FujiFilm FLA-8000 Скачать руководство пользователя страница 94

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2002. Jan. Verson 3.0

.... The red frame of the small image corresponds to the part

displayed in the image window.

..................... To enlarge a displayed image, click the

button, and click

the mouse button in a position to be enlarged in the image window.

.................... To contract a displayed image, click the

button, and click

the mouse button in a position to be contracted in the image window.

.................... To move a displayed image without changing the magnification,

click the

button and drag the mouse in the image window.

5-2. Enlarging or contracting the image display size

10-3. Changing the color of displayed images

You may change the color of displayed images by clicking one of six buttons
shown above.

Scanning Gel Samples (Scan Mode)

Содержание FLA-8000

Страница 1: ...FLA 8000 OPERATION MANUAL 2002 Jan Version 3 0 Version 3...

Страница 2: ......

Страница 3: ...rd Prevention Related to Radioisotope Pollution 10 Controlled area 10 Limit of superficial pollution 10 Installation site of instrument 10 Carrying out of the controlled area 10 7 Radioisotope RI Poll...

Страница 4: ...s 54 Part 6 Scanning IPs 57 Part 7 Scanning Gel Samples 77 Part 8 Setting Optional Filters 101 Part 9 Daily Maintenance 105 1 Maintenance of SHG Laser 106 Part 10 Troubleshooting 107 Troubles in FLA 8...

Страница 5: ...2002 Jan Verson 3 0 3 Part 1 PRECAUTIONS...

Страница 6: ...rocution Do not place a heavy object on this instrument If it falls down or drops it may cause injury WARNING CAUTION Do not modify this instrument Unauthorized modification may cause a fire electric...

Страница 7: ...4 Preventing lightning damages This instrument requires class D grounding Class D grounding prevents electric shocks or electrocution in the case where electric current leaks from an instrument whose...

Страница 8: ...sed Applicable safety standards This instrument conforms to the above mentioned standards and is designed to be safe against laser radiation The laser leak level is suppressed below 22 1 mJ for suffic...

Страница 9: ...2002 Jan Verson 3 0 7...

Страница 10: ...detach the cover you may pos sibly get an electric shock or be electrocuted in the worst case This instrument fits or conforms to the regulations and standards shown below Standard List EMC Japan VCCI...

Страница 11: ...ple condition and it is quite difficult to foresee the degree of pollution Radioisotope pollution may harm the FLA 8000 body due to improper operation or an unexpected trouble The performances of the...

Страница 12: ...s Non RI method Therefore it is recommended that the user should install it outside the controlled area and use RI indicated samples without contact ing them with IPs directly When a sample is in dire...

Страница 13: ...pollution may be tran scribed to the non polluted IPs Be very careful IPs may be used repetitively and accordingly should be protected against RI pollu tion If an RI sample is used in direct contact w...

Страница 14: ...irect sunlight which is not suitable to IP reading and may cause temperature rise inside the instrument or shield leak age resulting in a failure of proper operation of the instrument Install the inst...

Страница 15: ...ION The slide carrier weighs 420 g If it drops onto your feet your feet will be injured The slide carrier consists of precision parts Handle it with great care If it drops or impact or external force...

Страница 16: ...drop it Never put any objects other than samples on the glass or apply pressure to the glass Be very careful not to break the gel carrier glass Broken glass may cause injury The gel carrier with the...

Страница 17: ...3 0 15 IP Carrier The IP carrier weighs 370 g If it drops onto your feet your feet will be injured The IP carrier becomes unusable if it drops and deforms or its ends are lost IP Carrier 11 Other Inst...

Страница 18: ...2002 Jan Verson 3 0 16...

Страница 19: ...2002 Jan Verson 3 0 17 Part 2 SYSTEM CONFIGURATION...

Страница 20: ...ture saves costs Besides compactly designed IPs are easier to handle than the traditional X ray film With these features a single FLA 8000 enables not only gel imaging of DNA RNA and protein but also...

