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autoclart® plus User Manual

 

 

 

 

 

Version 3, February 2017

 

 

33 

 

 

Figure 14. Entry of samples. Positions sub-menu.

 

 

Automatic entry of sample references from files 

To enter references automatically from files, first contact the GENOMICA product specialist.  

 

17.3.

 

WORK LIST 

The work list will enable us to check all the data entered up to now is 

correct.  

If  you  should  need  to  correct  any data,  select  “Back”  (left  blue  arrow)  to  edit  the  test  or 

reference, as explained in the previous sections. 

If all the information is correct, press “continue” (right blue arrow) to start the analysis. 

 

 

Содержание Autoclart Plus

Страница 1: ...autoclart plus User Manual Version 3 February 2017 1 USER MANUAL autoclart plus User Manual...

Страница 2: ...ands registered by GENOMICA For greater detail in relation to the information set forth in this manual please access the following website www genomica com GENOMICA S A U Parque Empresarial Alvento Ed...

Страница 3: ...AME AND REFERENCES 9 7 DESCRIPTION 10 8 COMPONENTS CONSUMABLES 11 8 1 COMPONENTS 11 8 2 CONSUMABLES 12 9 ACCESSORIES 13 10 CHARACTERISTICS 15 11 UNPACKING STORAGE AND PACKING 16 11 1 UNPACKING 16 11 2...

Страница 4: ...17 1 ENTRY OF THE TYPE OF ASSAY 30 17 2 ENTRY OF THE SAMPLE REFERENCES 31 17 3 WORK LIST 33 17 4 ANALYSIS SETTINGS 34 18 ANALYSIS ADDING THE SAMPLE AUTOMATICALLY 34 19 ANALYSIS ADDING THE SAMPLE MANUA...

Страница 5: ...8 CARING FOR THE EQUIPMENT AFTER USE 64 29 FAQ 65 29 1 UPDATING AND INSTALLATION OF SOFTWARE 65 30 TECHNICAL ASSISTANCE SERVICE 66 APPENDIX A TABLE OF FREQUENT ERRORS AND RESPECTIVE SOLUTIONS 67 APPEN...

Страница 6: ...is is conducted using an image capturing and processing system and a specific algorithm for each kit The technique is based on a system designed to capture and process the images obtained in the micro...

Страница 7: ...rpretation of microarrays The analysis of the image is fully automatic preventing subjectivity and the interaction of the user thereby ensuring accurate results in a rapid and repeatable manner Compar...

Страница 8: ...pplied by GENOMICA Follow the instructions carefully We suggest you read these instructions before putting them aside They contain useful information on the correct and efficient management of the equ...

Страница 9: ...pursuant to Royal Decree 1662 2000 of 29 September 2000 and is classified under other products in Appendix III of the Directive Moreover GENOMICA S A U declares that the autoclart plus equipment has b...

Страница 10: ...capable of maintaining precise control over the temperature during the assay an incubation agitation system and a system to transfer the plate to the core of the equipment where the camera for captur...

Страница 11: ...e touch screen to view the number of samples and the assay to be executed whereby it is possible to process a minimum of 4 and a maximum of 96 samples per assay The equipment will conduct the analysis...

Страница 12: ...n the holder in the position marked 2 Connect the tube from the piston pumps to the top of the bottle 3 Tip rack holder Place the holder on the markers in the position marked 3 4 TL trough Place the T...

Страница 13: ...SSORY 1 SOFTWARE The analysis of the images and the interpretation of the results are conducted by specific software for each kit designed and implemented by GENOMICA GENOMICA distributes this softwar...

Страница 14: ...oclart plus in a manner other than that specified in this manual will result in the loss of the guarantee provided Do NOT use any accessory which has not been supplied by GENOMICA GENOMICA will not be...

Страница 15: ...ntrol PID control Processing volume 3 L to 100 L Dispensing and tolerance system Piston pumps 3 10 L 10 100 L 0 5 Software Integrated software Network voltage and frequency 100 to 240 VAC with 5A grou...

Страница 16: ...may result in serious injury to the user or damage to the equipment In order to ensure the protection this equipment provides is not damaged do not use or install it in a manner other than that speci...

Страница 17: ...the equipment out of the container one in front of it and one behind it 7 Inspect the equipment to check for any clear signs of damage during transportation Report any damage to your local representa...

Страница 18: ...ponents which operate in a vertical position and care should be taken not to twist the structure of the equipment while it is being stored This manual should be kept close at hand for everyday referen...

Страница 19: ...in place 12 ENVIRONMENTAL CONDITIONS 12 1 VENTILATION Take the following recommendations into account Do not place the equipment in areas in which there are currents of air Moreover the equipment does...

Страница 20: ...led wait for 1 hour before turning it on to avoid potential problems caused by condensation All waste liquid is stored in the equipment until the end of the test and as such it does not need to be dra...

Страница 21: ...se acknowledge and comply with all the warning signs on the equipment and its accessories CAUTION Do not operate instrument with cover open Risk of injury CAUTION Risk of trapping fingers during instr...

