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TE62 Standard Transfer Tank

Operating Instructions

Original instructions

Содержание TE62

Страница 1: ...TE62 Standard Transfer Tank Operating Instructions Original instructions cytiva com ...

Страница 2: ...ansfer 26 6 Maintenance 29 7 Troubleshooting 31 8 Referenceinformation 35 8 1 Specifications 36 8 2 Recyclinginformation 37 8 3 Regulatoryinformation 38 8 3 1 Contactinformation 39 8 3 2 EuropeanUnionandEuropeanEconomic Area 40 8 3 3 EurasianEconomicUnion Евразийскийэкономическийсоюз 41 8 3 4 DeclarationofHazardousSubstances DoHS 43 8 4 Orderinginformation 45 8 5 HealthandSafetyDeclarationForm 46 ...

Страница 3: ...nsimportantuserinformation descriptionsofsafetynotices and intendeduseoftheTransphorinstrument Inthischapter Section Seepage 1 1 Aboutthismanual 4 1 2 Importantuserinformation 5 1 Introduction TE62StandardTransferTankOperatingInstructions29281927AB 3 ...

Страница 4: ...ating InstructionscovertheTransphorinstrument Theillustrationbelow showstheTransphorinstrument Illustrations Theimagesandannotationsinthisdocumentareforillustrativepurposesonly The configurationofindividualproductsmayvary andthereforeillustrationsmaynot reflecttheactualsystemdelivered 1 Introduction 1 1 Aboutthismanual 4 TE62StandardTransferTankOperatingInstructions29281927AB ...

Страница 5: ...erbuffertemperaturecanbecontrolledbycirculatingcooledliquidthrough theheatexchangerinthebase Thebufferisseparatedfromthecoolantbyaheatcon ductingaluminaplate Transphormustnotbeusedinanyclinicalprocedures orfordiagnosticpurposes Prerequisites InordertooperateTransphorinthewayitisintended Theusermusthaveageneralunderstandingofelectrophoresisandblottingtechni ques TheusermustreadandunderstandtheSafet...

Страница 6: ...ncyandrecoveryprocedures Important WARNING Beforeinstalling operatingormaintainingtheproduct all usersmustreadandunderstandtheentirecontentsofthis chaptertobecomeawareofthehazardsinvolved Inthischapter Section Seepage 2 1 Safetyprecautions 7 2 2 Labels 13 2 3 Emergencyprocedures 14 2 Safetyinstructions 6 TE62StandardTransferTankOperatingInstructions29281927AB ...

Страница 7: ...waysfollowtheinstructionsbelowtoavoidinjurywhenusingtheTransphor Definitions Thisuserdocumentationcontainssafetynotices WARNING CAUTION andNOTICE concerningthesafeuseoftheproduct Seedefinitionsbelow WARNING WARNINGindicatesahazardoussituationwhich ifnotavoided couldresultindeathorseriousinjury Itisimportantnottoproceed untilallstatedconditionsaremetandclearlyunderstood CAUTION CAUTIONindicatesahaz...

Страница 8: ...G Donotdamagethepowersupplycordbybending twisting heating orallowingittobecomepinnedundertheequipment Using damagedpowercordscouldresultinfireorelectricshock Ifthepowersupplycordsaredamaged contactyourlocalCytiva representativeforreplacements WARNING Thesafetylidmustbeinplacebeforeconnectingthepowerleads toapowersupply WARNING Anyliquidontheequipmentmustbedriedoffbeforeconnecting thepowersupply CA...

Страница 9: ...oduct WARNING Spreadofbiologicalagents Theoperatormusttakeallneces saryactionstoavoidspreadinghazardousbiologicalagents The facilitymustcomplywiththenationalcodeofpracticefor biosafety CAUTION Handletheglasscomponentswithcare Wearappropriate personalprotectiveequipment PPE Usingflammableliquids WARNING Afumehoodorsimilarventilationsystemshallbeinstalledwhen flammableornoxioussubstancesareused Inst...

Страница 10: ...vedbyCytiva WARNING Makesurethatthereisaccesstotheinstrumentpowersupplycord atalltimes Operation WARNING Thehighvoltagepowersupplymustalwaysbedisconnectedwhen thesafetylidoftheelectrophoresisunitistakenoff Thehigh voltagepowersupplymustneverbeswitchedonunlessthesafety lidisontheelectrophoresisunit WARNING Neverexceedtheoperatinglimitsstatedinthisdocumentandon thesystemlabel Operationoftheproductou...

