Carolina 3M Petrifilm ColiformCount Plates Скачать руководство пользователя страница 3

3

Storage

Refrigerate unopened packages at 

8˚C (

46˚F). Use before expiration date

on package.

To seal an opened package, fold the end over and tape it shut (fig. 1).

Figure 1

Keep resealed packages at room temperature and less than 50% relative
humidity. Do not refrigerate opened packages. Use plates from the opened
package within one month after opening.

Directions for Use

Use sterile technique when handling Petrifilm plates. Disinfect the work area
by wiping thoroughly with alcohol or other disinfectant before and after use.
Used Petrifilm plates may contain viable organisms. Do not open the plates
unnecessarily. The procedures in this instruction booklet should be used for
educational purposes only.

Method A. Inoculation with liquid sample

Inoculate and spread each Petrifilm plate before going on to the next plate.

1. If a Petrifilm plate pack has been stored in the refrigerator, let the

package come to room temperature before opening it. This step
prevents condensation from forming inside the package.

Содержание 3M Petrifilm ColiformCount Plates

Страница 1: ...82 4030 3M Petrifilm Coliform Count Plates Instruction Manual CAROLINA Teamed with Teachers...

Страница 2: ...for counting the viable organisms present See Method A Petrifilm plates can be hydrated first with water or buffer then inoculated by swabbing streaking or touching to surfaces See Method B Antibioti...

Страница 3: ...g Petrifilm plates Disinfect the work area by wiping thoroughly with alcohol or other disinfectant before and after use Used Petrifilm plates may contain viable organisms Do not open the plates unnece...

Страница 4: ...cular to the Petrifilm plate place 1 mL of sample onto the center of the bottom film fig 3 If necessary samples can be diluted with distilled water liquid culture medium or buffers with pH between 6 6...

Страница 5: ...gure 4 5 With the flat side down not the side with the circular ridge place the spreader on the top film over the inoculum fig 5 Figure 5 6 Gently apply pressure on the spreader to distribute the inoc...

Страница 6: ...tion time and temperature will vary according to the application and equipment available 9 Colonies on Petrifilm plates can be counted on a standard colony counter or other light source fig 8 Bacteria...

Страница 7: ...diluents that contain citrate or sodium thiosulfate because they can inhibit growth on the Petrifilm plates These substances are not found in common microbiological media such as Luria broth or nutri...

Страница 8: ...media such as Luria broth or nutrient broth Interpretation The U S Food and Drug Administration Bacteriological Analytical Manual defines coliforms as gram negative rods that produce acid and gas from...

Страница 9: ...9 Sample Plate Figure 12 Figure 11 Gas bubbles produced by colonies Gas bubble not associated with a colony...

Страница 10: ...BiologicalSupplyCompany 2700 York Road Burlington North Carolina 27215 CB162760002 To order call 1 800 334 5551 US and Canada 336 584 0381 International For technical help call 1 800 227 1150 www caro...

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