10
QUICK GUIDE
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PIPETMAN® L
Dispense
1.
Place the end of the tip against the inside wall of the
recipient vessel (at an angle of 10° to 40°).
2.
Press the push button slowly and smoothly to the first stop.
3.
Wait for at least a second, then press the push button to
the second stop to expel any residual liquid from the tip.
Keep the push button pressed fully and while removing the
pipette, draw the tip along the inside surface of the vessel.
4.
Release the push button smoothly. Eject the tip by pressing
firmly on the tip ejector button.
Eject the Tips
Before you start to pipette, you can adjust the tip ejector button
according to your preferences.
1.
Position the tip ejector button by rotating the tip ejector
button to the most comfortable position: left, right, or middle.
2.
Activate the tip ejector. You can either push the tip ejector
button with the tip of the thumb as usual, or with the base of
your thumb for more comfort.
Pre-Wet the Tips
Pre-wetting the tips before pipetting helps prepare the tips for
the best pipetting performance. Ideally, the pre-wet includes
both immersing the tip in the liquid and performing one pipetting
step. Pre-wetting the tips helps ensure that volumes that you
pipette will achieve accuracy and precision within specifications.
Aspirate
1.
Press the push button to the
first stop
(this corresponds
to the set volume of liquid).
2.
Hold the pipette vertically and immerse the tip in the
liquid (see immersion depth table below).
3.
Release the push button slowly and smoothly to top
position to aspirate the set volume of liquid.
4.
Wait one second (time depends on model, for more
details, please refer to PIPETMAN L User’s Guide
LT801575), and then withdraw the pipette tip from the
liquid. You may wipe any droplets away from the outside
of the tip using a medical wipe; however, if you do so,
take care to avoid touching the tip’s orifice.
Dispense
First stop
Second stop
Rest position
Prepare
Aspirate
Purge
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Figure 13
Pipetting motion - Aspirate and dispense
