Cole Parmer 78904-00, Инструкция по установке и эксплуатации

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3. Adjusting the diopter
•
Set diopter adjustment ring to zero
•
Close the left eye and focus the right tube by adjusting the coarse- and fine adjustment knobs
•
Close the right eye and focus the left tube with the diopter adjustment ring
This procedure should be followed by each individual user. When more users are working on the same microscope it is
recommended to remember your own diopter setting for a quick set-up during new microscopy sessions
Abbe condenser
Beneath the object stage an Abbe condenser N.A. 1.25 is mounted. The condenser can be adjusted in height by
moving the lever beneath the mechanical stage. By adjusting the condenser you can focus the light on the specimen
for an optimized contrast. The condenser is factory pre-centered. If needed, the following procedure can be followed
to center the condenser
1.
Move the condenser to its highest position
2.
Select the 10x objective and place it into the light path and focus on the specimen
3.
Rotate the field diaphragm adjustment ring to put the field diaphragm to the smallest position
4.
Adjust the condenser to get the sharpest image
5.
Adjust the center adjustment screw and center the image in the field of view
6.
Open the field diaphragm gradually
7.
The condenser is centered correctly if the image remains in the center when you open the field diaphragm
The field (Köhler) diaphragm
By limiting the diameter of the beam entering the condenser, the field diaphragm can prevent stray light and increase
the image contrast. When the image is right on the edge of the field of view, the objective shows the best performance
and obtains the clearest image. The diaphragm is factory pre-centered
Adjusting the aperture diaphragm
1.
The aperture diaphragm is used to select the
numerical aperture of the illumination. When the
N.A. of the illumination matches with the N.A.
of the objective, you get the highest possible
resolution, depth of field and contrast
2.
When contrast is low, rotate the diaphragm
adjustment ring to 70%-80% of the N.A. of
objective. This will improve the contrast of the
image. The diaphragm is factory pre-centered
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