Elmi Fugamix3 CM-50MP, Руководство пользователя, Страница: 12 / 24

Miniprep 1
F1 F2 F3
F4 F5 F6
F9 F8 F7
Plasmid DNA
11
Operation
Miniprep plasmid DNA extraction mode mapped on a standard
protocol and a standard kit.
Operating fugamix in “Miniprep 1” mode
Mixer
12
Timer
20
sec
F2
Timer
RPM
00000
off
Mixer
00
Timer off
F1
Timer
RPM
0 1
13500
min
Load test tubes containing bacteria in to the rotor. Press Start/Stop
and wait 1 min until the cells will be separated. Remove supernatant.
Step 1
Add 250 μ l of resuspension buffer. Press Start/Stop.
Within 20 sec all the precipitate will resuspend.
Step 2
Mixer
0 1
Timer
40
sec
F4
Timer
RPM
13500
Mixer
0 1
Timer
F3
Timer
RPM
10
0 1000
sec
Add 250 μ l of lysys buffer.Press Start/Stop. Two solutions will
now mix homogeneously while cell lyses.
Step 3
Add 350 μ l of neutralization buffer. Press Start/Stop.
Two solutions will mix homogeneously and neutralize. Followed by
5 min spin down. Proteins and DNA is now separated.
Step 4
Mixer
00
Timer off
F5
Timer
RPM
20
13500
sec
Transfer the supernatant to a spin column and press Start/Stop.
20 sec spindown will gather plasmid DNA on the column.
Step 5
20
sec
0 5
min