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2. Using the focusing knob, focus the sample using the highest magnification
strength. If the sample cannot be brought into focus, adjust the height of the
microscope along the column. Remember to tighten the locking screw and
support collar after adjusting the height of the microscope.
3. Turn the zoom to the highest magnification. Adjust the focusing knob until a
clear and sharp image is obtained.
4. Turn the zoom to the lowest magnification. Adjust the right eyepiece diopter
until the image seen through the right eyepiece is clear and sharp.
5. Repeat the procedure for the left eyepiece. Next, check the image focus for
the entire zoom range; it should now be perfectly parfocal.
6.5 Magnification and Working Distance
1. Select the desired magnification strength by adjusting the zoom knob. Change
the optional eyepieces and/ or add an auxiliary objectives, for other range of
magnification.
2. Total magnification used can be calculated using the following equation:
Total magnification = Eyepiece magnification x Zoom magnification x Objective
lens magnification
6.0 Using the Microscope
