Start Processing
20.
Turn on the AP 720S. After initializing, the [Main Menu] screen
will be displayed.
21.
Select the panel type (Panel 20 or Panel 36). Press “Next” and
move to the [Check] screen.
22.
Ensure there is sufficient Antibody Reagent in the Reagent
Reservoir, sufficient Wash Buffer in the Wash Buffer Bottle, and
sufficient Deionized Water in the Deionized Water Bottle, and
check that the Waste Bottle is empty.
23.
Press “Start” to begin automated processing. (There are 8
processes in all to complete the Auto-Run).
Load Photoreagent Mixture
24.
At the end of Process #7, the [Change Photo Rgt] will appear,
the AP 720S will pause and an alarm will sound. Press “Alarm Off”.
25.
Prepare Photoreagent Mixture by mixing an equal amount of
Photoreagent AB and CD. Note: Allow Photoreagent AB and CD
to come to room temperature prior to mixing. Once mixed, the
Photoreagent should be used immediately for best results.
26.
Fill the Photoreagent Reservoir (RED line) with the required
amount of Photoreagent Mixture as listed in the Instruction
Manual.
27.
Open the door. Remove the Antibody Reservoir and load the
Photoreagent Reservoir in the Sample / Reagent Rack. Close
the door.
Continue Processing
28.
Press “Continue” on the [Change Photo Rgt] screen.
29.
Press “Yes” on the [Check] screen to start Process #8
(Photoreagent Aspiration).
30.
As soon as the first pette is filled with Photoreagent Mixture,
set a timer for 10 minutes.
31.
At the end of Process #8, an alarm will sound. Press “Alarm Off”
on [Process End] screen.
Unload and Read Pettes
32.
Open the door, remove the Pette Rack from the instrument and
set on the Pette Stand.
33.
Insert a black top plug into each Pette.
34.
Unload the pettes one by one from the Pette Rack.
35.
Remove the Pette Tip from each Pette.
36.
Insert a white bottom plug into each Pette.
37.
Load the Pettes in order into the Pette Cassette Tray.
38.
When the timer reads 10 minutes load the tray in the CLA-1
Luminometer and read the test results.
Completion of Operation
39.
Remove the Sample / Reagent Rack and discard all used sample
cups.
40.
To run a second set of pettes immediately following the first set,
press “End” on the [Process End] screen to return to the [Main
Menu] screen.
41.
Alternatively, to prepare for shutdown, press “Rinse” on the
[Process End] screen.
42.
Unscrew the caps on the Wash Buffer and Deionized Water
bottles. Pull out the tube from the Wash Buffer Bottle and place
it in the Deionized Water Bottle along with the Deionized Water
tube. Press “Ok” in the [Rinse] screen.
43.
On completion of the rinse remove the Wash Buffer and Deionized
Water tubes from the Deionized Water bottle. Place Wash Buffer
and Deionized Water tube on a clean surface. Press “Ok” on the
[Drain] screen.
44.
After the drain function is finished the [Main Menu] screen
appears. Turn off instrument.
45.
Rinse Reagent Reservoirs with deionized water.
Doc. No. 0822 - ENG Rev. 00
www.hcdiagnostics.com
QUICK START
TM
AP 720S Semi-Automated Instrument (continued)
[Main Menu]
[Panel Type]
[Check]
[Change Photo Rgt]
Elapsed Time 0:0:15
[Check]
Add Photo Rgt?
[Process End]
Elapsed Time 0:0:15
Antibody,
Wash Buffer,
Water.Waste
[Panel 36]
Assay
Panel 36
Next
Back
Start
Alarm Off
Yes
Alarm Off
Rinse
End
No
Continue
[Rinse]
Please change tube
to Water Bottle.
[Drain]
Please pulll out tube
from Water Bottle.
Back
Ok
End
Ok
36
20
Maintenance
07/ 2/23
14:25:21
