ibidi Pump System
Figure 30: The correlations among pressure, flow rate and shear stress in the ibidi Pump System.
Because of manufacturing tolerances, setup variations, and temperature fluctuations, the values for
each Perfusion Set can differ from the values given in the software. To obtain the required experi-
mental flow rate, we recommend performing a system fine calibration.
6.6.1
Connect the Calibration Slide to the Perfusion Set
For calibrating the system, a sterile µ-Slide (the same type as in your experiment) without cells must
be connected to the Perfusion Set.
1. Check the tubing for air bubbles and use the hose clip to clamp both tubing arms of the Perfu-
sion Set tubes directly beneath the valve (Figure
Figure 31: Clamp the tubing before opening the Female Luer Coupler of the Perfusion Set.
2. Before connecting the Perfusion Set to the µ-Slide, the reservoirs of the slide must be filled to
the top to avoid trapping bubbles in the reservoir (Figure
). For a detailed description, refer
to
Application Note 13, “Endothelial Cells Under Perfusion”
3. Remove the hose clip to start the flow rate measurement.
Version 2.6 (May 12, 2022)
44
Instruction Manual
