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29-0047-78 AC 04-2014

8

 

•   The reference solution is then removed, the cuvette filled with the sample and the sample measured by pressing the run 

button on the keypad. The measured results will be displayed on the instrument display.

 

•   The Ultrospec 7000/8000/9000 instruments have a colored Touchscreen for operation and a double beam optical 

system. In normal operation a cuvette filled with reference solution is put in the rear cell holder , a cuvette filled with 
sample in the front cell holder, the relevant application method is loaded and the Run Icon pressed. If a single cell is being 
used e.g. a microcell it should be filled with reference solution, placed in the front cell holder, the Zero icon pressed, the 
cuvette filled with sample, placed in the front cell holder and the Run Icon pressed

•  Typical results screen (DNA application GeneQuant)

2.3. Performing a run - micro volume instruments

•   On the SimpliNano units samples are measured in a sample port. Using a pipette 

a solution of reference material, which is typically the solution that the sample is 
dissolved in, is dropped onto the measurement position in the sample port and the 
instrument zeroed by pressing the Reference key on the keypad 

 .

Zero

Run

            Ultrospec 10                            Novaspec III/Plus                       GeneQuant 100/1300                      Ultrospec 2100                    

Содержание SimpliNano

Страница 1: ...Spectrophotometer Health Safety Document including General Operating Instructions Original Instructions ...

Страница 2: ... 2 Performing a run cuvettes 7 2 3 Performing a run Micro Volume Instruments 8 2 4 Post run procedures 9 2 5 User Maintenance 9 2 6 Novaspec III Plus Lamp Change 10 2 7 Ultrospec 8000 9000 Lamp Change 10 3 Troubleshooting 11 3 1 Emergency Procedures 12 3 2 Recycling Procedures 12 3 3 Recycling of hazardous substances 12 3 4 Disposal of electrical components 12 3 5 Specifications 13 3 6 Manufacturi...

Страница 3: ...onto the measurement area Typical applications of spectrophotometers include DNA RNA Oligo concentration and purity measurements as well as protein concentrations This manual addresses the needs of scientists and technicians who operate UV Visible spectrophotometers The level of information presented in this manual assumes the user possesses basic laboratory and technical skills and has the knowle...

Страница 4: ...clude an appropriate decontamination certificate 1 4 General Safety This equipment has been designed to conform to the following directives 2006 95 EC Low voltage directive LVD 2004 108 EC Electromagnetic Compatibility EMC directive 2012 19 EU Waste Electrical and Electronic Equipment directive recast WEEE Recast 2011 65 EU Restriction on the use of certain hazardous substances ROHS directive 2006...

Страница 5: ...y be connected to a separate PC To preserve the integrity of the measuring equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the information that came with your PC In common with all computer operation the following safety precautions are advised To reduce the chance o...

Страница 6: ...ng capacity SimpliNano units 100 240 V AC 50 60 Hz 3 A UK plug only NanoVue Plus units 90 250v 50 60Hz Max 30 VA 3 A UK plug only The instrument should be positioned so that the power cable may be readily removed in the event of a hazard or malfunction occurring Site the instrument in an atmosphere free from dust and corrosive fumes Ultrospec 2100 instruments have the on off switch on the back of ...

Страница 7: ...ntration purity The options within the application set up the instrument parameters such as wavelength and define any calculations taking place on the measured results 2 2 Performing a run cuvettes On the Ultrospec 10 Novaspec III Plus GeneQuant 100 1300 and Ultrospec 2100 7000 8000 9000 units samples are measured in a cuvette A suitable cuvette must be used for the application to be carried out i...

Страница 8: ... and the Run Icon pressed If a single cell is being used e g a microcell it should be filled with reference solution placed in the front cell holder the Zero icon pressed the cuvette filled with sample placed in the front cell holder and the Run Icon pressed Typical results screen DNA application GeneQuant 2 3 Performing a run micro volume instruments On the SimpliNano units samples are measured i...

Страница 9: ...ons or dilute detergent solutions but should always be rinsed in deionised water SimpliNano wipe the sample port with a dry lint free tissue between samples and at the end of the run this is sufficient to remove sample If more thorough cleaning is desired the port can also be cleaned with dilute 2 detergent solution followed by isopropyl alcohol NanoVue Plus wipe sample with tissue At end of sampl...

Страница 10: ...ose of the faulty lamp in accordance with local regulatory practice 2 7 Ultrospec 8000 9000 Lamp Change 1 Switch the instrument off disconnect the power supply cord and allow lamps to cool 2 Locate the lamp access cover at the rear of unit and remove the two top screws and slide top cover back and lift off Never operate the unit with the lamp housing cover removed 3 4 Remove the lamp and dispose o...

Страница 11: ...ot zero Cuvette placed in wrong orientation Wrong cuvette material used for wavelength Novaspec III Error code 001 symbol Lamp needs changing Novaspec III Error code 006 symbol Pixel clock too high Instrument needs optical alignment Contact service support Novaspec III Error code Flashing number Wavelength accuracy failure Instrument needs optical alignment Contact service support Novaspec plus X ...

Страница 12: ...ual supplied on a CD with the instrument 3 1 Emergency Procedures In the event of a malfunction or hazard occurring the user responsible shall disconnect the unit from power and isolate the instrument for decontamination by an appropriate means if bio hazardous material is spilt on in or around the instrument Ultrospec 2100 instruments have the on off switch on the back of the instrument on the ri...

Страница 13: ... 0 004 A at 1 A 0 006 A at 2 A at 440 nm 465 nm 546 1 nm 590 nm 635 nm 0 005 A using 60 mg l K2Cr2O7 within 0 5 of Absorbance value to 3 000 A at 546 nm 0 005 Abs or 1 of the reading whichever is the greater at 546 nm 2 0 or 0 010 A to 1 000A at 546nm whichever is the greater 0 05 A at 1 A using Neutral Density Filters Full specification and operating instructions are contained within the relevant...

Страница 14: ...to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information http www gelifesciences com GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences AB Björkgaton 30 751 84 Upps...

Страница 15: ...29 0047 78 AC 04 2014 15 Page intentionally left blank ...

Страница 16: ...l Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan For contact information for your local office please visit www gelifesciences com contact GE Healthcare Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK http www gelifesciences com imagination at work 29 0047 78 AC 04 2014 imagination a...

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