Grant-bio DEN-1B, Руководство по эксплуатации

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Table 1. Interpretati o n of McFarland Standard results into corresponding numeric values of
bacterial suspension concentration and the ir optical density at 550 n m.
* Bacterial concentration depends on microorganism size. The numbers represent an
average value valid for bacteria. For yeasts, which are larger in size, these numbers
should be divided by about 30.
** Values correspond to optical densities of bacterial suspensions. The BaSO solutions do
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not have the same optical density, because size and particle forms differ from those of
bacteria and light is diffracted differently.
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DEN-1B
Operating instructions
McFarland
Standard
0.5
1
2
3
4
5
Composition
Concentration
BaSO
4
-5
2.40 10 mol/l
-5
4.80 10 mol/l
-5
9.60 10 mol/l
-4
1.44 10 mol/l
-4
1.92 10 mol/l
-4
2.40 10 mol/l
Bacterial concentration*
6
150 x 10 cells/ml
6
300 x 10 cells/ml
6
600 x 10 cells/ml
6
900 x 10 cells/ml
6
1200 x 10 cells/ml
6
1500 x 10 cells/ml
Theoretical optical
density at 550 nm**
0.125
0.25
0.50
0.75
1.00
1.25
Interpretation
Version 1.01 - May 20 12