Страница 22: ...UV decontamination procedure Take special care with the electrical components Comply with safety measures at all times Repairs to the electrical system may only be conducted by qualified personnel Do...

Страница 23: ...uipment on a firm flat surface The assembly surface should be level in all directions to ensure the equipment functions correctly Make sure autoclart plus is installed on a totally horizontal stable s...

Страница 24: ...0 230 V AC and 50 60 Hz and that the system is earthed Do not install autoclart plus near a source of light intense heat or external vibrations Never touch the switches with wet hands Turn the equipme...

Страница 25: ...he other side to limit the inclination of the touch screen We recommend requesting the assistance of a second person from this point on Figure 4 The yellow circle highlights the M5 screws for the brac...

Страница 26: ...ion to earth Press the ON button Figure 5 Power cable and ON switch Once it has been turned on autoclart plus will take a few seconds to start up During this time the device will conduct a series of i...

Страница 27: ...the waste in accordance with local and state provisions 4 Remove the reagents troughs and dispose of the waste in accordance with local and state provisions 5 Clean the reagent troughs with water see...

Страница 28: ...ART OF THE ANALYSIS The reading and analysis of a sample is a simple sequential process consisting of a series of menus from which to select the different options involved in the process Press the new...

Страница 29: ...ry 2017 29 Figure 9 Diagram of a microplate on the New Analysis menu This is the application s most important menu as it enables the user to establish The number of samples to be analysed The assay re...

Страница 30: ...test provided by GENOMICA and the CS in which the assay has been defined will automatically change to its specific colour Once the process has been concluded press the tick Figure 9 Selection of auto...

Страница 31: ...es N B Remember that if you intend to conduct the same assay in all the bands press AUTO SEL after selecting the assay The symbol A will appear alongside the assay selected 17 2 ENTRY OF THE SAMPLE RE...

Страница 32: ...s added which will always occupy consecutive positions Once all the references have been added press the tick to return to the previous menu Figure 13 Entry of samples Manual entry of sample reference...

Страница 33: ...lly from files first contact the GENOMICA product specialist 17 3 WORK LIST The work list will enable us to check all the data entered up to now is correct If you should need to correct any data selec...

Страница 34: ...dd samples automatically To add samples manually For reading only Select the desired option on the touch screen Figure 15 Analysis settings 18 ANALYSIS ADDING THE SAMPLE AUTOMATICALLY Select the add s...

Страница 35: ...on 1 ml tip racks at the back of the holder red rack 10 l tip racks at the front of the holder white racks Ensure the racks are full Remember that the consumption of 1 ml tips is the same for all assa...

Страница 36: ...insert the plate with as many CS as required for the analysis of the samples as illustrated in the image on the right Ensure position A1 is in the top left corner Figure 19 Position of the microplate...

Страница 37: ...37 Step 3 11 CHECK THE USED TIP TROUGH IS IN PLACE AND EMPTY Figure 21 Empty tip trough Step 4 11 CHECK THE WASTE TROUGH IS IN PLACE AND EMPTY Figure 22 Empty waste trough Step 5 11 CONNECT THE TUBE T...

Страница 38: ...on Attach the connecting tube to the waste trough and put on the cover as illustrated in the image on the right Figure 23 b Connecting the tube to the waste trough as highlighted in yellow Step 6 11 L...

Страница 39: ...tilled water Tube inserted through the red cap REMEMBER THAT REAGENTS AND THE AMPLIFICATION PLATE SHOULD NOT BE ADDED UNTIL THE TROUGHS HAVE COOLED DOWN Figure 25 autoclart plus audio signal highlight...

Страница 40: ...40 Take note that the viewing reagent troughs only fit in the correct position TL SH Troughs CJ RE Troughs Figure 26 Position of the viewing reagent troughs Step 7 11 PLACE THE SPECIFIED VOLUME OF WA...

Страница 41: ...D VOLUME OF HYBRIDISATION SOLUTION IN THE TANK Figure 28 Volume of hybridisation solution Step 9 11 PLACE THE SPECIFIED VOLUME OF DILUTED CONJUGATE IN THE TANK Figure 29 Volume of conjugate Step 10 11...

Страница 42: ...place the PCR plate in the holder Check the PCR plate is in the correct position and position A1 is in the top left corner and press it firmly downwards Figure 30 b Loading the PCR plate Position in t...

Страница 43: ...tinue button white arrow to start the analysis The screen will show the estimated time of the end of the assay the processing phase which will change throughout the assay and the warning not to open t...

Страница 44: ...n completed close the cover to the equipment and press the continue button white arrow to start the analysis When the equipment reaches the sample addition step it will emit an audio signal and the me...

Страница 45: ...the microplate with the desired number of CS to be read and turn the lock Close the equipment and press the white arrow to continue 21 READING When the test has been concluded the equipment will autom...

Страница 46: ...f the reading When the progress bar reaches 100 the test has been completed and the results can be viewed exported and or printed out 22 RESULTS 22 1 VIEWING RESULTS Press report to view the results o...

