Adjusting the Collection Schedule
Once the appropriate column has been selected and mounted to the AFC tower, the
collection schedule must be set.
1.
From the home screen, you can adjust the count, volume, and buffer volume for
collection. Press
-/+
and follow the on screen prompts to save your parameters.
Setting up the Carousel
1.
Load the collection tubes onto the carousel with the lids facing inwards. Ensure
you have loaded enough tubes for your collection protocol.
2.
Gently place the carousel onto the carousel plate. Ensure that the carousel alignment
hole engages with the raised carousel alignment pin on the carousel plate.
Remember to label your tubes before placing them into the AFC carousel.
Flushing the Column
1.
The next screen will ask you to mount the column reservoir. Note: If using a qEV
single, a column reservoir is not needed.
2.
Press
OK
to continue and follow the on screen prompts. It is recommended to flush
your column with fresh, filtered PBS buffer.
Sample Loading and Initiating Collection
1.
The AFC will ask you to have sufficient buffer available to run the column and to
load the sample.
2.
Ensure that there is no residual buffer left on the top of the column, remove any
excess with a pipette. Now add your sample.
It is important to only load the sample at this point. Adding buffer at the same
time or before the sample has fully entered the frit of the column will result in
incorrect sample loading.
3.
Press
OK
to continue.The column will start running and the carousel will move to
the buffer collection position. The lighting will change to
red
to indicate that the
collection has started.
4.
When the sample has fully entered the upper frit of the column, top up the column/
reservoir with the correct amount of buffer to resume collection of the buffer volume.
5.
When the buffer volume has been collected, the carousel will move to position 1,
collect the specified volume and then progressively move to collect each successive
individual fraction. If more collection tubes are required than a fully loaded carousel,
the user will be instructed when to remove the cover and carousel, replace the tube
positions with fresh tubes and continue the run.
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