Страница 21: ...to the analyzer unit Hereinafter the instrument body is merely referred to as the body in this manual 2 Standard System Configuration 2 AC Power Cable The AC power cable is exclusive for this instrum...

Страница 22: ...ded in the accessories mount the IP filter and set the folder to the instrument 7 Reading Software Image Reader Install Image Reader in the analyzer unit and set the reading conditions to control the...

Страница 23: ...2002 Jan Verson 3 0 21 Part 3 Switching On and Off...

Страница 24: ...ions as shown below Operation Procedures Switching On and Off the FLA 8000 Switching On Switching Off Operation Procedures Immediately after the FLA 8000 is switched on The POWER lamp goes on During s...

Страница 25: ...2002 Jan Verson 3 0 23 Part 4 Scanning Slide Glass Samples...

Страница 26: ...on the PC 3 Make sure that warming up is completed and the FLA 8000 is ready Activate the FLA 8000 Image Reader from the Startup menu or using the shortcut key 4 The main window of the FLA 8000 Image...

Страница 27: ...side fully to the very end through the opening of the slide glass carrier Note In an image scanned by the FLA 8000 the slide numbers on the slide glass carrier are displayed in the upper part of the m...

Страница 28: ...2002 Jan Verson 3 0 26 2 3 Open the sample setting door 2 4 Open the carrier locking cover Scanning Slide Glass Samples Carrier Set Mode...

Страница 29: ...oles in the carrier 2 6 Close the carrier locking cover firmly Close the carrier locking cover so that the poles on both sides of the carrier locking cover are securely engaged with the pole holds on...

Страница 30: ...oor 3 Go back to the PC and click on Setting in the PC window On the right of Carrier Set in the upper part of the window or lower left of the window 4 The window for setting the scanning conditions a...

Страница 31: ...scanning mode speed Std Fast Scans pixels at 400 mm second High Sens Slow Scans pixels at 200 mm second The sensitivity is higher at the slower scanning speed 1 3 Slide glass selection as well as las...

Страница 32: ...nations are as shown below 635 mm 675 DF 20 Optional filter added 532 mm 570 DF 20 675 DF 20 Optional filters added 473 mm 530 DF 20 570 DF 20 675 DF 20 Optional filters added See page 99 for the met...

Страница 33: ...Integers only b Dragging the mouse The white area is the scannable area and the red frame area is the scan ning range Drag the red frame area with the mouse to vary the scanning range As the red frame...

Страница 34: ...background components in the slide glass may be reduced in the confocal mode On the contrary unevenness on the slide glass surface may be detected Non Confocal Mode The apparent evenness of images is...

Страница 35: ...and high voltage condition Then click the PreView button 2 2 Preview starts in set conditions In the Preview mode the pixel size is larger and the reading speed is higher than in the final reading 2 3...

Страница 36: ...ng three lasers the FLA 8000 pre scans the sample with these lasers twice each amounting to six times in all 2 4 On completion of PreView the window shown below appears Click the change over button to...

Страница 37: ...Drag the adjuster button below PMT HV The density of the PreView image changes as the adjuster button is dragged Adjust the high volt age to be applied to the PMT with reference to this image density...

Страница 38: ...dition in the pull down menu of the File menu Adialog box opens Input an intended name in the dialog box and save the file Loading the scanning conditions Select Load Condition in the pull down menu o...

Страница 39: ...rt final scanning Scan Mode 1 Slide glass being scanned is marked with Scanned images are displayed in the window in sequence 2 Image window in the Scan mode There are several methods to display the s...

Страница 40: ...2002 Jan Verson 3 0 38 Cascade Displays overlaid images Tile Horizontal Displays images horizontally Scanning Slide Glass Samples Scan Mode...

Страница 41: ...2002 Jan Verson 3 0 39 Tile Vertical Displays images vertically Arrange Icons Windows illustrated as title bars are arranged Scanning Slide Glass Samples Scan Mode...

