GE RPN2512E Скачать руководство пользователя страница 16 | Manualshive

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GE RPN2512E Скачать руководство пользователя страница 16

Appendix 3. Radioactive labeling

systems

Labelling

Technology

Nucleotide

Amount of

Labelling

Probe specific

Recommended

Product

system

template

time

activity

application

code

(dpm/µg)

Rediprime™ II

Random-prime

dCTP only

25 ng

10 minutes

2 × 10

9

membrane

RPN1633

hybridization

RPN1634

Ready-To-Go™

Random-prime

dCTP only

10 ng–1 µg

5 minutes

2 × 10

9

membrane

27-9240-01

DNA labelling

hybridization

beads

DNA labelling

Megaprime™

Random-prime

any dNTP

25 ng

10 minutes

2 × 10

9

membrane

RPN1604

hybridization

RPN1605

RPN1606

RPN1607

Nick translation

Nick translation

any dNTP

1 µg

2–3 hours

2 × 10

9

production of

N5000

large amounts

N5500

of probe

RPN2510EUM Appendix 3 Rev C 06/2007

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Содержание RPN2512E

Страница 1: ...GE Healthcare Amersham Hybridization oven shaker Product User Manual Codes RPN2510E RPN2511E RPN2512E...

Страница 2: ...branes into bottles 10 7 2 Hybridization 10 7 3 Membrane washing procedures 11 7 3 1 Radioactive Hybridizations 11 7 3 2 AlkPhos Direct Hybridizations 11 8 Maintenance care cleaning of the oven shaker...

Страница 3: ...perty of their respective owners 2003 2007 General Electric Company All rights reserved First published November 2003 All goods and services are sold subject to the terms and conditions of sale of the...

Страница 4: ...eed platform shaker for box hybridizations depurination denaturation and neutralization steps The instrument is Economical bottle hybridization minimizes probe volumes reducing reagent volumes and enh...

Страница 5: ...been supplied with a cordset and plug Check that the appropriate plug for your electrical supply has been included If there is any doubt consult a qualified electrician and inform your local GE Health...

Страница 6: ...rical Nominal Voltage Hertz Amp Product code 230 V 50 Hz 3 1 A RPN2510E 110 120 V 60 Hz 4 A RPN2511E 100 V 50 60 Hz 4 A RPN2512E Each instrument is supplied with 1 rotisserie RPN2514 6 hybridization b...

Страница 7: ...n Shaker is found on the product information label on the rear of the instrument 5 Turn the Mains ON OFF switch 1 on Fig 1 to the ON position 6 The GE Healthcare Hybridization Oven Shaker is now ready...

Страница 8: ...o use the platform shaker for sandwich box hybridizations the following procedure should be adopted 1 Open the oven door to its fullest extent lift the rotisserie vertically and store in a safe place...

Страница 9: ...sufficient prehybridization buffer to ensure that the entire surface of the membrane is covered Recommended volume surface area ratio is given in most protocol booklets Seal the box or bag securely 4...

Страница 10: ...se membranes These handling advantages should be considered against the potential loss of sensitivity before use When a hybridization mesh is used in conjunction with the membrane the following proced...

Страница 11: ...e bottle and the membrane thoroughly with 2 SSC and discard 2 Perform the following stringency washes in large volumes 100 ml minimum of the following solutions at a rotisserie speed of 8 rpm 2 10 min...

Страница 12: ...be used Fitting of non approved parts may affect the performance of the safety features designed into the instrument 8 1 Error codes faults The Oven has built in fault diagnostics If a fault occurs t...

Страница 13: ...ecommended hybridization buffer or extend prehybridization time 2 4 Increase number of buffer changes during the washing stage or increase stringency of final wash 3 1 Load extra lanes with control DN...

Страница 14: ...078 100 1200 1500 872 50 1100 1000 603 1000 500 564d 900 310 800 281 700 271 600 234 500 194 400 125c 300 118 200 72 100 a not necessarily the largest size fragment possible only the largest that is r...

Страница 15: ...RPN910D 10 10 cm 10 sheets RPN1010D 15 20 cm 10 sheets RPN1520B RPN1520S RPN1520N RPN1520T RPN1520D 20 20 cm 10 sheets RPN2020B RPN2020S RPN2020N RPN2002T RPN2020D RPN2020E RPN2020F 22 2 22 2 cm 10 sh...

Страница 16: ...109 membrane RPN1633 hybridization RPN1634 Ready To Go Random prime dCTP only 10 ng 1 g 5 minutes 2 109 membrane 27 9240 01 DNA labelling hybridization beads DNA labelling Megaprime Random prime any...

Страница 17: ...before Quantification Recommended application system hybridization light output re probing to detection AlkPhos Direct RPN3690 0 06 pg 1 hour 5 days yes no Single copy Southern and Northerns ECL Direc...

Страница 18: ...MP 35 43 cm 50 28 9068 48 Hyperfilm MP Enveloped 18 24 cm 50 28 9068 50 Hypercassette cassettes for autoradiography and light detection Size Code standard Code deep 18 24 cm RPN11642 RPN11628 24 30 c...

Страница 19: ...bridization buffer Product name Pack size Code Rapid Hyb buffer Rate enhanced hybridization buffer 125 ml RPN1635 for use with radiolabelled nucleic acid probes 500 m RPN1636 RPN2510EUM Appendix 6 Rev...

Страница 20: ...ray RPN2083 455 255 mm 1 tray RPN2093 Safety Tray Liners to match tray 685 455 mm 25 liners RPN1528 to match tray 685 530 mm 25 liners RPN2048 to match tray 530 330 mm 25 liners RPN2058 to match tray...

Страница 21: ...21...

Страница 22: ...ealthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan GE Healthcare...

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