8
Programs C, D & E
–
Procedure for Testing Low Levels of Water in Oil Using a 5 mL
Sample Size
(Reagent System: HS-LRP)
1.
PREPARATION:
Remove a sampling syringe and a white-capped reaction tube from the reagent box. Check
the contents to ensure that all items are present and intact. Remove the white cap from the reaction tube and
discard. Carefully remove a green water ampule from the cardboard carton and insert it fully into the top of the
protective ampule sleeve containing the grayish white ampule.
Do not replace the protective ampule sleeve
into the tube at this time.
2.
SAMPLING:
Using a clean polyethylene sampling pipette, pipette 5 mL of the oil to be tested into the reaction
tube, e.g., up to 5 mL line marked on side of the reaction tube
(NOTE: The protective ampule sleeve is not to
be in the tube at this time.)
. Insert the protective ampule sleeve into the reaction tube with the grayish white
ampule on the bottom, then insert the black septum (hole on top) into the reaction tube by gripping the tube
firmly and pressing down on the septum with your thumb until flush with top of the tube.
TIGHTLY
cap the
tube with the holed, white cap.
NOTE:
A homogeneous, representative sample of the oil being tested must
be used to obtain accurate results.
DO NOT SQUEEZE THE SIDES OF THE TUBE WHILE
INSERTING THE BLACK SEPTUM. DOING THIS WILL RESULT IN LOW READINGS.
3.
REACTION:
Break the bottom (grayish white) ampule in the reaction tube by compressing the sides of the
tube. Mix thoroughly by shaking the reaction tube vigorously for 30 seconds. Allow the reaction to proceed for
a total of 4 minutes. The mixture will bubble and perhaps foam during this time.
DO NOT BREAK THE
GREEN AMPULE.
(NOTE: If the test sample is very thick and/or viscous, shake the reaction tube again for
30 seconds and let stand for an additional 4 minutes.)
4.
ANALYSIS:
Press the <ON> key once to activate the meter. Ensure the meter is set for the correct program C,
D or E for the sample matrix being tested (See HydroSCOUT Manual on “Selecting a Program” for instructions
on how to change programs.). Once the meter displays
“
tUbE
”, insert the reaction tube into the opening at the
bottom of the meter. The hole in the white cap must be lined up with the metal pin extending from the inside of
the hole of the meter. Push the reaction tube all the way into the meter. Press the <READ> key on the face of
the meter. The display will flash the word “
CALC
,” and the results will be displayed
micrograms of water
per milliliter of sample.
Record the results.
KEEP THE METER AND REACTION TUBE UPRIGHT AT
ALL TIMES.
5.
DISPOSAL:
Carefully remove the reaction tube from the meter. Vent the reaction tube by inserting it
completely into the specially designed venting cap unit and remove.
REACTION TUBE AND CAP MUST BE
UPRIGHT AND POINTED AWAY FROM USER AND BYSTANDERS.
Tap the reaction tube on a hard
surface to shake any loose glass to the bottom of the tube, then break the top (green) ampule in the reaction tube
by compressing the sides of the reaction tube. Shake the tube for 15 seconds. After 5 minutes, vent the tube
again. Discard the reaction tube as laboratory waste.
DO NOT REMOVE WHITE CAP.
6.
CALCULATION:
The meter is programmed to display the result in
micrograms per milliliter
(µg/mL)
. If a
result is to be reported as a weight/weight value, divide the HydroSCOUT result by the density of the oil.
Water in Oil (µg/g) = R/d
where:
R = Meter reading in µg/mL
d = Density of oil in g/mL
