Concentrating Pipette Select Quick Start Guide
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Installation of the Instrument Components:
1.
Plug the 12V DC power supply into an available wall receptacle (110 -240 V AC 50/60 Hz 1.0 Amp) and then into the
12V
DC Jack
on the side of the instrument.
NOTE: Only use the power supply provided with the instrument. Use of any other
power supply, including those from the previous Concentrating Pipette (CP-150), may damage the instrument and will
void the warranty.
2.
Attach the
Permeate Line
to the
Permeate Line Port
using the quick connect fitting and insert the other end of the
permeate tube into a waste container and secure using the supplied
Permeate Line Clip
.
3.
Insert the provided
Reusable Storage Fluid Adapter
into a bottle of
Storage Fluid
and stow in the
Storage Fluid Bracket
on the right side of the instrument. The
Reusable Maintenance Tip
is also stowed using this
Bracket
.
The Start-Up Procedure:
This procedure should be used the first time a new instrument is powered on
and each time the instrument is powered up after
previously going through the Shut-Down procedure (the Shut-Down Procedure is described below).
1.
Flip the
Power Switch
to turn the instrument on – it will take approximately 5 seconds for the unit to power up.
2.
Press Enter to advance from the Welcome Screen to the Main Menu.
3.
Select
Maintenance>Start-up
4.
Follow the menu prompts to be guided through the Start-Up Procedure.
5.
The instrument will now return you to the Run Protocol screen and is now ready for use.
Selecting a Protocol:
1.
On the Run Protocol screen the selected Protocol is displayed in all-caps above Start Run.
2.
If a different Protocol is desired use the Arrow and Enter keys to select the displayed Protocol. This will take you to the
Protocol Setup screen.
3.
Select
Select Protocol
and then use the Arrow keys to select the desired Protocol.
4.
You will now be returned to the Run Protocol screen and the newly selected Protocol name will be displayed.
Performing a Sample Concentration Run:
1.
Select the CPT of your choice (See Table 2.1 Concentrating Pipette Tip Selection Guide in the User Guide) and insert the
CPT into the Tip Interface Port.
2.
Check that the Protocol listed on the Run Protocol screen matches the CPT type you are using. If not, follow the
procedure provided above for Selecting a Protocol.
3.
Place your sample container on the sample platform and lower the CPT into the sample until it contacts the bottom of
the container. Select
Start Run
. The vacuum pump will start to draw the sample through the CPT. When the entire
sample has been processed, the instrument will conclude the run automatically. The menu will display the Run
Complete screen which shows the processing time, and gives the option to Elute, Wash, or Return to the Main Menu.
4.
Select
Elute
. Follow the menu prompts to be guided through the Elute Procedure. Additional Elutions may be performed
by selecting "Elute Again".
5.
Remove the used CPT by pushing your thumb up against the bottom of the
Fluidics Head
while pulling the CPT down
with your hand.
Creating a Custom Protocol:
1.
From the Select Protocol menu select FLAT (or the current protocol). This will advance you to the Protocol Setup menu.
2.
Select
Create Protocol
. You will be prompted to create a name for your protocol. Use the Arrow Keys to cycle through
numerical and alpha characters sequentially from 1-9 followed by A-Z. To select a character, press Enter. Advance to the
next menu by pressing and holding Enter.
3.
You will be taken to a menu with the created protocol name at the top and standard or advanced settings for the
protocol below. Standard settings will be shown if
Protocol: Std
has been previously selected in the System Settings and
Advanced settings will be shown if
Protocol: Adv
was selected. System Settings may be accessed through the Main
Menu. Descriptions of the standard and advanced settings are provided in Section 8 of the User Guide.
The Shut-Down Procedure:
This procedure should be used at the end of each day of sample concentration runs.
1.
From the Main Menu select
Maintenance>Start-up
2.
Follow the menu prompts to be guided through the Shut-Down Procedure.