Страница 42: ...og is selected on the Window menu the minimized catalog window is enlarged and displayed in the forefront 4 Change images scanned by three types of lasers in the slide glass window as shown below Scan...

Страница 43: ...by the blue 473 nm laser Blue red Image scanned by the blue 473 nm laser overlaid on the image scanned by the red 635 nm laser Red green blue Images scanned by the three lasers all overlaid Click on t...

Страница 44: ...displayed image click the button and click the mouse button in a position to be contracted in the image window To move a displayed image without changing the magnification click the button and drag th...

Страница 45: ...n the histogram or by dragging the button below the histogram 6 Click on the Save icon in the Scan mode window or the Save button in the lower left The Scan mode window changes into the Save mode wind...

Страница 46: ...ciple xxx 1_473 Click the button The Preference dialog box shown below is displayed Automatic Suffix Rule No Automatic file naming is not executed The operator should input a file name of each image f...

Страница 47: ...hat character string and the suffix If you input test in the Base Name box for example the file name test1_473 is given to the first one of images scanned by the 473 nm laser Yes repetitive Automatic...

Страница 48: ...IFF file Conversion setting Fixed Images are automatically converted into the TIFF file format using the algorithm in the software based on the maximum value of the images Custom Input a numeric value...

Страница 49: ...his box is saved together with the image data 5 Selecting an image to be saved The file will be saved as long as the check box on the left side of the slide icon is chosen Deselecting the check box by...

Страница 50: ...2002 Jan Verson 3 0 48...

Страница 51: ...2002 Jan Verson 3 0 49 Part 5 Usage of IPs...

Страница 52: ...it into electric signals A radiation image recorded on the IP during exposure is read as digital image information at the maximum resolution of 10 um per pixel 100 pixels mm and recorded in the analyz...

Страница 53: ...vironmental conditions Do not expose IPs to the direct sunlight Do not use them in a place with much ultraviolet rays or natural radiation Do not use them in a hot and humid place Storage Store IPs le...

Страница 54: ...age quality is guaranteed when an IP is scanned by the FLA 8000 immediately after it is exposed Therefore it is important to set the exposure start time and exposure time so that exposure ends just be...

Страница 55: ...d completely In such a case scan it once by way of trial to check if it is erased completely Refer to the manual of the IP eraser The IP eraser may erase an IP in fourteen minutes if it is not exposed...

Страница 56: ...ideoftheIPscanned first i e left side when the IP is set in the FLA 8000 and expose it Partial exposure reduces the scanning time 3 Set the IP on the cassette so that its exposure surface is in contac...

Страница 57: ...eful not to apply an impact to the cassette An impact applied to the cassette may shift the sample and IP Do not expose the IP in a place with much environmental radiation in order to avoid the increa...

Страница 58: ...2002 Jan Verson 3 0 56...

Страница 59: ...2002 Jan Verson 3 0 57 Part 6 Scanning IPs...

Страница 60: ...and Software Setup of Hardware and Software 1 Switch on the FLA 8000 and peripheral devices 2 Switch on the PC 3 Make sure that the FLA 8000 has warmed up and is ready for operation Activate the FLA...

Страница 61: ...e Click on the IP icon or the radio button on the left of the IP icon to select the IP 2 Set the IP on the IP carrier 2 1 Put the IP carrier with the magnet surface upside as shown below Not selected...

Страница 62: ...3 Turn down the room lighting below 20 luxes Keep the lighting turned down until the IP carrier is set and the main cover is closed 2 4 Take the IP out of the IP cassette and set it on the IP carrier...

Страница 63: ...llowing figure shows the relationship between the IP setting position and the image position on the monitor When the IP is viewed from the back as shown below scanning starts at the upper left corner...

Страница 64: ...7 Set the IP carrier Set the IP carrier so that the large hole for removing the IP in the carrier is located on the front side and the poles on the FLA 8000 are engaged with the holes in the carrier S...

