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5 Methods
5.6 Cycle types
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Biacore T100 Software Handbook BR-1006-48 Edition AE
The High performance and Low sample consumption options support a Mix
function for mixing sample with a defined solution in the autosampler before
injection. Check the
Mix option and enter a mix solution and mixing fraction to
use this function. Entering a fraction of e.g. 20% will mix one part of mixing
solution with four parts of sample. The sample and mixing solution are taken
from respective positions in the autosampler and mixed in a third position. The
option
Stabilization period after mix allows you to specify a wait period
between the mixing operation and injection of the mixed solution. Mix is not
supported for the Single cycle kinetics option.
Solvent correction command
This command injects a 30-second pulse of solvent correction solution over the
surface at a flow rate of 30 µl/min. A solvent correction cycle should contain 4-8
Solvent correction commands for the different solvent concentrations used to
construct the correction curve (see Section 6.7). Solvent correction commands
will be correctly evaluated only when they are used in cycle types that are
connected to assay steps with purpose
Solvent correction .
5.6.2 Variables
Parameters for many of the commands in a cycle definition may be set as
variables. Values for variables are entered in either the
Variable Settings in the
method or the Setup Run step when the method is run (Sections 5.7 and 5.9.2),
and determine the number of cycles that will be performed in the run. Variables
fall into two broad classes:
• Method variables such as sample name or contact time control the way
the cycle will be performed. Parameter values that are not set as variables
are defined in the main command panel.
• Evaluation variables such as concentration or analyte molecular weight
are used in evaluation of the data. Some evaluation variables are required
for correct functioning of application-specific evaluation procedures (for
example, kinetic evaluation requires a variable called
Conc which holds
the analyte concentration). These are selected from a predefined list.
Other evaluation variables may be freely defined by the user, to hold
Single cycle kinetics Injects a series of sample concentrations in the same
cycle, intended for single-cycle kinetics analysis (see
Section B.9). The samples are injected in direct
sequence, separated only by the time required to
prepare the next injection. A dissociation time is
included after the last sample injection. With this option
checked, an evaluation variable (see Section 5.6.2) will
be set up for each of the specified number of
concentrations.