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Publication 5390-001 Revision B

Har

ard Appar

atus Model 33

win Syr

inge Pump User's Man

ual

Specifications

Harvard Pump 33 Specifications

Accuracy

±0.35%

Reproducibility

±0.1%

Type

Dual Syringe Infusion/Withdrawal Parallel/Reciprocal

Size, L x W x H

(286 x 311 x 152 mm) 11.25 x 12.25 x 6 in

Weight

(6.8 kg) 15 lbs

Power

115/230 VAC, 50-60 Hz via selector switch, 45 W

Fuse

1 A (115 V), 0.5 A (220 V)

Leakage to Ground

Typically < 10 µA

Ground Resistance

Typically < 0.06

Ω

Voltage Operating Range

95-130 VAC, 220-260 VAC

Drive Motors

2 motors, each 0.9 degree steppers

Motor Drive Circuitry

Microprocessor controlled from 1/2 to 1/4 microstepping

Timing Belt Drive

2:1

Lead Screw Pitch

24 threads/inch

Motor Steps per One

1600 at 1/2 stepping or 3200 at 1/4 stepping

Revolution of Lead Screw

Step Rate:

Minimum

27.3 sec/step

Maximum

416.7 µsec/step

Pusher Advance per Step:

Minimum

0.330729 µm

Pusher Travel Rate:

Minimum

0.726699 µm /min

Maximum

95.25 mm/min

Dynamic Speed Range

131,072 to 1

Force

40 lbs max.

Pressure

99.5 PSI max. with 20 ml stainless steel syringe

Calibration

Enter syringe diameter, any size, any make, up to 50 mm
diameter

Syringe Size

10 µl (min.), 50 ml (max.)

Flow Rate Range:

Minimum

0.0073 µl/hr (10 µl syringe)

Maximum

53.346 ml/min (50 ml syringe)

Display

5 digits plus 14 indicator LEDs

RS-232 Interface

Chained dual bidirectional ports

Non-Volatile Memory

Storage of all settings

Содержание 33

Страница 1: ...Syringe Pump Model 33 MA1 55 3333 Model 33 Twin Syringe Pump User s Manual Publication 5390 001 Revision B ...

Страница 2: ...mping Directions 10 Running the Pump 10 Single Syringe Operation 10 Valve Control Box 11 Pinch Valve Connectors 12 13 External Control and Interfaces 14 15 Attaching Configuring a Pump Chain 14 TTL Devices 15 Foot Switch Input 15 Pump Chain Commands 16 20 Pump Commands and Responses 17 Pump Chain Error Messages 20 Appendices 21 27 A Syringe Diameters in mm 21 B Nominal Min Max Flow Rates 22 C Comm...

Страница 3: ...ES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 or outside ...

Страница 4: ...or controlled from 1 2 to 1 4 microstepping Timing Belt Drive 2 1 Lead Screw Pitch 24 threads inch Motor Steps per One 1600 at 1 2 stepping or 3200 at 1 4 stepping Revolution of Lead Screw Step Rate Minimum 27 3 sec step Maximum 416 7 µsec step Pusher Advance per Step Minimum 0 330729 µm Pusher Travel Rate Minimum 0 726699 µm min Maximum 95 25 mm min Dynamic Speed Range 131 072 to 1 Force 40 lbs m...

Страница 5: ...e used to control an external valve for refilling and provide an out put to indicate whether or not the pump is running AdditionalTTL pins are avail able for general use RS 232 Multiple pumps can be chained together and remotely controlled from a computer or any device communicating via RS 232 Valves Models MA1 55 3334 and MA1 55 3335 include a valve assembly which allows auto matic selection of i...

Страница 6: ...other than 115VAC 60 Hz is being used the plug must be cut off and an appropriate plug installed observing the polarity of the interna tional line cord used Brown high Blue neutral Green ground 3 Turn on main power switch located directly above the line cord on the rear panel The display will illuminate indicating that the power connections are cor rect The flashing display indicates that power ha...

Страница 7: ...her block from the leadscrew turn the knob on the front of the block 1 until the pin in the knob slips into the hole in the block 4 The syringe clamp locking screw on the right side of the syringe block 4 should be loosened and the clamp rotated to the side 5 Place the syringe barrel on the syringe holder block and move the pusher block to accommodate the plunger 6 Make sure the syringe barrel fla...

