in the following application.
Note:
The purging process may take a while during initial operation be-
cause the solvent tubes are filled with liquid for the first time.
Note:
If a buffer solution is used, pay attention to choosing a solvent for
purging in which the buffer solution is soluble.
Procedure
Process
Figure
1.
Open the venting screw
1
of
the pressure sensor.
2.
Connect the syringe with the
silicone tubing to the
outlet
2
.
3.
Press the purging button
to open the purging mode
and select respective gra-
dient and flow.
4.
With the syringe, extract fluid
through the purge port
2
.
5.
If the extracted fluid flows
continuously, stop suc-
tion and close the venting
screw
1
.
Fig. 16:
Venting screw of the
pres sure sensor
The purging process of the pump is limited to a maximum pressure of
5 MPa. If this value is exceeded during the purging process, the pump
switches off automatically. If you are using very small hoses and capilla-
ries, the pressure can be too high.
5.3.1 Piston backflushing
The pump is equipped with automatic piston backflushing. It increases
the service life of the seals and pistons, and removes contaminants from
the area behind the seals.
The piston backflushing function automatically flushes the rear piston
area of the pump head upon switch-on and in continuous mode.
When is purging
required?
Prerequisites
Tool
Functional principle
1
2
