Q u i c k S t a r t
MULTI
CAM
VERSION:
AUG 2017 (SN 7072 - PRESENT)
The MultiCam is a powerful and stable device for pixel aligning images captured using different wavelengths, polarization
states, fields of view or other imaging modalities onto up to four camera sensors. It is designed to meet the most demanding
requirements for the sub-pixel mapping of Super Resolution imaging and is compatible with most scientific C mount and F
mount cameras.
Please contact us if you require any assistance during setup or use ([email protected]).
For the purpose of this Quick Start Guide, please refer to the MultiCam diagram on Page 3. All words in
bold blue
text are
labelled on this diagram.
1.
Connect the MultiCam to your existing 1x camera c-mount.
2.
If using an inverted microscope, three support jacks are normally supplied to support the weight of the unit. Position
the support jacks in the
jacking point
on each output. The height can be adjusted and locked in position with the blue
locking collar.
3.
Remove the
C-mount camera tube
and protective
dust cap
. The
clamping screw
may need to be loosened (using the
2.5mm allen key provided)
4.
Screw each camera onto the
C-mount camera tube
and replace on each arm of the MultiCam with the
calibration ring
still in place.
A) Mounting to your microscope
email: [email protected] [email protected]
+44(0)1795 590140 www.cairn-research.co.uk
B) Mounting and positioning four cameras
1.
Identify the
transmitted port (1)
on the MultiCam diagram
2.
View a live image on the corresponding camera (ideally of a graticule using transmitted light) on the transmitted port.
For initial setup, it is essential to be able to view all four edges of the rectangular aperture, therefore it is not advisable
to use a fluorescent sample at this stage.
3.
Close the
rectangular aperture
until in view on the image and rotate the camera until square.
4.
Lock off the
clamping screw
5.
Insert the calibration cubes (each containing a 50% mirror) into the three
combiner cubes
by removing the magnetic
door covers
. Magnets will ensure the cube locates correctly.
6.
Repeat steps 1 to 4 on the remaining camera ports.
7.
If the sample focus on any of the other cameras is not precisely matching the focus of the transmitted camera, an
adjustment is provided (to allow for any slight differences between positioning of the camera sensor). Remove the
calibration ring
and move the camera in Z until focused. Lock off the
clamping screw
once again.
C) Centre the transmitted image
1.
With the
rectangular aperture
still in view, centre the
transmitted port (1)
(using the 1.5mm allen key provided) by
adjusting the
transmitted H&V
controls. It’s useful to use a centred square or central cross-hair as a reference point
in your imaging software (if available).