Страница 8: ...ue by pressing the corresponding key or when the pump is alerting the user to a problem Such problems are a value out of range oor or the pump stalling StALL The 14 LED indicators are divided into three sections Pump mode and state syringe directions and units of value being displayed See Figure 2 The LED indicators are as follows Pumping Mode and State LEDs Meaning Auto Stop Pump is in Auto Stop ...

Страница 9: ...its when setting Rate 2 Displays sets current rate of syringe 2 Scrolls through rate units when setting Key only valid in Proportional mode Diameter 1 Displays sets current diameter of syringe 1 Also diameter of syringe 2 if current mode is not Proportional Diameter 2 Displays sets current diameter of syringe 2 Key valid in Proportional mode 1 Used to display and set current Pump Chain address and...

Страница 10: ...ncluding up to four decimal places Entering more than five digits will clear the previous five digits in the display Press the green ENTER key when the desired data value is displayed If the data value entered is outside the pump s operating parameters the display will read oor meaning Out of Range Pressing any key will restore the display with the original data value Enter another data value with...

Страница 11: ...sired mode either Auto Stop Proportional or Continuous mode Auto Stop Both syringes pump according to DIAMETER 1 and RATE 1 until syringe 1 reaches the limit switch when pump will stop Proportional Each syringe pumps independently syringe 1 according to DIAMETER 1 and RATE 1 syringe 2 according to DIAMETER 2 and RATE 2 When syringe 1 reaches limit switch pump stops Continuous Both syringes pump ac...

Страница 12: ...gs Setting up Valve Set up consists of 1 Pump to Valve Electrical Connections The cable fitted with a male 3 pin connector must be connected to the female connector on the rear of the Model 33 The Model 33 should be turned off when making breaking this connection 2 Valve tubing to syringe hook up The valve s are de energized when the front mechanism 1 is running in the INFUSE direction The syringe...

Страница 13: ...zed Top Tube Bottom Tube Top Bottom Top Bottom Top Bottom Top Bottom Rear Front Fill Output Top Bottom Top Bottom Top Bottom Top Bottom Rear Front Fill Rear Output Front Fill Front Output Rear Figure 4 Pinch Valve Connections Figure 5 Continuous Delivery Pump in Reciprocal Mode Figure 6 Dual Delivery followed by Refill Pump in Parallel Mode ...

Страница 14: ...hould be finger tight plus 1 1 4 turns Tubing is 304 stainless 1 4 OD wall thickness 0 035 P Com B Normally Open A Normally Closed A Rear Front A P P B B Fill Output Figure 7 Continuous Delivery Pump in Reciprocal Mode A Rear Front A P P B B Output Front Output Rear Fill Front Fill Rear Figure 8 Dual Delivery followed by Refill Pump in Parallel Mode ...

Страница 15: ...he RS 232 connectors in the appropriate port according to the following chart Device Port Number Computer 1 Pump Chain 2 Configuring the Pump Chain After pressing SET and 1 the Remote LED will blink indicating the pump is request ing its pump chain configuration 1 First the display will show Adr nn with the colon blinking where nn is the current pump chain address Enter the 2 digit address assigne...

Страница 16: ...ted operation of the pump Timer Input Opening the timer input starts the pump Closing the timer input stops the pump The timer input allows for an externally controlled pumping interval Pumping Direction Input Sets the direction of pumping Opening the directional input sets syringe 1 to infuse Closing the input sets syringe 1 to refill The pumping direction input is not recognized when data is bei...

Страница 17: ...can still be controlled from the keypad while it is in a pump chain Control data that is changed via RS 232 will NOT be stored in the pump s non volatile memory unless requested with the SAV command or other data is changed from the keypad Each command sent to the pump chain is a string of ASCII characters with leading zero s on numbers and all spaces optional Numbers are a maximum of five digits ...

Страница 18: ...If pump already stopped a NotApplicable error is returned RAT A B float units Request to set or query infusion rate settings Set syringe 1 rate RAT rate RAT rate units RAT A rate RAT A rate units Set syringe 2 rate only valid in proportional mode RAT B rate RAT B rate units Rate is of the format f f f f f f Units are one of Meaning UM µl mn UH µl hr MM ml mn MH ml hr Rate 2 is only accepted when m...