Страница 11: ...rtaporanycoolantsource wherethewaterpressureisunregulated CAUTION Circulateonlywateror50 50water ethyleneglycolthroughthe heatexchanger Neveruseanti freezeoranyorganicsolventinthe heatexchanger CAUTION Neverintroduceanti freezeoranyorganicsolventintoanypartof theinstrument Organicsolventswillcauseirreparabledamageto theinstrument CAUTION Bufferabovethemaximumbufferlevellinemaycausecorrosionof thee...

Страница 12: ...dparts Onlysparepartsandaccessoriesthat areapprovedorsuppliedbyCytivamaybeusedformaintainingor servicingtheproduct WARNING Disconnectpower Alwaysdisconnectpowerfromtheinstru mentbeforeperforminganymaintenancetask WARNING Decommissioning Decontaminatetheequipmentbefore decommissioningtoensurethathazardousresiduesareremoved 2 Safetyinstructions 2 1 Safetyprecautions 12 TE62StandardTransferTankOperat...

Страница 13: ...lystated intheuserdocumentation Serialno Serialnumberoftheproduct Manufac tured Year YYYY andmonth MM ofmanufacture Safetylabels Thetablebelowdescribesthevarioussymbolsthatmaybefoundontheproduct Symbol text Description Warning Readtheuserdocumentationbeforeusing thesystem Donotopenanycoversorreplaceparts unlessspecificallystatedintheuserdocumentation Warning HighVoltage Alwaysmakesurethatthe syste...

Страница 14: ...encyshutdown Inanemergencysituation shutdownthepowersupplyinaccordancewithitsemer gencyprocedure Powerfailure Incaseofpowerfailuretotheproduct therunisinterruptedimmediately Restartafteremergencyshutdownor powerfailure Torestarttherunafteranemergencyshutdownorpowerfailure followthesesteps Step Action 1 Makesureallconnectionsareinplace 2 Startthepowersupplyasdescribedinthepowersupply sUserManual 2 ...

Страница 15: ...chaptergivesanoverviewoftheTransphor andabriefdescriptionofitsfunction Illustrationoftheinstrument Theillustrationbelowshowsthemainpartsoftheinstrument 1 2 3 4 5 6 7 3 Systemdescription TE62StandardTransferTankOperatingInstructions29281927AB 15 ...

Страница 16: ... 5 Tankfilllevels 6 Coolantports 2 7 Coolantsafetyvalve Illustrationofthetransferstack assembly Theillustrationbelowshowsthemainpartsoftheinstrument 6 1 2 3 4 5 Part Function 1 Sponge 3mm 2 Blottingpaper 3 Membrane 4 Gel 3 Systemdescription 16 TE62StandardTransferTankOperatingInstructions29281927AB ...

Страница 17: ...Part Function 5 Blottingpaper 6 Sponge 3mm forgels 1 5mm Sponge 6mm forgels 1 5mm 3 Systemdescription TE62StandardTransferTankOperatingInstructions29281927AB 17 ...

Страница 18: ...thatthesystemisplacedonastable levelbenchwith adequatespaceforventilation Unpackingprocedure Unwrapallpackagescarefully Inspectallvisiblepartsfordamageormissingpieces Ifanydamageisobserved record thisonthereceivingdocumentsandinformyourCytivarepresentative Makesureto keepallpackingmaterialfordamageclaimsortouseshoulditbecomenecessaryto returntheunit 4 Installation 18 TE62StandardTransferTankOperat...

Страница 19: ...umentandon thesystemlabel Operationoftheproductoutsidetheselimitscan damageequipmentandcausepersonalinjuryordeath WARNING Turnallpowersupplycontrolsoffanddisconnectthepowerleads beforeremovingthesafetylid WARNING Rinseonlytheelectrodes notthebananaplugs withdistilled waterbeforeuse CAUTION Circulatecoolantthroughtheheatexchangertominimizeheating Overheatingwillcauseirreparabledamagetotheunit Donot...

Страница 20: ...inthe heatexchanger CAUTION Neverintroduceanti freezeoranyorganicsolventintoanypartof theinstrument Organicsolventswillcauseirreparabledamageto theinstrument Inthischapter Section Seepage 5 1 Preparations 21 5 2 Assembly 23 5 3 Electrotransfer 26 5 Operation 20 TE62StandardTransferTankOperatingInstructions29281927AB ...