Страница 65: ...irmly Close the carrier locking cover so that the poles on both sides of the cover are securely engaged with the pole holds on the FLA 8000 The pole holds are located on the right and left 2 9 Close t...

Страница 66: ...4 The window for setting the scanning conditions appears Setting Mode 1 The following shows the details of the scanning conditions 1 1 Resolution um Resolution Select a pixel scanning size The scanni...

Страница 67: ...nal FUJI B390 filters for scanning IPs in the FLA 8000 before scanning an IP The FLA 8000 Image Reader does not automatically check whether the B390 filters are set If they are not set properly no ima...

Страница 68: ...specified conditions 2 2 Root Folder Option The folder where the scanned data file is stored is displayed in the box below Root Folder Option If you want to change the folder click the button and chan...

Страница 69: ...File menu A dialog box opens Input an intended name in the dialog box and save the file Loading the scanning conditions Select Load Condition in the pull down menu of the File menu A dialog box opens...

Страница 70: ...displayed in the main window and the small window in the display area as soon as it is scanned 2 You may execute the following in the Scan mode 2 1 Skipping or stopping scanning Stop Click this butto...

Страница 71: ...displayed image click the button and click the mouse button in a position to be enlarged in the image window To contract a displayed image click the button and click the mouse button in a position to...

Страница 72: ...you want to change the gradation of displayed images select a scale in the pull down menu and adjust the gradation with the adjuster bar on the histogram or by dragging the button below the histogram...

Страница 73: ...n the Save mode window 1 1 Giving an image file name It is possible to give names to image files automatically in a certain rule Serial numbers are added to the end of image file names Each file name...

Страница 74: ...Automatic file naming is not executed The operator should input a file name of each image file Yes continuative Automatic file naming is executed Suffices are added to file names Select the suffix ty...

Страница 75: ...sts of that character string and the suffix If you input test in the Base Name box for example the file name test1_640 is given to the first one of images scanned by the 640 nm laser Yes repetitive Au...

Страница 76: ...into a TIFF file Conversion setting Fixed Images are automatically converted into the TIFF file format using the algorithm in the software based on the maximum value of the images Custom Input a numer...

Страница 77: ...e Name box The comment input in this box is saved together with the image data 5 Cancelling your saving action If you do not want to save the IP image file read out deselect the check box next to the...

Страница 78: ...2002 Jan Verson 3 0 76...

Страница 79: ...2002 Jan Verson 3 0 77 Part 7 Scanning Gel Samples...

Страница 80: ...1 Switch on the FLA 8000 and peripheral devices 2 Switch on the PC 3 Make sure that the FLA 8000 has warmed up and is ready for operation Activate the FLA 8000 Image Reader from the Startup menu or u...

Страница 81: ...Set Mode 1 Select Gel in Select Sample in the Carrier Set mode Click on the Slide icon or the radio button on the left of the Slide icon to select the gel 2 Set gel on the gel carrier 2 1 Place the ge...

Страница 82: ...The relationship between the gel setting position and the scanned image dis played on the monitor is as shown below Note Be careful not to make the gel buffer solution exceed the gel stopper inside th...

Страница 83: ...2002 Jan Verson 3 0 81 2 3 Open the sample setting door 2 4 Open the carrier locking cover Scanning Gel Samples Carrier Set Mode...

Страница 84: ...cover is located on the left and the poles on the FLA 8000 are engaged with the holes in the carrier 2 6 Close the carrier locking cover firmly Close it so that the poles on both sides of the carrier...

Страница 85: ...door 3 Go back to the PC and click on Setting in the PC window on the right of Carrier Set in the upper part of the window or lower left of the window 4 The window for the setting the scanning condit...

Страница 86: ...to 100 um Note Gel scanning speed The gel scanning speed is fixed to 400 mm second 1 2 Specifying gel to be scanned and laser filter and high voltage conditions Tick the box below an intended gel icon...

