GELFREE 8100 Fractionation System
(
24 of 30
)
Operating Procedures (cont.)
7.
To ensure proper buffering capacity, Running Buffer must be
replaced in the Anode and Cathode Buffer Reservoirs after
approximately 30 minutes of cumulative run time at 100V or
60 minutes of cumulative run time at 50 V, according to the
following procedure:
a. Remove the cartridge from the instrument and discard
the buffer.
ch Cathode Buffer Reservoir with
8 mL
Running
instrument and resume the
9.
b. Fill each Anode Buffer Reservoir with
8 mL
Running
Buffer.
c. Fill ea
Buffer.
d. Fill the Sample Loading Chambers with
150 µL
Running Buffer.
e. Fill the Sample Collection Chambers with
150 µL
Running Buffer.
f.
Return the cartridge to the
Method.
Repeat the Sample Collection Cha
.
m
8
ber collection, wash and
fill steps for each subsequent fraction.
Once all of the Method’s steps have been completed, the
display screen will indicate that the experiment has ended.
Collect the final fraction and enjoy the benefits of
reproducible, high yield, liquid-phase fractionation in y
our
protein research.
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