Страница 21: ...th a stirrer to avoid buffer depletion at the electrodes Note Connect the heat exchanger to a circulator bath Note The circulator pump must not generate a pressure greater than 0 7 bar g 10 psi above atmospheric pressure Followtheinstructionsbelowtopreparethecoolingoftheunit Step Action 1 Attachatubingtotheredpressurereliefvalve seeIllustration of the instru ment on page 15 2 Insertthefreeendofthe...

Страница 22: ...tteswithdistilledwater 2 Place donotdrop amagneticstirringbarinthebuffertank Dropping objectsontothealuminaplateintheTransphortankmaycausetheplateto crack 3 Placetheinstrumentonamagneticstirrer 4 FilltransferbuffertotheSTARTFILLLEVELline Thisrequiresapproxi mately3 8L 5 Setthestirrertolow medium Thiscreatesbuffercirculationwithoutforcing bufferthroughtheassembledcassettes 5 Operation 5 1 Preparati...

Страница 23: ... 3 Soakallmembranetypesintransferbufferfor2to5minutes 4 Openthecassettebyreleasingbothlatchtabsalongtheedgeoppositethe hinges 5 Placetheopenedcassetteintoatrayfilledwithatleast3cmoftransfer buffer Assembletransfercassette Toaidtheassembly seeIllustration of the transfer stack assembly on page 16 NOTICE Whenassemblingthetransfercassette useonlytherequired amountofgelsupportmaterials spongesandblott...

Страница 24: ...o place the gel correctly the first time because proteins may begin to transfer immediately once transfer has begun moving the gel will distort results or cause shadow bands on the blot 5 Gentlyrollaglasspipetoratesttubeoverthegeltoexpeltrappedair betweenthemembraneandgel 6 Coverthegelwithasheetofblottingpaper 7 Gentlyrollaglasspipetoratesttubeoverthestacktoexpeltrappedair betweentheblottingpapera...

Страница 25: ...ette s nearthetank 2 Liftoutonecassetteatatimeandslideitintoasetofverticalslots Donot discardthebuffer 3 Onceinplace tapthecassettelightlyuntilmostairbubblesaredislodged A fewsmallbubblesinthespongesareunlikelytointerferewiththetransfer 4 Inspectthebufferlevel Makesurethatthebufferlevelfallsbetweenthe minimumandmaximumbufferlevellines SeeIllustration of the instrument on page 15 andAssemble transf...

Страница 26: ...ntofthepresenceofSDS andundermostconditionsnucleicacids arenegativelychargedandalsomigratetowardtheanode Note The cassettes are color coded to match the leads in the lid Coolingisstronglyrecommended Anysettingthatresultsinhigherthan5Wofpower willgenerateenoughheattorequireactiveheatcontrol Arefrigeratedcirculatorbath shouldbesettocooltoabout10 C ifusing50 50ethyleneglycol water thetempera turecanb...

Страница 27: ...culesorthickergelsmayrequirelongertransfer times Theoptimumtransfertimeforeachsystemmustbedetermined empirically Transferslefttorunovernightshouldbesettoaconstant currentsettingnohigherthan0 1A Afterthetransferiscomplete Thissectiondescribeshowtoremovethegelandthemembranefromthetransfer stack Step Action 1 Turnthevoltageandcurrentsettingstozeroandturnoffthepowersupply 2 Disconnecttheleadsfromthepo...

Страница 28: ...ges 8 Rinsetheunitimmediatelyafteruse SeeGeneral cleaning procedure on page 29 Note Do not store used buffer in the transfer tank Chill buffer to 10 C before reuse 5 Operation 5 3 Electrotransfer 28 TE62StandardTransferTankOperatingInstructions29281927AB ...

Страница 29: ...Keeptheexterioroftheinstrumentdryandclean Wipe regularlywithasoftdamptissueand ifnecessary amildcleaning agent Lettheinstrumentdrycompletelybeforeuse Generalcleaningprocedure WhencleaningtheTransphormakesureto notautoclaveorheatanypartabove45 C notexposeittoorganicsolvents abrasives strongcleaningsolutions orstrong acidsorbasestocleanthechambers neveruseabrasivedetergents decontaminatetheunitwitha...

Страница 30: ...thepanelout 5 Carefullyplacethenewpanelinitsplace Takecaretonotbreakthe retaininglip Cleaningbeforeplanned maintenance service Toensuretheprotectionandsafetyofservicepersonnel allequipmentandworkareas mustbecleanandfreeofanyhazardouscontaminantsbeforeaServiceEngineer startsmaintenancework PleasecompletethechecklistintheOn Site Service Health and Safety Declaration FormortheHealth and Safety Declar...

