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If pH 4.01 buffer
solution is used
as the first point
the value of the
buffer is recognized
and displayed
with the blinking
stability indicator.
If pH 7.01 buffer solution is used as
the first point
the buffer is recognized
with the blinking stability indicator.
When the reading is stable, the
stability indicator will disappear
and pH 7.01 will be calibrated.
If pH 7.01 is the only calibration point,
finish one-point procedure at right.
If using pH 4.01 as a second point,
continue two-point procedure at right.
For one-point calibration using pH 4.01 buffer go to procedure B
For one or two-point calibration using pH 7.01 buffer go to procedure A
A
B
When the
reading is stable,
the stability
indicator will
disappear.“Sto”
will be displayed
when the
calibration
is saved.
Meter will
exit to
measurement
mode and the
calibration
tag will be
displayed.
Meter will exit
to measurement
mode and the
calibration
tag will be
displayed.
Tw
o-
Po
in
t
Use pH 4.01 to
perform a two-
point calibration.
The value is
automatically
recognized and
displayed with the
blinking stability
indicator.
When the reading
is stable, the
stability indicator
will disappear.
“Sto” will be
displayed when
the calibration
is saved.
Meter will exit
to measurement
mode and the
calibration
tags will be
displayed
.
Thank You
Thank you for choosing a Hanna Instruments product. Please read this instruction
manual carefully before using this instrument.
For technical support, contact your local Hanna Instruments Office or email us
at [email protected].
To find your local Hanna Instruments Office or for additional information on
Hanna Instruments products, visit www.hannainst.com
Meter Calibration
From measurement
mode, press and
hold the ON/OFF
button until “CAL”
is displayed.
When “7.01” blinks
on the display, place
the tip of the probe
into a pH 7.01 or
4.01 buffer solution.
On
e-
Po
in
t
One-Point Calibration with pH 4.01
B
A
One or Two-Point Calibration with pH 7.01
pH 4.01 will then
blink on the display.
Ignore it and press
ON/OFF button.
Operation
Press the ON/OFF button to turn the meter on. All tags
will be displayed.
The meter will go into measurement mode: current
reading and calibrated buffers are displayed.
Preliminary Examination
Remove the meter from the packing material and examine it carefully to make sure that
no damage has occurred during shipment. If noticeable damage is evident, contact your
local Hanna Instruments Office.
Each meter is supplied with:
• pH 4.01 buffer solution liquid sachet (2 pcs.)
• pH 7.01 buffer solution liquid sachet (2 pcs.)
• Cleaning solution for milk deposits ( 2 pcs.)
• Electrode storage solution, 13 mL dropper
• Instruction manual
• Quality certificate
Note: Save all packing material until you are sure that the instrument functions
correctly. All defective items must be returned in the original packaging with the
supplied accessories.
Meter Overview
Preparation:
The pH electrode is shipped with a protective cap containing storage solution. Before
using the meter,
remove the protective cap
and condition the electrode by soaking the
tip (bottom 4 cm (1.5”)) in pH 7.01 buffer solution for several minutes. Then follow the
calibration procedure.
• Do not be alarmed if white crystals appear around the cap. This is normal with pH
electrodes and they dissolve when rinsed with water.
• Turn the meter on by pressing ON/OFF button.
• Remove the protective cap and immerse the tip of the electrode in the sample
to be tested.
NEVER IMMERSE THE ELECTRODE OVER THE MAXIMUM IMMERSION LEVEL.
• Stir gently and wait for a stable reading.
• For best results, recalibrate periodically.
• After use, rinse the electrode with water and store it with a few drops of storage
solution in the protective cap.
• Reattach the protective cap after each use.
DO NOT USE DISTILLED OR DEIONIZED WATER FOR STORAGE PURPOSES.
Intended use
The measurement of pH in milk is important in testing for impurities, spoilage, and signs
of mastitis infection. While there are a number of factors that affect the composition of
milk, pH measurements can help producers understand what might be causing certain
compositional changes. pH measurement is commonly performed at various points in a
milk processing plant.
Milk with pH values higher than pH 6.7 potentially indicate that the milk may have come
from cows with a mastitis infection. Mastitis is an ever-present challenge with dairy milking
cows. When infected, the cow's immune system releases histamine and other compounds
in response to the infection. There is a resulting increase in permeability of endothelial and
epithelial cell layers, allowing blood components to pass through a paracellular pathway.
Sinceblood plasma is slightly alkaline, the resulting pH of milk will be higher than normal.
Typically milk producers can perform a somatic cell count to detect a mastitis infection, but a
pH measurement offers a quick way to screen for infection.
Understanding the pH of raw milk can also help producers optimize their processing
techniques. For example, in operations that use Ultra High Temperature (UHT) processing,
even small variations from pH 6.7 can affect the time required for pasteurization and the
stability of the milk after treatment.
Probe Features
Low Temperature Glass Tip and Open Junction.
The glass tip uses a special low temperature (LT) glass
formulation. This is beneficial since many food products are at
low temperatue. The open junction design consist of a solid gel
interface between the sample and the internal reference wire.
This makes it impermeable to clogging after measurements.
Conical Glass Tip
The conical shaped tip design allows for penetration into solids, semi-solids, and emulsions
for the direct measurement of pH in samples such as milk and yogurt.
Liquid Crystal
Display
ON/OFF
Button
Maximum
Immersion
Level
pH Probe
Calibration
tags
Battery
indicator
Unstable
tag
Measurement
unit
Digits
Recommended
Immersion
Level
Range
0.0 to 14.0 pH
Resolution
0.1 pH
Accuracy
±0.2 pH @25 °C/77 °F
Calibration
Automatic, one or two-point
Electrode
Built-in probe for specific application
Battery Type
CR2032 Li-ion
Battery Life
Approximately 1000 hours of continuous use
Auto-off
8 minutes, 60 minutes or can be disabled
Environment
0 to 50 °C (32 to 122 °F); RH 95% max
Dimensions
51 x 159 x 21 mm (2 x 6.3 x 0.9“)
Weight
50 g (1.8 oz.)
Specifications
"Sto" will be
displayed when
the calibration
is saved.