Страница 47: ...the specific report on the sample Figure 37 Individual results The following information is available on this sub menu Raw data Provides access to the numeric raw and standarised values obtained from...

Страница 48: ...ill appear of all the assay samples from which to select the results you wish to export Select the samples you wish to export This can be conducted individually or by selecting the entire assay by pre...

Страница 49: ...r each of the samples see item 22 1 The work list includes the following information Date and time of the process Position of the sample in the microplate The code of the analysis conducted The type o...

Страница 50: ...ll be available for reanalysis Figure 42 Initial menu of the reanalysis option Change of assay reference First of all check the assay selected is the correct one and if not change it To do so press th...

Страница 51: ...in place simply press on them on the screen and drag them into their final position Once the markers are in position the software provides a general view of how the grid would fit on the array If the...

Страница 52: ...the grid manually implies the intervention of the user in the results which will be registered in the final report generated in the form of the following message The image has been reanalysed by the...

Страница 53: ...ORDS The records option on the main menu provides a results search tool Figure 46 The main menu Access to assay records This results search tool can be used for an analysis or run or a sample 22 1 SEA...

Страница 54: ...meter There are three different search parameters to choose from run ID assay and plate Figure 48 Search parameters Once the parameters have been established a list will appear with all the assays whi...

Страница 55: ...he Search by run procedure which will enable the user to set the parameters by date run ID assay ID plate ID and particularly sample ID Once the parameters have been established a list will appear wit...

Страница 56: ...ATING A PROGRAM Select the install update option to access a sub menu containing a list of all the programs currently installed in the equipment and their version Selecting a specific program and pres...

Страница 57: ...ENOMICA to install the program The installation is automatic Figure 53 Assay install update menu 22 4 SYSTEM The System option provides the user with information on the software hardware and the seria...

Страница 58: ...ent s memory The final destination should be specified prior to exporting or backing up data The information contained in autoclart plus may be stored on any external memory device with a USB connecti...

Страница 59: ...ols for the maintenance of the equipment Decontamination This program decontaminates the equipment using UV light Cleaning This program cleans the equipment s tubing and pump system Technical Assistan...

Страница 60: ...ridisation solution trough 6 Ensure neither the PCR plate nor the CS microplate has been loaded 7 Close the equipment door Once completed press continue Up to this point the user may reverse the proce...

Страница 61: ...ectant whenever no foam is formed Avoid all soapy solutions Check the waste trough is present and empty Once completed press continue Up to this point the user may reverse the process by pressing the...

Страница 62: ...e box back in place by pressing down firmly on the middle of the fuses 3 The type of fuse is indicated on the autoclart plus label T5A 250V 5 X 20 MM 27 CLEANING The equipment should be cleaned on a r...

Страница 63: ...lean all the reagent trays at the end of each run and ensure the waste trough has been emptied We recommend the use of UV goggles as a precautionary measure when conducting the UV decontamination The...

Страница 64: ...recycling please contact GENOMICA or the supplier of the equipment for instructions on how to return equipment whose life cycle has ended for appropriate disposal Packaging Please keep the packaging m...

Страница 65: ...browser which is normally identified with the letter D 5 autoclart plus may take a few minutes to recognise the memory Return to the main menu wait for a moment and try again 6 Access the USB memory...

Страница 66: ...on from step 4 When you have finished return to the main menu and reboot the equipment In the event error messages appear please contact your technical assistance department Remember to quote these me...

Страница 67: ...re the tip picking removal system is in the initial position move the X and Y axes by hand to check for obstructions In the event of obstructions look for mechanical impediments such as loose screws C...

Страница 68: ...or code Contact the technical assistance department 3 Start up of module AZ Home sensor not detected Error code Contact the technical assistance department 4 Start up of module Z Home sensor not detec...

Страница 69: ...will stop immediately and an error code will appear on the touch screen The error codes are stored in a register ALL CASES of error codes S should be reported to the service provider immediately for...

Страница 70: ...s ACP Evaluation procedure Appendix III to Directive 98 79 CE Declaration of CE Conformity Classification Class A Rule 5 Appendix VII Genomica S A U hereby declares that the product autoclart Plus com...

Страница 71: ...rsion 3 February 2017 71 APPENDIX C ABBREVIATIONS USED CS CLART strip Fig Figure PCR Polymerase Chain Reaction PDF Portable Document Format generated by Adobe LIMS Laboratory Information Management Sy...

Страница 72: ...the event the normal robot function is interrupted This symbol warns of the danger of possible damage or harm caused by the high voltage of the equipment This symbol refers to important information on...

Страница 73: ...nate Current A C Identifying label on the equipment Symbol and Border White IEC 60417 5007 ON On switch with filter Symbol and Border White IEC 60417 5008 OFF On switch with filter Rear Yellow Symbol...

Страница 74: ...3 February 2017 74 APPENDIX E DECONTAMINATION CERTIFICATE Decontamination Certificate This equipment Model ____________ Serial number ____________ Has not been in contact with biological samples of a...

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