Gilson F81040, Руководство пользователя

Страница: 5 / 21

P
iP
E
T
Ti
NG
M
O
d
E
S
P
iP
E
T
Ti
NG
M
O
d
E
S
9
8
PIPETMAN® M CONNECTED
|
USER’S GUIDE
PIPETTING MODES
|
PIPETMAN® M CONNECTED
4. Fit a tip suitable for the model
of PIPETMAN M Connected
that you are using (preferably
use PIPETMAN DIAMOND Tips
for assurance of accurate and
precise results; refer to Chapter
9 PiPETMAN diAMONd TiPS on
page 15).
5. Press the push button to aspirate
total volume. The volume
aspirated will be a little bit more
than required (extra volume). The
extra volume is necessary to ensure
equal operating conditions for each
dispensed aliquot.
dispense
Press the push button. diSCArd appears on the screen. Discard a part of the extra
volume and continue dispensing the aliquots. PIPETMAN M Connected distributes the
aliquot volume each time you click the push button. The number of aliquots left to
dispense is displayed on the screen. A warning beep indicates the last aliquot.
Purge
Press the push button. PUrGE appears on the screen. Click again to dispose and then
purge the rest of the extra volume and reset the piston. Keep your thumb pressing the
push button until the end of the purge—the piston reset takes place after you release
the push button again. This feature enables you to remove the tip from the liquid,
without aspirating anything .
Eject Tip
Press the ejection button.
MIX Mode
This a mixing phase, composed of repeatedly aspirating and dispensing.
1. Press the menu button to access menu, where you can choose between different
pipette modes. Select “MiX” Mode by turning the push button, and then to confirm
your selection, click on the push button.
Fit a tip suitable for the model of PIPETMAN M Connected that you are using
(preferably use PIPETMAN Tips for assurance of accurate and precise results, refer to
Chapter 9 PiPETMAN diAMONd TiPS on page 15).
1. Press the push button to aspirate the selected volume.
2. To dispense: click (press and release) the push button.
Three things will happen: sample dispense, automatic
purge, and piston reset to zero. Or, keep your thumb
pressing the push button until the end of the dispense
cycle and the piston reset takes place after releasing the
push button again. This feature enables you to remove
the tip from the liquid, without aspirating anything.
3. Eject the tip by pressing the ejection button.
Your PIPETMAN M Connected is ready for the next pipetting
cycle while maintaining the volume and the last settings.
Continue pipetting as you would with your mechanical PiPETMAN; it’s as simple as
that to use your new, electronic pipette!
REPETITIVE Mode
The rEPETiTiVE Mode allows you to distribute the same volume repeatedly in a
predefined number (N) of equal aliquots. You supply the aliquot volume
(AV) and the pipette automatically calculates the number of aliquots
possible from the nominal (maximum) volume (NV) of the pipette, as
follows:
N = NV/AV (e.g., 120 μL x 10 for P1200M).
You also can decrease the number of repetitions (e.g., 120 μL x 10-(n ≥ 1) for P1200M).
NOTICE
Always fit a tip
before using any
pipette.
Figure 3
REPETITIVE Mode
NOTE
The MiX Mode can be used to prepare a PCr master mix, enzyme restriction
mix, protein solution, oligonucleotide dilution and to mix samples with
gel loading buffer and so on… you can mix two different solutions or
homogenize one solution. if you work with higher viscosity than water (e.g.,
restriction enzyme), you may change the aspiration speed (refer to Chapter 8
PiPETTiNG SPEEd CONTrOL on page 15). if you work with genomic dNA, then
you should always pipette very carefully to avoid shearing and nicking.
NOTICE
Always fit a tip before using any pipette.
START button
Optional STOP
0
V
Purge
Figure 2
PIPET Mode
NOTE
The rEPETiTiVE Mode is ideal for dispensing aliquots. Examples include
dispensing a PCr master mix into PCr tubes or 96-well, pipetting elution buffer
for dNA extraction, preparing a target for spectrometric analyses, distributing
loading buffer into samples, etc.
1. Press the menu button to access the system menu, where you can choose between
different pipette modes. Select rEPETiTiVE Mode by turning the push button.
To confirm your selection, click on the push button.
2. Turn the push button half way, the screen will display:
“Click to change volume.”
3. Press the push button; the volume on the display will start to blink,
you can now adjust the volume.
Set the Aliquot Volume
1. Click the push button, set the volume by turning the push button and click again
to confirm your settings.
2. Set the aliquot number: the maximal number of aliquots has been calculated
by your PIPETMAN M Connected. The number of aliquots flashes and you can
decrease this number (N – (n ≥ 1)) by turning the push button.
3. Press again to confirm your settings.