Version 2017-01-19 for GI use
Press the HT knob
1
under the plastic cover (it lights up) and check that the green
light is ready. Beam current (
3
) rises to 040. Press the FIL knob
2
on. Also here a
green ready light goes up and the current
3
rises from 40 until about 60µA.
Open the iTEM software (click on icon on desktop)
Press Intelligent exposure (pink cross)
St
art imaging at low magnification. For magnification below 800x press the “
LOW
MAG
” knob in the Magnification Selector panel. The lower diaphragm handle
f
should
be switched to the right. For still low magnifications, but above 800x, use
MAG 1
and
turn diaphragm
f
to the left. To modify the magnification factor, use switch
4. MAG 2
is for pre-set magnifications. (If possible, use standard magnification steps to better
compare images).
XY navigation with
14
and
15
Spread the bundle as evenly as possible just over the field of view using the
brightness knob
12.
Use the Shift
10
and Shift
13
knobs (right and left panel) to center the bundle. (make
the bundle small to better judge the center. In general, when brightness needs to be
dimmed, it is recommended to use the domain that can be reached by turning the
know clockwise
Focus using
5, 6, 7.
Option 16x (
5
) is for a very coarse focusing. It can be employed
with both the macro (
6
coarse) and micrometer (fine
7
) knob. Without 16x a finer
tuning can be achieved. (In summary, there are four grades of focusing)
To further improve the focusing, use the wobbler function x and y (
8
and
9
) and focus
until the image is stable on the screen