Страница 87: ...The filter types available for a laser differ with the laser types The available combi nations are as shown below 635 mm 675 DF 20 Optional filter added 532 mm 570 DF 20 675 DF 20 Optional filters add...

Страница 88: ...You may save and load recall the scanning conditions Saving the scanning conditions Select Save Condition in the pull down menu of the File menu A dialog box opens Input an intended name in the dialog...

Страница 89: ...indow to start final scanning Scan Mode 1 Gel being scanned is marked with Scanned images are displayed in the window in sequence 2 Image window in the Scan mode There are several methods to display t...

Страница 90: ...2002 Jan Verson 3 0 88 Cascade Displays overlaid images Tile Horizontal Displays images horizontally Scanning Gel Samples Scan Mode...

Страница 91: ...2002 Jan Verson 3 0 89 Tile Vertical Displays images vertically Arrange Icons Windows illustrated as title bars are arranged Scanning Gel Samples Scan Mode...

Страница 92: ...2002 Jan Verson 3 0 90 3 When Catalog is selected on the Window menu the minimized catalog window is enlarged and displayed in the forefront Scanning Gel Samples Scan Mode...

Страница 93: ...e Image scanned by the green 532 nm laser overlaid on the image scanned by the blue 473 nm laser Blue red Image scanned by the blue 473 nm laser overlaid on the image scanned by the red 635 nm laser R...

Страница 94: ...a displayed image click the button and click the mouse button in a position to be contracted in the image window To move a displayed image without changing the magnification click the button and drag...

Страница 95: ...d images select a scale in the pull down menu and adjust the gradation with the adjuster bar on the histogram or by dragging the button below the histogram 5 3 Changing the color of displayed images Y...

Страница 96: ...n 3 0 94 6 Click on the Save icon in the Scan mode window or the Save button in the lower left The Scan mode window changes into the Save mode window in which scanned images are saved Scanning Gel Sam...

Страница 97: ...iles automatically in a certain rule Serial numbers are added to the end of image file names Each file name consists of the characters shown in the following example in principle xxx 1_473 Click the b...

Страница 98: ...d im ages Clicking the button resets the suffices added to the previous data and gives the file name xxx 1 to the first one of newly scanned im ages If you input a character string in the Base Name bo...

Страница 99: ...mat Click the button or the File Format tag in the Preference dialog box of the Naming Rule Option described above A dialog box for specifying a file format is displayed You may select one of the foll...

Страница 100: ...tting Fixed Images are automatically converted into the TIFF file format using the algorithm in the software based on the maximum value of the images Custom Input a numeric value which indicates the c...

Страница 101: ...this box is saved together with the image data 5 Selecting an image to be saved The file will be saved as long as the check box on the left side of the gel icon is chosen Deselecting the check box by...

Страница 102: ...2002 Jan Verson 3 0 100...

Страница 103: ...2002 Jan Verson 3 0 101 Setting Optional Filters Part 8 Setting Optional Filters...

Страница 104: ...ndard filters Set the optional filters in the procedures shown below 1 Click on the Option menu to display the pull down menu Select Filter Change 2 Open the filter adding door on the right side of th...

Страница 105: ...tting Optional Filters 4 Remove the filter ring from the filter block using the tool and remove the shield plate 5 Prepare a filter of the following dimensions Diameter 25 mm Thickness 4 mm or more 12...

Страница 106: ...2002 Jan Verson 3 0 104 Setting Optional Filters 7 Set the filter block with the filter on the front side 8 Close the filter adding door...

Страница 107: ...2002 Jan Verson 3 0 105 Part 9 Daily Maintenance...

Страница 108: ...e interval shorter than a month 1 Maintenance of SHG Laser Operation procedures 1 Switch on the FLA 8000 body and analyzer unit 2 Make sure that they have started up Then turn them off CAUTION Activat...

Страница 109: ...2002 Jan Verson 3 0 107 Part 10 Troubleshooting...