Страница 31: ...dintransfer buffer gentlypressoneachspongeasit isaddedtothestack androllaglass pipetteortesttubeoverthemembrane andgeltoeliminateallairbubbles Reducethestirringspeedtoprevent turbulence Processonlyonestripormembranein eachtrayorcassettetopreventover lapping Usebufferwithalowerionicstrength Checkelectrodecontinuity Duringthe transfer acontinuousstreamofgasis releasedalongtheentirelengthofthe electr...

Страница 32: ...transfer Useathinnergel Reducethegelacrylamideconcentra tion CheckthatthebufferpHisclosetothe intendedpH Mostbuffersshouldnotbe titrated makefreshbuffer Use3 5mMSDS 0 1 inthetransfer buffer Avoidincludingmethanolinthe transferbufferorreducetheamountto theabsoluteminimum Usereagent gradechemicals IncreasethelengthoftimeSouthern blotsaredepurinated Increasethenetchargeontheprotein bychangingtoatrans...

Страница 33: ...k slightly large molecules may migrate more slowly Takecarethatthegelisheldfirmly againstthemembraneandthatitdoes notshiftoncecontactismade Ifexcessheatingoccursduringthe transfer lowerthetemperatureofthe coolingfluidintheheatexchanger Checkthatthepreferredbinding surfaceofthemembrane ifany contactsthegel Problemswithinefficientbindingto membrane Errordescription Correctiveaction Chemicalparameter...

Страница 34: ...okeepthe membranesactivated Useamembranewithasmallerpore size 0 10to0 20μm ifproteinspass throughthemembrane orusea differentmembranetype Placeamembranebothoverandunder thegelifyoususpectoneproteinis movingintheoppositedirectionfrom themajorityoftheproteins Checkboth membranesforprotein s Checkiftoomuchsampleisavailable forthebindingsurfaceareabyapplying twomembranesinsteadofone If blow through oc...

Страница 35: ...Thechapteralsoincludesorderinginformation andHealth andSafetyDeclarationformsforservice Inthischapter Section Seepage 8 1 Specifications 36 8 2 Recyclinginformation 37 8 3 Regulatoryinformation 38 8 4 Orderinginformation 45 8 5 HealthandSafetyDeclarationForm 46 8 Referenceinformation TE62StandardTransferTankOperatingInstructions29281927AB 35 ...

Страница 36: ...e 15 21cm Numberofcassettes 4 Buffervolume 4to5L dependingonthenumberof cassettesinplace Installationcategory II Environmentalrequirements Parameter Requirement Allowedlocation Indooruseonly Ambienttemperature operation 4 Cto40 C Max relativehumidity operation Upto80 RH Altitude operation Upto2000m Pollutiondegree 2 8 Referenceinformation 8 1 Specifications 36 TE62StandardTransferTankOperatingInst...

Страница 37: ...uipment Disposaloftheproduct Whentakingtheproductoutofservice thedifferentmaterialsmustbeseparatedand recycledaccordingtonationalandlocalenvironmentalregulations Disposalofelectricalcomponents Wasteelectricalandelectronicequipmentmustnotbedisposedofasunsortedmunic ipalwasteandmustbecollectedseparately Pleasecontactanauthorizedrepresenta tiveofthemanufacturerforinformationconcerningthedecommissioni...

Страница 38: ...section Section Seepage 8 3 1 Contactinformation 39 8 3 2 EuropeanUnionandEuropeanEconomicArea 40 8 3 3 EurasianEconomicUnion Евразийскийэкономическийсоюз 41 8 3 4 DeclarationofHazardousSubstances DoHS 43 8 Referenceinformation 8 3 Regulatoryinformation 38 TE62StandardTransferTankOperatingInstructions29281927AB ...

Страница 39: ...facturinginformation Thetablebelowsummarizestherequiredmanufacturinginformation Requirement Information Nameandaddressofmanufacturer CytivaSwedenAB Björkgatan30 SE75184Uppsala Sweden Telephonenumberofmanufacturer 46771400600 8 Referenceinformation 8 3 Regulatoryinformation 8 3 1 Contactinformation TE62StandardTransferTankOperatingInstructions29281927AB 39 ...

