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Single Slide Automated Gram Stainer User Manual

15

Art specifications

:

IFU/Manual - 8"Wx10"H

Do not alter art or size.

All art prints PMS 185 Red and 

100% Black.

AMS1800062
Rev. 0  2018-06

9.2.12 Press [ 2 ]

 on the key pad to select 

Gram Stain (LONG)

9.2.13

 Place a clean, blank slide in the cuvette when the screen prompt reads 

Load Slide

9.2.14 Press any key

 on the key pad, except 

[ * ]

, to commence the Bleach Cycle

9.2.15

 When the cycle is complete, Press 

[ * ]

 on the key pad to return to the Main Menu

9.2.16 Press [ 1 ]

 on the key pad to select 

Run

 

9.2.17 Press [ 4 ]

 on the key pad to select 

Purge

9.2.18 Press [ * ] 

on the key pad to return to the Main Menu

9.2.19 Empty the 10% bleach solution

 from the DI Water container, and 

refill the water

 container with fresh,  

supplemented deionized water (refer to section 9.5). 

9.2.20

 Wipe off and place 

all of the cannulas

 into the water container now full of fresh deionized water.

9.2.21 Press [ 1 ]

 on the key pad to select 

Run

 

 

9.2.22 Press [ 3 ]

 on the key pad to select 

Prime

9.2.23 Press [ * ]

 on the key pad to return to the Main Menu

9.2.24 Press [1]

 on the key pad to select 

Run

9.2.25 Press [1]

 on the key pad to select 

GRAM STAIN

9.2.26 Press [ 2 ]

 on the key pad to select 

Gram Stain (LONG)

9.2.27

 Place a new blank slide in the cuvette when the screen prompt reads 

Load Slide

9.2.28 Press any key

 on the key pad, except 

[ * ]

, to commence the Rinse Cycle

9.2.29 Press [ * ]

 on the key pad to return to the Main Menu

9.2.30 Press [ 1 ]

 on the key pad to select 

Run

 

9.2.31

 Lift all of the cannulas out of the water container and place them into the waste container before the next step.

9.2.32 Press [ 4 ]

 on the key pad to select 

Purge

9.2.33

 Wipe off and 

replace all of the cannulas

 back into their respective reagent and/or water containers.

9.3 Scrub Cycle

The Scrub feature for the Single Slide Automated Gram Stainer is required every 15 days or 600 cycles, whichever occurs first.  
The unit will automatically prompt the operator when scrub cycle needs to be run. The total time to run a Scrub Cycle is five minutes. 

9.3.1

 From the main menu, 

Press [ 1 ] 

on the key pad to select 

Run

.

CHBAGS_GramStainer_IFU.indd   15

7/3/18   1:31 PM

Содержание CHB-AGS

Страница 1: ...Wx10 H Do not alter art or size All art prints PMS 185 Red and 100 Black AMS1800062 Rev 0 2018 06 CHB AGS Cardinal Health Single Slide Automated Gram Stainer Instructions for Use Manual CHBAGS_GramSt...

Страница 2: ...Reagent Kit Installation 7 6 0 OPERATIONS INSTRUCTIONS 8 6 1 Instrument Anatomy 8 6 2 Specimen Slide Preparation 8 6 3 Instrument Preparation 9 6 4 Stain Operation 10 7 0 QUALITY CONTROL 12 7 1 Qualit...

Страница 3: ...nce Summary 18 10 0 IDLE DELAYS 18 10 1 Setting the Sleep Delay 19 11 0 UNIT SHUT DOWN 19 11 1 Unit Shut Down Instructions 19 12 0 SERVICES AND SUPPLIES 20 13 0 SAFETY DATA SHEETS 20 14 0 REFERENCES 2...

Страница 4: ...th its quality management system which complies with the U S Food and Drug Administration s FDA s Quality Systems Regulation QSR and current Good Manufacturing Practices cGMP contained in Title 21 Par...

Страница 5: ...7 63Hz Temperature Ambient Operation 15 30C Humidity 0 to 95 without condensation Operator Adjustments Individual adjustable stain times on both short and long cycles Adjustable sleep mode Adjustable...

