
Scanning mode
Description
Scan setting
D
3
P
2
F
1
X
X
X
Selects a pre-programmed method for
scanning.
Note:
In the Fluorescence mode, up to five sequen-
tial scans can be performed in one method.
X
4
X
X
Selects the pixel size for scanning.
A sample with a smaller pixel size can be
analyzed in more detail, but the scanning
time and the image file size will increase.
X
X
Selects the voltage of the photo-multiplier
tube (PMT).
The higher the PMT voltage, the higher the
sensitivity. However, a too high PMT voltage
may lead to image saturation and a high
background. It is therefore recommended
to optimize PMT voltage for each sample
type.
X
Sets the voltage of the PMT automatically
based on a pre-scan.
X
Selects the scan speed: Normal or Slow.
Normal scan speed is recommended for
most applications.
To reduce background noise, use a slow
scan speed. A slow scan speed reduces noise
by data averaging.
X
Adds additional scans.
X
Changes between scan areas (DIGE method).
X
Adds an annotation (DIGE method).
An annotation can be deleted with the small
cross.
1
Fluorescence mode
2
Phosphor Imaging mode
3
Densitometry mode
Amersham Typhoon Operating Instructions 29193226 AC
61
4 Amersham Typhoon Control Software
4.1 Main window