How to Perform Total Cell Count and
Viability of Heavily Aggregated Cells
Select the protocol from the menu
‘Viability and Cell Count -
A100
and
B
Assay’.
Optional:
Enter Sample ID and Operator Name.
Retain an aliquot of at least 200 µl of the original cell suspension for analysis in step 5.
Add
equal volumes of
Reagent
A100
and your cell sample to a microcentrifuge tube and mix.
For example, to 100 µl of cell suspension add 100 μl of
Reagent
A100
.
Afterwards add one
volume of
Reagent
B
to the mixture of cell suspension and
Reagent
A100
. To 200 µl of the
mixture of cell suspension and
Reagent
A100
add 100 μl of
Reagent
B
and mix by pipetting
i.e. Cell Sample:
Reagent
A100
:
B
, should be mixed in a ratio of 1:1:1.
Immediately load your sample into a Via1-Cassette™ and press ‘RUN’.
After analysis, follow the on-screen instructions and load a sample of your cells NOT treated
with
Reagent
A100
and
B
into a new Via1-Cassette™ and press ‘RUN’.
Compensation for the sample dilution with
Reagent
A100
and
B
is automatically performed by the
software. However, if the proportions are different from those above, please add the correct volumes
in the input fields under the image window prior to running your assay. The entered volumes are
used for calculating the cell concentration in the original cell sample.
