Troubleshooting Guide
Problem
Things to try:
Low DNA recovery
Run some of the untreated and RapidTip
purified PCR on a gel to check your
amplicon band intensity.
Note: Purify at least 20 uL of your PCR reaction
to optimize DNA recovery.
Slow or difficult
aspiration
Check that pipettor volume is 50 uL
Check that tips are firmly attached to the
pipettor
Note: Diffinity RapidTip is incompatible
with detergents, mineral oil, and ready load
PCR mixes that contain density increasing
compounds.
Fluid remains in the
RapidTip
It is normal for a small amount of liquid
(~ 5 uL) to remain inside the Diffinity
RapidTip.
Note: Overdispense or blowout all fluid on the
last dispense cycle.
Failure to remove
impurities
Verify that particles are at the bottom of
the tip prior to sample treatment; the
pipettor is operating at the proper (slow)
speed settings; and that particles are
effectively mixing with the sample during
pipetting.
Note: Remove trapped air bubbles by tapping
the tip or mix for additional aspirate/dispense
cycles.
Diffinity RapidTip
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