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UNPACKING THE WIRE MYOGRAPH SYSTEM

UNPACKING THE WIRE MYOGRAPH SYSTEM

Please take a few minutes to carefully inspect your new Wire Myograph System for damage which may have occurred during
handling and shipping. If you suspect any kind of damage, please contact DMT immediately and the matter will be pursued as
quickly as possible. If the packing material appears damaged, please retain it until a possible claim has been settled.

We recommend that you store the packing material for any possible future transport of the Wire Myograph System. Please
contact DMT Sales Department for packing instructions if the original packing material is unavailable.

After unpacking your new Wire Myograph System, please use the following list to check that the system is complete:

Wire Interface

• Power supply (the shape of the AC plug varies by country; be sure that the plug has the right shape for your location)

Confocal Wire Myograph - 360CW or Wire Myograph - 320A

• Myograph connection cable with a temperature probe

• Plastic mounting jaws (360CW) or stainless steel mounting jaws with supports (320A)

• Chamber cover

Accessories:

• 1 x calibration kit (including bridge, T-balance and 2 gram weight)

• 1 x funnel

• 1 x stainless steel wire 40 µm

• 2 x Allen keys (360CW) or 3 x Allen keys (320A)

• 1 x high vacuum grease

• 1 x grease for protection of linear slides

• 4 x spare screws (360CW) for mounting of jaws or 5 x spare screws (320A)

• 1 x screwdriver

• 10 x spare cover glass od 12,0 x 0,17 mm

Optional Accessories:

• PowerLab

• LabChart

• DMT Device Enabler

• Stimulator

• pH meter

• Vacuum Pump

• Electronic vacuum valve

• Waste bottle

• Gas supply manifold

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Содержание 320A

Страница 1: ...USER MANUAL VOL 2 0 WIRE MYOGRAPH SYSTEM 360CW 320A ...

Страница 2: ...User Manual Version 1 0 WIRE MYOGRAPH SYSTEMS 360CW AND 320A USER MANUAL ...

Страница 3: ...es the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the Wire Myograph system No part of this document may be reproduced by any means without the prior written permission of D...

Страница 4: ...orce calibration 10 Chapter 3 The wire Interface Menus 11 3 1 General description on how to navigate the touch screen 11 3 2 Power up screen 11 3 3 Main Menu 12 3 4 Zero Menu 12 3 5 Heat Menu 13 3 6 Timer and Buzzer Menu 13 3 7 Settings Menu 14 3 7 1 Force Calibration Menu 14 3 7 2 pH Calibration Procedure 18 3 7 3 Select Analog Output optional 19 3 7 4 Interface Settings 20 3 7 4 1 Temperature Di...

Страница 5: ...adapter Protect the power adapter and cord from being walked on or pinched Particularly at power plugs and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has spilled onto or objects have fallen into the apparatus the apparatu...

Страница 6: ... please use the following list to check that the system is complete Wire Interface Power supply the shape of the AC plug varies by country be sure that the plug has the right shape for your location Confocal Wire Myograph 360CW or Wire Myograph 320A Myograph connection cable with a temperature probe Plastic mounting jaws 360CW or stainless steel mounting jaws with supports 320A Chamber cover Acces...

Страница 7: ...ce rear ON OFF switch 24V DC port for power Connection port for Wire Myograph Analogue recorder output connection to external data acquisition software optional USB connection port for computer Connection port for pH meter optional Grounding connect to Data Acquisition Figure 1 1 Wire Interface front Power indicator Touch display ...

Страница 8: ...chamber 25 pin serial cable for communication with Wire Interface Allen screws for fine alignment of the wire jaws Wire jaw connected to force transducer Force transducer pin Vertical micrometer screw Wire jaw connected to horizontal micrometer Horizontal micrometer 1 2 Wire Myographs 1 2 1 Confocal Wire Myograph 360CW ...

Страница 9: ...cted to micrometer Micrometer Suction pipe for connection to vacuum pump Pipe for gas supply Pipe for filling the chamber using the 40 mm funnel Window at the bottom of the chamber for imaging under the wire jaws Force transducer pin Figure 1 1 Single Wire Myograph with close up of chamber Transducer house Allen screws for fine alignment of the wire jaws 2 on each side ...

Страница 10: ...erface to computer connection Data acquisition is possible either by connecting the Wire Interface directly to a computer or through a Data Acquisition System able to collect voltage output 0 2 5volts such as a PowerLab Data Acquisition System or similar system Direct computer USB connection for Labchart AD Instruments users latest version of Labchart Connect the Wire Interface to the computer wit...

