
UV transilluminator function
The Hoefer
™
MacroVue
™
variable UV
transilluminator provides a uniform and intense
source of 302 nm ultraviolet light to back-
illuminate transparent fluorescent materials.
Nucleic acids stained with fluorescent dyes
such as ethidium bromide and acridine orange
can be detected under UV illumination. This
visualization method is especially suitable for
double-stranded nucleic acids, but is less sensitive
for visualizing single-stranded nucleic acids.
The MacroVue UV-25 filter surface is 21
×
26 cm. The transilluminator can be converted
from 302 nm (medium wavelength) to 254 nm
(short wavelength) by replacing the UV lamps as
described on page 6.
•
p1
Fig 1. MacroVue UV-25
Transilluminator main
components.
Variable intensity dial
Illumination
area (filter)
Mains power
switch
Hinged UV-blocking
safety cover