MIRI
®
II-12 Multiroom IVF incubators User Manual Rev. 4.0
42
Figure 18.3
4-well dish with 3 buffers and media
For calibration, at least two buffers are needed. We recommended using 3 buffers.
One of the buffers should have a 7 pH. Any pH buffer can be used as the user's buffer
levels can be set in the calibration dialogue window. If only one or two buffers are
available, the system can still be used but with reduced accuracy.
The technique requires the user to be quick, as the pH starts to shift very quickly once the
lid is opened. The optimal time to complete the procedure is tested to be 15 seconds, giving
the same results as the continuous measurement described below.
Press the “Calibrate” key:
Figure 18.4
pH calibration screen view
Set the buffer levels with the (+) and (-) keys to correspond to the buffers used.
Before measuring in the culture media, calibrate the probe in 2 or 3 buffers. It is necessary
to rinse the probe between each insertion.
