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13
PIPETMAN L®
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USER’S GUIDE
Leak Test
This test may be performed at any time to check that the pipette does not leak, especially after performing
a maintenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal. After
making sure that the pipette is correctly reassembled, repeat this test.
For the P2L to P200L Single channel models, and the F1L to F250L Fixed models:
1.
Fit a PIPETMAN DIAMOND Tip.
2. For the
variable volume models
, set the pipette to the
maximum volume given in the specifications, and pre-rinse.
3. Aspirate the set volume from a beaker of distilled water.
4. Maintain the pipette in the vertical position and wait for 20
seconds.
5. If a water droplet appears at the end of the tip there is a leak.
6. If you see no droplets, re-immerse the tip below the surface of
water.
7. The water level inside the tip should remain constant; if the
level goes down there is a leak.
For the P1000L, P5000L and P10mL L Single channel models, for the F300L to F5000L Fixed models:
1.
Fit a PIPETMAN DIAMOND tip.
2. For the
variable volume models
, set the pipette to the maximum volume given in the specifications.
3. Aspirate the set volume from a beaker of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds.
5. If a water droplet appears at the end of the tip, there is a leak.
For the Multichannel models (8x - 12x):
1.
Fit the PIPETMAN DIAMOND tip.
2. Set the pipette to the maximum volume given in the specifications, and pre-rinse.
3. Aspirate the set volume from a reagent reservoir of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds ; fluid level in tips shoulds remain
constant.
5. If a water droplet appears at the end of the tip, there is a leak.
6. If you see no droplets, for volumes below 200 µL, re-immerse the tip below the surface of water.
7. The water level inside the tip should remain constant; if the level goes down there is a leak.
no leak
Figure 16
PIPETMAN L multichannel leak test.
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