ibidi Heating System, Multi-Well Plates, K-Frame
Note!
The temperature of the Heated Lid must be set several degrees warmer than the temperature of
the Heated Plate!
When changing the incubation temperature, the Heated Glass Bottom must always be set to
1°C lower than the temperature of the Heated Plate, e.g. if the Heated Plate is set to 39°C, the
Heated Glass Bottom must be set to 38°C. If the Heated Glass Bottom isn’t set 1°C lower, the
temperature distribution in the sample may not achieved the specified values.
The temperature adjustment must be repeated from time to time (at least once a year), especially if
one of the following conditions has been changed:
• Room temperature
• Air conditioning
• Chamber type or objective lens
• Humidification and gas flow
• Use of an XL-Incubator
• Use of an Objective Heater
5.5
Sample Preparation
• Prepare the cells according to your protocol and place the multi-well plate in the Heated Plate
mounted on the microscope stage.
• Close the Heated Lid.
• Let the temperature of the system equilibrate for a minimum of 30 min before you start your
experiments.
Note!
Working with high aperture objectives might limit the observation area due to their large diam-
eter. In this case ibidi recommends leaving out the outermost rows of wells to avoid the risk of
objective collision with the Heated Plate.
Version 2.0 (March 22, 2019)
24
Instruction Manual
