GE SEMIPHOR TRANSPHOR Series, Руководство по эксплуатации

Страница: 44 / 69

Assemble a transfer stack with
multiple gels
Follow the instructions below to assemble a vertical transfer stack.
When transferring multiple gels, transfer efficiency depends on factors such
as gel thickness, gel position in the stack, transfer buffer, membrane type, and,
most importantly, the characteristics of the protein. The gel closest to the anode
generally transfers better than the gel that is further away fro the anode. It is
preferable to lay gels side-by-side rather than stacking them.
Tip:
Action Step
Follow the instructions in Preparations, on page 38 . 1
Follow the instructions in Assemble a transfer stack with a single gel, on
page 39 until step 3.
2
NOTICE
Stack each layer with care, with edges parallel. As each
layer is added, remove all air pockets by rolling a clean
pipet from the center to the edges. Add a few drops of
buffer to trouble areas to help remove air pockets.
Note:
For best results, the transfer stack should be centered on the electrode panels.
Continue the stack as shown in Illustration of a transfer stack with multiple
gels, on page 40 .
3
Cut a porous cellophane sheet to size that is slightly smaller than the gel.
Note:
Cellophane permits electric current to pass but stops proteins.
4
Wet the cellophane sheet with transfer buffer. 5
Place the cellophane sheet on the gel. 6
Repeat steps 1 to 3 in Assemble a transfer stack with a single gel, on page 39 . 7
Place several sheets of buffer-soaked blotting paper on the gel. 8
Place the cover on top of the transfer stack.
Note:
Do not remove the cover until after the transfer is complete in order to prevent
stack components from moving.
9
44 SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
5 Operation
5.1 Assemble transfer stack