Страница 11: ...power to the Wire Interface at the power switch and then turn on the computer Start the data acquisition pro gram on the computer and the Wire Myograph System is now ready for use 4 Gas supply Connect the pipes for gas supply on the Confocal Wire Myograph 360CW chamber cover see figure 2 3 to an adjustable gas supply using thin silicone tubing For the Wire Myograph 320A connect the pipe for gas su...

Страница 12: ...arameter can be done by pressing UP or DOWN arrows Once the desired setting has been chosen pressing ENTER will lock the selection and be stored in memory Pressing the white X in the red box will exit the menu and take you automati cally to the Main Menu 3 2 Power up screen After turning on the Wire Interface an introduction screen appears It dis plays the product system model no software version ...

Страница 13: ... the Heat is OFF If the icon is green the Heat is ON active Four sub menus are accessible from the Main Menu screen Zero Menu Heat Menu Timer Alarm Menu Setting Menu Icon for Heater ON OFF Timer Icon for Buzzer ON OFF If ON the icon is green Zero Menu Heat Menu Timer Alarm Menu Settings Menu OFF ON 00 00 00 OFF ON 3 3 Main Menu The Main Menu gives a good overall status on how the Wire Interface is...

Страница 14: ...ess ON and it will turn green The system will heat to the desired temperature setpoint In the Main Menu the heat icon turns green when the heat is on 3 6 Timer and Buzzer Menu The timer and buzzer are controlled from this menu Use SELECT to program the timer The timer can be programmed in the interval from 0 to 23 59 59 Hours minutes seconds When Set Timer is selected use the UP and DOWN arrows to...

Страница 15: ...the Settings Menu The calibration procedure is listed in 6 indi vidual steps and needs to be performed to calibrate the system Before starting the Force calibration the following is performed Place the calibration bridge and T balance on the Wire Myograph see figure 3 1 for 360CW or 3 2 for 320A allowing it to be warmed up together with the Wire Myograph Turn on the heating in the Heat sub menu to...

Страница 16: ...owing placement of the calibration bridge and T balance for Wire Myograph 320A Figure 3 1 Force calibration set up showing placement of the calibration bridge and T balance with weight in place for Wire Myograph 360CW The 2 g weight is placed on the pan ...

Страница 17: ... Step 3 Initiates the heating process for the chambers if not already done In order for the calibration to be accurate the transducers must be heated to the experimental temperature to be used This accommodates heat in duced expansion of the electronic parts in the transducer Otherwise inac curate readings and transducer drift may occur producing errors into the experiment Covering the chamber wit...

Страница 18: ...ACED IN ADDITION IF THE FORCE READING S APPEAR YEL LOW CANNOT BE RESET TO ZERO AND THE TRANSDUCER CANNOT BE RECALIBRATED THE FORCE TRANSDUCER IS BROKEN AND NEEDS TO BE REPLACED Step 5 At this step place the 2 gram weight at the pan on the calibration T balance closest to the transducer over the transducer see figure 3 1 or 3 2 to simulate the stretch created by the contraction of a mounted ring pr...

Страница 19: ...mode the temperature of the calibration buffers is measured with a thermometer and entered manually in the Manual temperature line The calibration procedure is listed in 4 individual steps and needs to be performed one by one to calibrate the pH Step 1 Involves cleaning the pH electrode and the temperature probe with double distilled water When ready press NEXT STEP Step 2 Place the pH electrode a...

Страница 20: ... of 2 5V to 2 5V Use SELECT to select the out channel number to be changed Press ENTER to go to the output set up Use SELECT to select the line to be changed Use the UP and DOWN arrow or DEFAULT to change select a new set up The following parameters in the Wire Interface can be selected as output to the analog channels Force Near Force Far Temperature Probe Chamber Temperature pH1 Optional Digital...

Страница 21: ... set up menu the two pH values used for the 2 point pH calibration should be selected The default in the pH Calibration setup is the pH values 4 and 7 If using pH buffer with different pH values than 4 and 7 for the pH calibration enter the appropri ate pH The temperature is an important parameter in the calibration formula and is obtained automatically if the temperature compensation it is set to...

Страница 22: ...ependent of superfusion Temp range Ambient temp 50 C Temp resolution 0 1 C Temp probe External Output reading Force mN Analogue output Up to four outputs 1 0V full scale for all acquired signals user defined Serial output Serial interface RS232 RS485 Voltage 100 to 240 VAC auto 50 60Hz Ambient temp 15 to 30 C Technical specifications Single Wire Myograph 320A Vessel size 60 μm Chamber Single bath ...

Страница 23: ...E MYOGRAPH SYSTEM 360CW 320A USER MANUAL Optional accessories Enable pH meter on the Wire Interface range pH 0 14 temp correction 0 50 C Electronic valve 100 to 240 VAC auto 50 60 Hz via external power supply ...

Страница 24: ...24 NOTES NOTES ...

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