Operating modes
Positions for column bypass / manual injection valve
16 Port Multiposition valve
Inject (2)
Load (1)
6 Port 2 Position valve
1
1
2
2
3
3
4
4
5
5
6
6
A
B
C
3
Sample loop selection valves / fractionation valve
1 2
3
4
16
A. Loading samples
1. Set the injection valve to Load (1) position.
2. Address the sample loop you want to load
(start with position 1) by switching the two sam-
ple loop selection valves.
3. Rinse the sample loop tubing with solvent. Op-
tionally empty tubing by injecting air.
4. Load the individual sample through the injec-
tion port of the injection valve with 3x excess of
the sample loop volume.
5. At the end of sample loop loading, switch the
two sample loop selection valves to the next
position. The loaded loop is now closed.
6. Rinse the tubing and valves with solvent. Optio-
nally empty tubing by injecting air.
7. Repeat procedure for all required sample
loops.
8. After sample loop loading: Switch the two
sample loop selection valves to waste (position
16 with short tubing) and rinse with solvent to
remove air.
Note:
For each loading, make sure that the
same position is set for both sample loop se-
lection valves. If both valves are not switched
simultaneously, the fl ow must be stopped.
Otherwise strong pressure spikes can occur
and may cause the pump to stop.
Note:
To prevent air bubbles to enter the
sample loop do not remove the syringe
before switching both sample loop selection
valves to next or washing position (position
16 with short tubing).
B. Equilibrating column
Before the sample cleanup the GPC column has to
be equilibrated with the desired eluent. Procedure:
1. Set the injection valve to Load position.
2. Set column bypass valve to Inject position to
integrate the column into the fl ow.
3. Switch fractionation valve to designated Waste
position.
Note:
It is suggested for the column equilibrating
step to use position 16 of the fractionation valve
as waste.
C. Running the GPC
After sample loops have been fi lled, the samples
are processed one after the other. Procedure:
1. Set the injection valve to Inject position.
2. Select the individual sample loops by setting
the two sample loop selection valves.
3. Set the column bypass valve to Inject position
to integrate the column into the fl ow.
4. Switch the fractionation valve to collect frac-
tions.