Страница 40: ...ncludedwiththeproduct acopyoftheEUDeclarationofConformityisavailable onrequest CEmarking TheCEmarkingandthecorrespondingEUDeclarationofConformityisvalidforthe instrumentwhenitis usedaccordingtotheOperating Instructionsorusermanuals and usedinthesamestateasitwasdelivered exceptforalterationsdescribedinthe Operating Instructionsorusermanuals 8 Referenceinformation 8 3 Regulatoryinformation 8 3 2 Eur...

Страница 41: ...facturerand importer inaccordancewiththerequirementsoftheTechnicalRegulationsofthe CustomsUnionand or theEurasianEconomicUnion Requirement Information Name addressandtelephone numberofmanufacturer SeeManufacturing information Importerand orcompanyfor obtaininginformationabout importer CytivaRUSLLC 109004 Moscow internalcityareaTaganskymunicipal district Stanislavskystr 21 building3 premisesI offic...

Страница 42: ...s cytiva com Descriptionofsymbolonthesystem label Описаниеобозначениянаэтикетке системы ThisEurasiancompliancemarkindicatesthattheproductis approvedforuseonthemarketsoftheMemberStatesofthe CustomsUnionoftheEurasianEconomicUnion ДанныйзнакоЕвразийскомсоответствииуказывает что изделиеодобренодляиспользованиянарынках государств членовТаможенногосоюзаЕвразийского экономическогосоюза 8 Referenceinforma...

Страница 43: ...roducts 电子信息产品污染控制标志说明 ExplanationofPollutionControl Label 该标志表明本产品不含有超过中国标准GB T26572 电子信息产品中有毒有害物质的限量要求中限量的有毒有害物质报废后可以进行回收处理不能随意丢弃 Thissymbolindicatesthatthiselectricalandelectronicproductdoesnotcontainanyhazardous substancesabovethemaximumconcentrationvalueestablishedbytheChinesestandardGB T 26572 andcanberecycledafterbeingdiscarded andshouldnotbecasuallydiscarded 8 Referenceinformation 8 3 ...

Страница 44: ...要求此表所列数据为发布时所能获得的最佳信息 0 Indicatesthatthishazardoussubstancecontainedinallofthehomogeneous materialsforthispartisbelowthelimitrequirementinGB T26572 X Indicatesthatthishazardoussubstancecontainedinatleastoneofthe homogeneousmaterialsusedforthispartisabovethelimitrequirementin GB T26572 Datalistedinthetablerepresentsbestinformationavailableatthetimeof publication 8 Referenceinformation 8 3 Regulato...

Страница 45: ...8 4 Ordering information Forproductcodesandinformationabouthowtoorder pleaseseecytiva com 8 Referenceinformation 8 4 Orderinginformation TE62StandardTransferTankOperatingInstructions29281927AB 45 ...

Страница 46: ... cases this may require customer to move equipment from normal operating location prior to Cytiva arrival Consumables such as columns or gels have been removed or isolated from the instrument and from any area that may impede access to the instrument All buffer waste vessels are labeled Excess containers have been removed from the area to provide access Provide explanation for any No answers here ...

Страница 47: ...t be decontaminatedprior to service return Provide a telephone number where Cytiva can contact you for additional information concerning the system equipment Telephone No Liquid and or gas in equipment is Water Ethanol None empty Argon Helium Nitrogen Liquid Nitrogen Other specify Equipment type Product No Serial No I hereby confirm that the equipment specified above has been cleaned to remove any...

Страница 48: ...n 45 P Powerfailure 14 Prepare 21 buffer 21 Transphor 21 Prerequisites 5 Productreturnorservicing 47 Purposeofthismanual 4 R Recyclinginformation 37 decontamination 37 disposalofelectricalcompo nents 37 Regulatoryinformation 38 S Safetylabel 13 Description 13 Safetynotices 7 Safetyprecautions 7 9 Flammableliquids 9 introduction 7 System 36 dimensions 36 specifications 36 weight 36 Systemlabel 13 D...

Страница 49: ... instrumentmainparts 15 Troubleshooting 31 33 diffusebandpatterns 33 incompletetransfer 31 inefficientbinding 33 U Unpacking 18 Unpackingprocedure 18 Index TE62StandardTransferTankOperatingInstructions29281927AB 49 ...

Страница 50: ...pectiveowners 2020 2021Cytiva AllgoodsandservicesaresoldsubjecttothetermsandconditionsofsaleofthesupplyingcompanyoperatingwithintheCytiva business Acopyofthosetermsandconditionsisavailableonrequest ContactyourlocalCytivarepresentativeforthemostcurrent information Forlocalofficecontactinformation visitcytiva com contact 29281927 AB V 5 04 2021 ...

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