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OBL-14-15-BA-e-1610 

 

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4.  Open the field diaphragm until it just disappears out  of the 

field of view. 
If necessary, simply re-centre using the centring screws on 
the condenser holder. 

 
 
 
 
 
 
 
5.   Use  the  aperture  diaphragm  of  the  condenser  to  find  the 

very best compromise between contrast and resolution for 
the  microscopic  image.  The  scale  divisions  on  the 
condenser  can  be  used  as  a  guideline.  Select  in 
accordance with the objective being used. 

 
 

The  view  in  the  tube  without  the  eyepiece  should  look 
something like the illustration on the right. 
The  diameter  of  the  aperture  diaphragm  which  is  then 
visible  should  make  up  approximately  2/3  of  the  pupil 
diameter. 

 

If the eyepiece should be removed, for checking, then please make sure that no 
dirt or dust falls into the tube. 

 
 
 
6.  It is possible to alter the brightness of the bulb using the 

dimmer

. The brightness 

is always controlled by the bulb brightness and not by the aperture diaphragm. 

 
 
 
7.  Possibly there is the need of re-adjusting the focus and x-y axis. 
 
 
 
8.  Observe the object. 
 
 
If another magnification is selected afterwards, then the Koehler illumination does not 
have to be reset from scratch, only the aperture diaphragm and field diaphragm need 
to be adjusted as required. 
 

 

Содержание OBL 145

Страница 1: ...gelei 1 D 72336 Balingen E Mail info kern sohn com Tel 49 0 7433 9933 0 Fax 49 0 7433 9933 149 Internet www kern sohn com User instructions Transmitted light phase contrast microscope KERN OBL 14 OBL...

Страница 2: ......

Страница 3: ...4 3 Eyepieces 10 4 4 Colour filter 10 4 5 PH condenser PH slide 11 5 Operation 11 5 1 Getting started 11 5 2 Pre focussing 12 5 3 Adjusting the interpupillary distance 13 5 4 Dioptre adjustment 13 5...

Страница 4: ...an additional risk of triggering an electric shock 1 2 Notes on the electrical system Before connecting to a mains power supply you must make sure that you are using the correct input voltage The inf...

Страница 5: ...hich are too high or too low vibrations dust or a high level of humidity The ideal temperature range is between 0 and 40 C and a relative humidity of 85 should not be exceeded The device should always...

Страница 6: ...as these are highly flammable substances You must therefore keep it away from naked flames and electrical devices which can be switched on and off and only use it in well ventilated rooms However orga...

Страница 7: ...OBL 14 15 BA e 1610 6 2 Nomenclature...

Страница 8: ...7 OBL 14 15 BA e 1610 Rear view Adjusting ring torque Carrying handle Main switch Power connection Coarse adjustment knob Fine adjustment knob Fuse Condenser Focus dial Adjusting ring torque...

Страница 9: ...ocular 6V 20W Halogen Transmitted light OBL 155 Infinity Trinocular 6V 20W Halogen Transmitted light Eyepieces WF 10x 20 mm Objectives 4x PH10x PH40x 100x Product dimensions 395x200x380 mm Packaging d...

Страница 10: ...9 OBL 14 15 BA e 1610...

Страница 11: ...n such a way that if you turn the nosepiece clockwise the objective with the next higher magnification appears When the objectives need to be dismounted you should always make sure that you do not tou...

Страница 12: ...erted into the appropriate point on the front side of the condenser For further information on this please see 5 9 Phase contrast unit 5 Operation 5 1 Getting started The very first step is to establi...

Страница 13: ...s soon as an image is recognisable no matter how sharp then you should only adjust the focus using the fine adjustment knob Adjusting the torque of the coarse and fine adjustment knob Next to the left...

Страница 14: ...ircular image then the interpupillary distance is set correctly 5 4 Dioptre adjustment The eye strengths of each eye of the microscope user can often be slightly different which in daily life has no c...

Страница 15: ...l objectives you should use the silver ring above the objectives see illustration When rotating the nosepiece you must always make sure that the objective which is about to be positioned in the beam p...

Страница 16: ...ion However this is regarded as Fixed Koehler since the condenser is pre centred When adjusting the Koehler lighting for the first time you must first select the lowest possible objective magnificatio...

Страница 17: ...e illustration on the right The diameter of the aperture diaphragm which is then visible should make up approximately 2 3 of the pupil diameter If the eyepiece should be removed for checking then plea...

Страница 18: ...lasses symbol on the side They are also marked in the item description by an additional H example HSWF 10x 23 mm When fitting the eye cups make sure that the dioptre setting is not moved We would ther...

Страница 19: ...slide 2 Lower the specimen stage and position the 100x objective in the beam path 3 Bring the specimen stage or object slide to the objective very slowly until there is slight contact 4 Observe the ob...

Страница 20: ...simple PH condenser a PH objective with a specific magnification 10x 20x 40x or 100x a PH slider which is adapted to the lens being used a centring telescope and a green filter The standard outfit of...

Страница 21: ...the ring in the slider however can be Before starting the phase contrast application you have to check if this alignment is given For this you must install the centring telescope to the tube connecto...

Страница 22: ...der is on the underside of the device It can be opened by undoing the screws on the holder see left illustration We recommend that here you should also test again to check that heat is no longer being...

Страница 23: ...ead then a suitable adapter must be fitted In general there are two C mount adapters available for this 1x and 0 47x magnification see Chapter 3 Features After fitting one of these adapters it can be...

Страница 24: ...tly centred The condenser is too low The field of view is dark or is not correctly illuminated The objective is not positioned correctly on the beam path The selector switch for the beam path is betwe...

Страница 25: ...s Dirt dust on the front lens of the condenser Dirt dust on the object One side of the image is blurred The stage was not correctly fitted The objective is not positioned correctly on the beam path Th...

Страница 26: ...ly materials which you can dispose of at your local recycling centre Disposal of the storage box and device must be carried out by the operator in accordance with all national or regional laws in forc...

Страница 27: ..._______________________________________________________ __________________________________________________________ __________________________________________________________ __________________________...

Страница 28: ...27 OBL 14 15 BA e 1610...

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