Страница 110: ...ective You may not use the specific scanning mode where the defective laser is used Other scanning modes are available 2 Analog circuits are adjusted improperly You may not use the combination of that...

Страница 111: ...ver during the self diagnosis and startup adjustment of the FLA 8000 about fifteen minutes after turning on the power Close the cover CASE 2 Didn t you open the cover during scanning Close the cover i...

Страница 112: ...urs in the FLA 8000 body Beep sound Be beep be beep be beep An error message dialog box is displayed on the Image Reader screen displayed on the analyzer unit Refer to Countermeasures according to war...

Страница 113: ...f diagnosis first when a trouble occurs in order to solve a trouble smoothly Self diagnosis procedures When an error occurs remove the carrier close the cover and execute a self diagnosis without swit...

Страница 114: ...ecuted for about five minutes and a text file having the following file name is created automatically xxxx_DIAG_xxxx 5 The following dialog box is displayed again Click the button 6 Click on Short to...

Страница 115: ...r execute self diagnosis Countermeasures according to warning and error messages 7 When an error code is displayed memorize it and click the OK button A text file having the following file name is cre...

Страница 116: ...ent upper limit A Peltier error bit is detected A thermistor error bit is detected No lasers are installed Close the sample setting door or filter exchanging door If the phenomenon does not change on...

Страница 117: ...interruption is failed while monitoring the inte gral current The integral current could not be adjusted within the target value range A line interruption is failed while monitoring the inte gral volt...

Страница 118: ...out error while moving toward the part with pin holes Pinhole unit error Sensor OFF error after moving toward the part with pinholes Pinhole unit error Time out error while moving toward the part wit...

Страница 119: ...oving to the origin after receiv ing an origin command Sub scanning unit error Time out error while moving out of the origin Sub scanning unit error Sensor ON error after moving out of the origin Sub...

Страница 120: ...rror Sensor ON error after moving a specified filter other than the optional filter Main scanning unit error Time out error while moving after receiving specified pulses or an origin command Sub scann...

Страница 121: ...mand Focusing unit error Exceeding the specified positive position Focusing unit error Exceeding the specified negative position Sub scanning unit error Time out error while moving after receiving spe...

Страница 122: ...e specified number of times Lamp current check error The monitored lamp current is below the standard PMT sensitivity check error The difference between the data of the equipment and the sensitivity f...

Страница 123: ...the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics Moving when fo...

Страница 124: ...user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected...

Страница 125: ...er manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a...

Страница 126: ...the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detec...

Страница 127: ...lem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics...

Страница 128: ...nd run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Ple...

Страница 129: ...ected with an AC outlet properly CASE 2 An abnormal image is displayed A virtually impossible image is displayed An image is displayed in a wrong position Other Troubles 1 Impacts or vibrations are ap...

Страница 130: ...2002 Jan Verson 3 0 128...

Страница 131: ...2002 Jan Verson 3 0 129 Part 11 Specifications...

Страница 132: ...rauschpegelmesser Fuji Kalibrierung Nr 8697 in einer Entfernung von 1 m vom Gerat und einer Hohe von 1 2 m uber dem Boden Major Specifications Image Scanning Specifications Applicable samples Slide gl...

Страница 133: ...100 V Approx 7 A 200 V IP eraser 1 25 A 100 V 0 7 A 200 V Pictorography 12 A 100 V 6 A 200 V 5 Grounding Class D grounding This equipment must be grounded by connecting the power cable plug with a 2P...

Страница 134: ...2002 Jan Verson 3 0 132...

Страница 135: ...2002 Jan Verson 3 0 133 Part 12 Warranty...

Страница 136: ...is installed 4 If the user carries out improper operation or maintenance 5 If a trouble originates from moving or transportation after installation 6 If a trouble is caused by irresistible forces suc...

Страница 137: ...2002 Jan Verson 3 0 135...

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