Страница 6: ...in of the Gram s method consists of staining with crystal violet which is then fixed with iodine The microorganisms that retain the crystal violet iodine complex appear purple under microscopic examin...

Страница 7: ...afety protection features to function as designed The power supply is equipped with one attached power cord and one removable power cord The attached power cord is equipped with a connector that plugs...

Страница 8: ...also not recommended Methanol fixed slides have been shown to retain two to ten times as many cells than with heat fixation 1 In addition Gram positive bacteria are much less likely to become over de...

Страница 9: ...tions vary according to jurisdiction Please dispose of waste according to your facility and local regulations 6 3 4 Confirm that deionized water is present in the supply container and make sure that t...

Страница 10: ...4 5 Select a Stain Delay Option Select one of the two stain delays 1 Gram Stain SHORT or 2 Gram Stain LONG by pressing the 1 or 2 key on the key pad to choose the desired stain delay times for the run...

Страница 11: ...the Run Menu 7 0 QUALITY CONTROL 7 1 Quality Control Checks The quality and validity of the Gram stain process of the Single Slide Automated Gram Stainer may be periodically verified by staining a sl...

Страница 12: ...iration Date into the appropriate fields as it reads on the new reagent kit label 8 1 12 Press on the key pad to select Accept and save the kit registration information 8 1 13 Press on the key pad onc...

Страница 13: ...New Pump Replacement Tube Kit 8 3 1 From the Main Menu Press 2 on the key pad to select SET UP 8 3 2 Press 2 on the key pad to select Consumables 8 3 3 Press 2 on the key pad to select Review Tubing 8...

Страница 14: ...ry month or 600 cycles whichever occurs first The unit runs a 10 bleach solution through the reagent and water lines to clean the internal surface of the tubing and to prevent debris build up in the w...

Страница 15: ...the key pad to select Prime 9 2 23 Press on the key pad to return to the Main Menu 9 2 24 Press 1 on the key pad to select Run 9 2 25 Press 1 on the key pad to select GRAM STAIN 9 2 26 Press 2 on the...

Страница 16: ...f the unit 9 3 6 Press 1 on the key pad to select Scrub The unit will automatically prime the system cleaner into the reagent lines and then begin the scrub sequence 9 3 7 During the scrub sequence us...

Страница 17: ...the water cannula from the water container and place it onto a fresh paper towel or alcohol swab 9 6 2 Fill the water container with fresh deionized water and mix in the drops of Gram s Iodine 9 6 3...

Страница 18: ...y a complete prime of the reagent lines This prevents reagent evaporation in the supply lines and maintains the unit in a ready state without wasting reagents as each of the reagents is expelled back...

Страница 19: ...press any key to continue NOTE For the Scrub procedure it is recommended that the System Cleaner for Single Slide Automated Gram Stainer Cat No CHB AGS SC is used Alternatively decolorizer can be used...

Страница 20: ...x 16 fl oz CHB GS QC QC Slides 50 pack CHB AGS SC System Cleaner 2 x 500ml CHB AGS RT Replacement Tubing Kit 8 tubes kit CHB GS CS Cuvette Cleaning Swabs 100swabs kit CHB GS DI Gram s Iodine for DI Wa...

Страница 21: ...by Cardinal Health viii Use of microscope slides other than the chemically treated Cardinal Health Microscope Slide Charged Adhesion Frosted 25mm x 75mm 1mm Cat No M6143 or Hardy Diagnostic PROBond C...

Страница 22: ...every 20 days 20 slides or every 8 days whichever comes first Every 15 days or every 600 slides whichever occurs first 6 Months or every 1 100 cycles whichever occurs first DeionizedWaterand IodineDi...

Страница 23: ...tem trained Trainee s Initials _____ Specimen Slide Preparation Section 6 2 _____ Loading Slides Section 6 4 6 _____ Initiating a Gram Stain Section 6 4 _____ Register Reagent Kit Section 8 1 _____ Ge...

Страница 24: ...2018 06 CHB AGS Distributed by Cardinal Health Waukegan IL 60085 USA 2018 06 cardinalhealth com 2018 Cardinal Health All Rights Reserved CARDINAL HEALTH and the Cardinal Health LOGO are trademarks of...

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