Gemini Series Electroporator User’s Manual
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There are many kinds of buffers and medium used for
electroporating cells. Typically, we recommend using
medias without serum or antibiotics.
The following is a list of the most commonly used buffers/
medium:
BTXpress
– is a single buffer solution, developed to quickly and
efficiently deliver genes into mammalian cells that were previously
considered “hard to transfect” by chemical and other non-viral
methods. This solution, in combination with BTX electroporators,
provides researchers with the versatility needed for success
across a broad range of cell types while maintaining critical cell
viability. Transfection using this high performance electroporation
solution is equally effective in delivering DNA as well as siRNA into
mammalian cells.
PBS
– is a buffer solution commonly used in biological research. It
is a water-based salt solution containing sodium chloride, sodium
phosphate, and, in some formulations, potassium chloride and
potassium phosphate. The buffer’s phosphate groups help to
maintain a constant pH. The osmolarity and ion concentrations of
the solution usually match those of the human body (isotonic).
HEPES
– is widely used in cell culture, largely because it is better
at maintaining physiological pH despite changes in carbon dioxide
concentration (produced by cellular respiration) when compared to
bicarbonate buffers, which are also commonly used in cell culture.
RPMI
– is a form of medium used in cell culture and tissue culture.
It has traditionally been used for growth of Human lymphoid cells.
This medium contains a great deal of phosphate and is formulated
for use in a 5% carbon dioxide atmosphere.
Opti-MEM
– is an improved Minimal Essential Medium (MEM)
that allows for a reduction of Fetal Bovine Serum supplementation
by at least 50% with no change to growth rate or morphology.
Opti-MEMt can be used with a variety of suspension and adherent
mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and
primary fibroblasts.
MEM Eagle
– is suitable for a diverse spectrum of mammalian cell
types. Various formulations available with either Hank’s or Earle’s
salts.
DMEM
– MEM is used in a wide range of mammalian cell culture
applications. The high glucose version is well suited to high density
suspension culture. The low glucose formula is used for adherent
dependent cells.
CytoMix
– is a composition of cytokines for the highly efficient
and reproducible expansion of human multipotent mesenchymal
stromal cells (MSCs).
Water & 10% glycerol
– Typically used for bacteria
Cytoporation Media T
– is a buffer designed for larger volume
cell electroporation as it incorporates a low conductivity of 0.08 S/
cm to reduce heating of solution during electroporation.
Cytoporation Media T4
– is a buffer designed for larger volume
cell electroporation as it incorporates a low conductivity of 3.45
mS/cm to reduce heating of solution during electroporation.
Electroporation Buffers
