ibidi ibidi Pump System, Инструкция по эксплуатации

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ibidi Pump System
7.1.2 Flow Calibration Of Two or More Fluidic Units
To calibrate a system running with more than one Fluidic Unit, we recommend measuring the flow
rate of each Fluidic Unit separately. The flow calibration procedure is outlined in Section
6.6 .
Prepare your Fluidic Units and Perfusion Sets as follows:
1. Install the system as described in Section 7.1.1 .
2. Pinch off the tubing of all Perfusion Sets that are not to be calibrated (3 if you are working with
4 Fluidic Units). It is sufficient to pinch one of the tubing branches, as shown in the pinch test
(Figure
29 ).
3. Expel the air bubbles from the remaining Perfusion Set by means of the manual control panel
in the PumpControl software ( 6.4 ).
4. Perform the Pinch Test (Section 6.5 ).
5. Equilibrate the liquid levels.
6. Measure the flow rate as described in Section 6.6 .
7. Note the flow rate in your documentation.
8. Perform step 2 to 7 for each Fluidic Unit.
9. Finally, calculate a mean flow rate for all Fluidic Units. For reproducible results, the variation
of the measured flow rates should not be higher than 10% between the Fluidic Units.
10. Insert the mean value in the box ”measured flow rate” as explained in Section 6.6.3 .
7.2 Calibration of the Flow Rate with Several Slides in Serial Connection
A calibration before starting an experiment with serially connected slides is mandatory. When con-
necting several slides to one Fluidic Unit, flow resistance is increased, leading to decreased flow rate
with significant differences to the standard setup.
The calibration procedure is the same as described in Section
6.6 . See Application Note 25 “Serial
Connection of Flow Chambers” for instructions on how to connect several slides serially.
Note!
When connecting several slides serially the flow rate (and thus the shear stress) is the same in all
slides!
Version 2.6 (May 12, 2022) 52 Instruction Manual