Страница 19: ...Out Of Range error is returned Query syringe 1 diameter DIA DIA A Query syringe 2 diameter only valid in proportional mode DIA B Response is of the following format f f f f f f Units are millimeters MOD AUT PRO CON Request to set or query pumping mode SET Command Meaning MOD AUT Puts pump in Auto Stop mode MOD PRO Puts pump in Proportional mode MOD CON Puts pump in Continuous mode If pump is runni...

Страница 20: ...at the specified pin d on the external 9 pin D SUB connector Valid pin numbers for input are 6 7 and 8 Possible Responses Meaning ON TTL level high OFF TTL level low If the pin specified is invalid a Not Applicable error is returned OUT d ON OFF Request to set theTTL logic level at the specified pin d on the external 9 pin D SUB connector ON Set pin to a logic high OFF Set pin to a logic low Valid...

Страница 21: ... Commands Pump Chain Error Messages Error messages are in the format lf message cr and are followed by a prompt Message is one of the following Possible Responses Meaning Syntax error in a received command NA Command Not Applicable at this time OOR Out Of Range Control data is out of pump s operating range ...

Страница 22: ...0 1 46 250 2 30 500 3 26 1 0 ml 4 61 mm 2 5 7 28 5 10 30 10 14 57 Hamilton Microliter Series Gastight Size Diameter 0 5 µl 0 103 mm 1 0 1457 2 0 206 5 0 3257 10 0 460 25 0 729 50 1 031 100 1 46 250 2 3 500 3 26 1 0 ml 4 61 mm 2 5 7 28 5 10 3 10 14 57 25 23 0 50 32 6 Becton Dickinson Glass All Types Size Diameter 0 5 µl 4 64 mm 1 4 64 2 5 8 66 5 11 86 10 14 34 20 19 13 30 22 70 50 28 60 100 34 90 H...

Страница 23: ... used syringe manufacturers Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on manufacturer Syringe µl hr µl min ml hr ml min Size Min Max Min Max Min Max Min Max 10 µl 0 0073 950 05 25 µl 0 0183 2386 10 50 µl 0 0365 4772 50 100 µl 0 0731 9570 50 250 µl 0 1813 23 751 1000 µl 0 7281 95 418 1 ml 0 7828 102 580 2 ml 2 8493 373 430 2 5 ml 1 8156 237 950 3 ml 2...

Страница 24: ...ery Parallel Reciprocal IN d Read TTL pin d OUT d ON OFF Set TTL pin d SAV Save settings in NOVRAM VER Query software version units UM UH MM MH µl mn µl hr ml mn ml hr Command Response Output Format output response prompt response response response lf message cr prompt lf adr Stopped lf adr Syringe 1 infusing lf adr Syringe 1 withdrawing lf adr Pump stalled adr d d 1 or 2 digit pump address messag...

Страница 25: ...r Low Syring 1 Infusing High Syringe 1 Refilling 3 Pump Running Indicator Low Pumped Stopped High Pump Running 4 User Definable Output 5 User Definable Output 6 Foot Switch Input Falling edge starts stops pump 7 Timer Input Rising edge starts pump Falling edge stops pump 8 Directional control input Rising edge sets Syringe 1 to infuse Falling edge sets Syringe 1 to refill 9 Vcc Logic High Referenc...

Страница 26: ...Publication 5390 001 Revision B 25 Har vard Apparatus Model 33 Twin Syr inge Pump User s Manual Appendix F Pump to PC Connection 3 RJ11 RJ12 6 RJ12 6 x 6 22 2000 5 4 Front View 4400 Pump ...

Страница 27: ...1101 Solvents of any type should never be used to clean the pump A mild detergent solu tion may be used to clean the keypad Fuse Replacement Make sure power cord is disconnected from main supply before replacing fuse Remove bottom cover on pump by removing four 4 rubber feet and four 4 screws Locate fuse on power supply module Remove fuse from fuse clip Use caution no to break fuse when removing R...

Страница 28: ...flashing This indicates that the pusher block travel of the syringe corresponding to the syringe LED blinking has been impeded This may be caused by the syringe plunger hitting bottom a kink in the tubing syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and press RUN The ...

Страница 29: ...Cable for connecting two or more pumps MA1 55 4145 RS 232 Connector Cable for IBM and compatible personal computers MA1 55 4146 RS 232 Connector Cable for other than IBM and compatible personal computers The Harvard 33 Syringe Pump lends itself to a multitude of OEM industrial applica tions for all types of custom pumping or pilot plant applications Please contact the Harvard Development Group if ...

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